Investigation of enhancement effects of nicotine on cholinergic neurotransmission in isolated rabbit gastric fundus: role of antioxidants

Nicotine, which is tobacco alkaloid, still induces interests for researchers because of smokers addiction to nicotine. Nicotine having influence on the neuronal acetylcholine receptors (nAChRs) increases release of most certain neurotransmitters from the nerve endings. Also, nicotine, affecting the...

Full description

Saved in:

Bibliographic Details
Published in:	Autonomic & autacoid pharmacology Vol. 30; no. 3; p. 179
Main Authors:	Anuvarbekova, A, Fincan, G S O, Vural, I M, Ozger, S I, Ercan, Z S, Utkan, T, Sarioglu, Y
Format:	Journal Article
Language:	English
Published:	England 01-07-2010
Subjects:	Acetylcholine - metabolism Acetylcholine - secretion Allopurinol - pharmacology Animals Antioxidants - metabolism Atropine - pharmacology Cholinergic Fibers - drug effects Deferoxamine - pharmacology Electric Stimulation Gastric Fundus - drug effects In Vitro Techniques Mannitol - pharmacology Muscle Contraction - drug effects Muscle, Smooth - drug effects Neostigmine - pharmacology Nicotine - pharmacology Rabbits Reactive Oxygen Species - metabolism Synaptic Transmission - drug effects Tetrodotoxin - pharmacology
Online Access:	Get more information
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	Nicotine, which is tobacco alkaloid, still induces interests for researchers because of smokers addiction to nicotine. Nicotine having influence on the neuronal acetylcholine receptors (nAChRs) increases release of most certain neurotransmitters from the nerve endings. Also, nicotine, affecting the mitochondrial respiratory chains, contributes to the formation of reactive oxygen species. In the present study, we investigated the effects of nicotine on smooth muscles of gastric fundus on the electrical field stimulation (EFS) that induces transition contraction via stimulation nAChRs. In addition, we aimed to investigate the interaction between release of acetylcholine, induced by nicotine, and the effects of reactive oxygen species. Therefore, the effects of allopurinol (10(-6)-10(-5) M), deferoxamine (10(-4) M) and mannitol (10(-4)-5 x 10(-3) M) were tested on the transient contraction induced by nicotine. In conclusion, mannitol (5 x 10(-3) M) significantly reduced contractile response to nicotine on EFS only in high concentration. Whereas in small concentrations mannitol (10(-4) M) statistically did not cause any results. Deferoxamine and allopurinol also did not have any significant response.
ISSN:	1474-8673
DOI:	10.1111/j.1474-8673.2009.00448.x