Search Results - "Ozaki, Shogo"
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Highly organized DnaA-oriC complexes recruit the single-stranded DNA for replication initiation
Published in Nucleic acids research (01-02-2012)“…In Escherichia coli, the replication origin oriC consists of two functional regions: the duplex unwinding element (DUE) and its flanking DnaA-assembly region…”
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DnaB helicase is recruited to the replication initiation complex via binding of DnaA domain I to the lateral surface of the DnaB N-terminal domain
Published in The Journal of biological chemistry (07-08-2020)“…The DNA replication protein DnaA in Escherichia coli constructs higher-order complexes on the origin, oriC, to unwind this region. DnaB helicase is loaded onto…”
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Regulation of replication initiation: lessons from Caulobacter crescentus
Published in Genes & Genetic Systems (01-10-2019)“…Chromosome replication is a fundamental process in all domains of life. To accurately transmit genetic material to offspring, the initiation of chromosome…”
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A novel mode of DnaA–DnaA interaction promotes ADP dissociation for reactivation of replication initiation activity
Published in Nucleic acids research (02-12-2019)“…Abstract ATP-DnaA is temporally increased to initiate replication during the cell cycle. Two chromosomal loci, DARS (DnaA-reactivating sequences) 1 and 2,…”
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IHF and Fis as Escherichia coli Cell Cycle Regulators: Activation of the Replication Origin oriC and the Regulatory Cycle of the DnaA Initiator
Published in International journal of molecular sciences (18-07-2023)“…This review summarizes current knowledge about the mechanisms of timely binding and dissociation of two nucleoid proteins, IHF and Fis, which play fundamental…”
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Novel Divisome-Associated Protein Spatially Coupling the Z-Ring with the Chromosomal Replication Terminus in Caulobacter crescentus
Published in mBio (28-04-2020)“…Cell division requires proper spatial coordination with the chromosome, which undergoes dynamic changes during chromosome replication and segregation. FtsZ is…”
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Differentiation of the DnaA-oriC Subcomplex for DNA Unwinding in a Replication Initiation Complex
Published in The Journal of biological chemistry (26-10-2012)“…In Escherichia coli, ATP-DnaA multimers formed on the replication origin oriC promote duplex unwinding, which leads to helicase loading. Based on a detailed…”
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Escherichia coli CrfC Protein, a Nucleoid Partition Factor, Localizes to Nucleoid Poles via the Activities of Specific Nucleoid-Associated Proteins
Published in Frontiers in microbiology (07-02-2019)“…The CrfC protein is an important regulator of nucleoid positioning and equipartition. Previously we revealed that CrfC homo-oligomers bind the clamp, a…”
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Expression and Genetic Activation of Cyclic Di-GMP-Specific Phosphodiesterases in Escherichia coli
Published in Journal of bacteriology (01-02-2016)“…Intracellular levels of the bacterial second messenger cyclic di-GMP (c-di-GMP) are controlled by antagonistic activities of diguanylate cyclases and…”
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Activation and polar sequestration of PopA, a c‐di‐GMP effector protein involved in Caulobacter crescentus cell cycle control
Published in Molecular microbiology (01-11-2014)“…Summary When Caulobacter crescentus enters S‐phase the replication initiation inhibitor CtrA dynamically positions to the old cell pole to be degraded by the…”
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Z-Ring-Associated Proteins Regulate Clustering of the Replication Terminus-Binding Protein ZapT in Caulobacter crescentus
Published in mBio (26-01-2021)“…Regulated organization of the chromosome is essential for faithful propagation of genetic information. In the model bacterium , the replication terminus of the…”
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Read-through transcription of tRNA underlies the cell cycle-dependent dissociation of IHF from the DnaA-inactivating sequence datA
Published in Frontiers in microbiology (28-02-2024)“…Timely initiation of chromosomal DNA replication in is achieved by cell cycle-coordinated regulation of the replication origin, , and the replication…”
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A Common Mechanism for the ATP-DnaA-dependent Formation of Open Complexes at the Replication Origin
Published in The Journal of biological chemistry (28-03-2008)“…Initiation of chromosomal replication and its cell cycle-coordinated regulation bear crucial and fundamental mechanisms in most cellular organisms. Escherichia…”
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DnaA structure, function, and dynamics in the initiation at the chromosomal origin
Published in Plasmid (01-09-2009)“…Escherichia coli DnaA is the initiator of chromosomal replication. Multiple ATP-DnaA molecules assemble at the oriC replication origin in a highly regulated…”
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The DnaA AAA+ Domain His136 Residue Directs DnaB Replicative Helicase to the Unwound Region of the Replication Origin, oriC
Published in Frontiers in microbiology (31-08-2018)“…Chromosomal replication initiation requires dynamic mechanisms in higher-order nucleoprotein complexes that are constructed at the origin of replication. In ,…”
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Cyclic di-GMP mediates a histidine kinase/phosphatase switch by noncovalent domain cross-linking
Published in Science advances (01-09-2016)“…Histidine kinases are key components of regulatory networks in bacteria. Although many of these enzymes are bifunctional, mediating both phosphorylation and…”
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The interaction of DiaA and DnaA regulates the replication cycle in E. coli by directly promoting ATP DnaA-specific initiation complexes
Published in Genes & development (15-08-2007)“…Escherichia coli DiaA is a DnaA-binding protein that is required for the timely initiation of chromosomal replication during the cell cycle. In this study, we…”
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A Replicase Clamp-Binding Dynamin-like Protein Promotes Colocalization of Nascent DNA Strands and Equipartitioning of Chromosomes in E. coli
Published in Cell reports (Cambridge) (01-09-2013)“…In Escherichia coli, bidirectional chromosomal replication is accompanied by the colocalization of sister replication forks. However, the biological…”
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W13-01 Architecture and mechanism of the initiation complex of the E.coli chromosomal replication
Published in Genes & Genetic Systems (2011)“…The E.coli chromosomal origin oriC binds 10-20 molecules of ATP-DnaA, forming a highly organized nucleoprotein complex, an initiation complex. This complex…”
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The exceptionally tight affinity of DnaA for ATP/ADP requires a unique aspartic acid residue in the AAA+ sensor 1 motif
Published in Molecular microbiology (01-12-2006)“…Summary Escherichia coli DnaA, an AAA+ superfamily protein, initiates chromosomal replication in an ATP‐binding‐dependent manner. Although DnaA has conserved…”
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