Presence of EBV antigens detected by a sensitive method in pediatric and adult Diffuse Large B‐cell lymphomas

In 2017, the World Health Organization (WHO) confirmed a new entity, Epstein Barr virus (EBV) + Diffuse large B cell lymphoma (DLBCL), not otherwise specified (NOS). Traces of EBV transcripts were described in lymphomas, including DLBCL, that were diagnosed as EBV negative by conventional methods. T...

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Published in:	International journal of cancer Vol. 153; no. 5; pp. 1043 - 1050
Main Authors:	Mangiaterra, Tamara S., De Dios Soler, Marcela, Oviedo, Noelia, Colli, Sandra, Preciado, Maria V., Soria, Marcela, Galluzo, Laura, De Matteo, Elena, Chabay, Paola
Format:	Journal Article
Language:	English
Published:	Hoboken, USA John Wiley & Sons, Inc 01-09-2023 Wiley Subscription Services, Inc
Subjects:	Adult Antigens Argentina B-cell lymphoma Cancer Child DLBCL EBV Epstein-Barr virus Epstein-Barr Virus Infections Epstein-Barr Virus Nuclear Antigens - genetics Genomes Herpesvirus 4, Human - genetics Humans LMP1 LMP1 protein Lymphoma Lymphoma, Large B-Cell, Diffuse - pathology Medical research Pediatrics traces Tumor cells Tumors Viral Matrix Proteins - genetics Argentina EBV traces LMP1 DLBCL
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Summary:	In 2017, the World Health Organization (WHO) confirmed a new entity, Epstein Barr virus (EBV) + Diffuse large B cell lymphoma (DLBCL), not otherwise specified (NOS). Traces of EBV transcripts were described in lymphomas, including DLBCL, that were diagnosed as EBV negative by conventional methods. The aim of this study was to detect viral genome by qPCR, as well as LMP1 and EBNA2 transcripts, with a more sensitive method in DLBCL cases from Argentina. Fourteen cases originally considered as EBV negative expressed LMP1 and/or EBNA2 transcripts. In addition, LMP1 and/or EBNA2 transcripts were also observed in bystander cells. However, EBERs+ cells cases by conventional ISH showed higher numbers of cells with LMP1 transcripts and LMP1 protein. In the cases that were EBERS− in tumor cells but with expression of LMP1 and/or EBNA2 transcripts, the viral load was below the limit of detection. This study provides further evidence that EBV could be detected in tumor cells by more sensitive methods. However, higher expression of the most important oncogenic protein, LMP1, as well as increased viral load, are only observed in cases with EBERs+ cells by conventional ISH, suggesting that traces of EBV might not display a key role in DLBCL pathogenesis. What's new? Epstein‐Barr virus (EBV) may be involved in lymphoma pathogenesis more widely than recognized so far. This study provides further evidence that EBV may be detected in tumor cells from lymphomas diagnosed as EBV negative by conventional methods through more sensitive techniques, in a cohort from a population with a high incidence of EBV infection in children. However, since higher expression of the most important oncogenic protein, LMP1, and increased viral load were only observed in cases with EBERs+ cells by conventional in situ hybridization, EBV traces might not play a key role in Diffuse large B‐cell lymphoma pathogenesis.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0020-7136 1097-0215
DOI:	10.1002/ijc.34623