Search Results - "Oveimar Barbosa"

Strategies for the one-step immobilization–purification of enzymes as industrial biocatalysts by Barbosa, Oveimar, Ortiz, Claudia, Berenguer-Murcia, Ángel, Torres, Rodrigo, Rodrigues, Rafael C., Fernandez-Lafuente, Roberto

“…In this review, we detail the efforts performed to couple the purification and the immobilization of industrial enzymes in a single step. The use of…”
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Immobilization of lipases on hydrophobic supports: immobilization mechanism, advantages, problems, and solutions by Rodrigues, Rafael C., Virgen-Ortíz, Jose J., dos Santos, José C.S., Berenguer-Murcia, Ángel, Alcantara, Andres R., Barbosa, Oveimar, Ortiz, Claudia, Fernandez-Lafuente, Roberto

“…Lipases are the most widely used enzymes in biocatalysis, and the most utilized method for enzyme immobilization is using hydrophobic supports at low ionic…”
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Microbial Lipases and Their Potential in the Production of Pharmaceutical Building Blocks by Godoy, César A, Pardo-Tamayo, Juan S, Barbosa, Oveimar

“…Processes involving lipases in obtaining active pharmaceutical ingredients (APIs) are crucial to increase the sustainability of the industry. Despite their…”
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Immobilization/Stabilization of Ficin Extract on Glutaraldehyde-Activated Agarose Beads. Variables That Control the Final Stability and Activity in Protein Hydrolyses by El-Hocine Siar, Sara Arana-Peña, Oveimar Barbosa, Mohammed Nasreddine Zidoune, Roberto Fernandez-Lafuente

“…Ficin extract has been immobilized on different 4% aminated-agarose beads. Using just ion exchange, immobilization yield was poor and expressed activity did…”
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The slow-down of the CALB immobilization rate permits to control the inter and intra molecular modification produced by glutaraldehyde by Barbosa, Oveimar, Torres, Rodrigo, Ortiz, Claudia, Fernandez-Lafuente, Roberto

“…► CALB has been reversibly immobilized on octyl Sepharose at different rates. ► Different percentages of the amino groups in the enzyme have been modified with…”
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Synthesis and Characterization of Cross-Linked Aggregates of Peroxidase from Megathyrsus maximus (Guinea Grass) and Their Application for Indigo Carmine Decolorization by Perez, Angie V, Gaitan-Oyola, Jorge A, Vargas-Delgadillo, Diana P, Castillo, John J, Barbosa, Oveimar, Fernandez-Lafuente, Roberto

“…We present the synthesis of a cross-linking enzyme aggregate (CLEAS) of a peroxidase from (Guinea Grass) (GGP). The biocatalyst was produced using 50% /…”
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Reversible Immobilization of Lipases on Heterofunctional Octyl-Amino Agarose Beads Prevents Enzyme Desorption by Rueda, Nazzoly, Albuquerque, Tiago L, Bartolome-Cabrero, Rocio, Fernandez-Lopez, Laura, Torres, Rodrigo, Ortiz, Claudia, Dos Santos, Jose C S, Barbosa, Oveimar, Fernandez-Lafuente, Roberto

“…Two different heterofunctional octyl-amino supports have been prepared using ethylenediamine and hexylendiamine (OCEDA and OCHDA) and utilized to immobilize…”
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Evaluation of styrene-divinylbenzene beads as a support to immobilize lipases by Garcia-Galan, Cristina, Barbosa, Oveimar, Hernandez, Karel, dos Santos, Jose C S, Rodrigues, Rafael C, Fernandez-Lafuente, Roberto

“…A commercial and very hydrophobic styrene-divinylbenzene matrix, MCI GEL® CHP20P, has been compared to octyl-Sepharose® beads as support to immobilize three…”
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Further Stabilization of Alcalase Immobilized on Glyoxyl Supports: Amination Plus Modification with Glutaraldehyde by Hussain, Fouzia, Arana-Peña, Sara, Morellon-Sterling, Roberto, Barbosa, Oveimar, Braham, Sabrina Ait, Kamal, Shagufta, Fernandez-Lafuente, Roberto

“…Alcalase was immobilized on glyoxyl 4% CL agarose beads. This permitted to have Alcalase preparations with 50% activity retention versus Boc-l-alanine…”
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Immobilization of CALB on activated chitosan: Application to enzymatic synthesis in supercritical and near-critical carbon dioxide by dos Santos, José C.S., Bonazza, Horacio L., de Matos, Leonardo J.B.L., Carneiro, Elizabete A., Barbosa, Oveimar, Fernandez-Lafuente, Roberto, Gonçalves, Luciana R.B., de Sant’ Ana, Hosiberto B., Santiago-Aguiar, Rílvia S.

“…[Display omitted] •Lipase B from Candida antarctica immobilized on activated chitosan.•The loading capacity of the new biocatalyst is around 20mg/g of…”
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Combined effects of ultrasound and immobilization protocol on butyl acetate synthesis catalyzed by CALB by Alves, Joana S, Garcia-Galan, Cristina, Schein, Mirela F, Silva, Alexandre M, Barbosa, Oveimar, Ayub, Marco A Z, Fernandez-Lafuente, Roberto, Rodrigues, Rafael C

“…It is well established that the performance of lipase B from Candida antarctica (CALB) as catalyst for esterification reactions may be improved by the use of…”
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Morphological, Structural, and Functional Evaluation of Rice Starch Acylated in a System Catalyzed by the B‐Lipase of Candida antarctica by Montoya, Diego, Barbosa, Luis Oveimar, Méndez, Jonh, Murillo, Walter

“…Starch modification by acylation induces significant changes in the mechanical and thermal properties of this polymer, widening the scope of its applicability…”
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Elicitor Activity of Curdlan and Its Potential Application in Protection of Hass Avocado Plants against Phytophthora cinnamomi Rands by Guarnizo, Nathalie, Álvarez, Andree, Oliveros, Diego, Barbosa, Oveimar, Joli, Jordi Eras, Bermúdez-Cardona, María Bianney, Murillo-Arango, Walter

“…Phytophthora cinnamomi causes one of the most important diseases in avocado crop and its chemical management represents 25% of the production cost per year…”
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Versatility of glutaraldehyde to immobilize lipases: Effect of the immobilization protocol on the properties of lipase B from Candida antarctica by Barbosa, Oveimar, Torres, Rodrigo, Ortiz, Claudia, Fernandez-Lafuente, Roberto

“…► CALB has been immobilized on amino and amino-glutaraldehyde activated supports. ► Different immobilization conditions have permitted to alter the…”
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Heterofunctional Supports in Enzyme Immobilization: From Traditional Immobilization Protocols to Opportunities in Tuning Enzyme Properties by Barbosa, Oveimar, Torres, Rodrigo, Ortiz, Claudia, Berenguer-Murcia, Ángel, Rodrigues, Rafael C, Fernandez-Lafuente, Roberto

“…A heterofunctional support for enzyme immobilization may be defined as that which possesses several distinct functionalities on its surface able to interact…”
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Importance of the Support Properties for Immobilization or Purification of Enzymes by Santos, Jose Cleiton S. dos, Barbosa, Oveimar, Ortiz, Claudia, Berenguer-Murcia, Angel, Rodrigues, Rafael C., Fernandez-Lafuente, Roberto

“…Immobilization and purification of enzymes are usual requirements for their industrial use. Both purification and immobilization have a common factor: they use…”
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Glyoxyl-ficin: An example where a more intense multipoint covalent attachment may decrease enzyme stability by Siar, El-Hocine, Morellon-Sterling, Roberto, Carballares, Diego, Rocha-Martin, Javier, Barbosa, Oveimar, Bolivar, Juan M., Fernandez-Lafuente, Roberto

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Stabilization of the hexameric glutamate dehydrogenase from Escherichia coli by cations and polyethyleneimine by Garcia-Galan, Cristina, Barbosa, Oveimar, Fernandez-Lafuente, Roberto

“…•GDH activity does not depend on the presence of cations, but its stability improves in the presence of Li+.•GDH is a hexameric enzyme, inactivation rate…”
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Stabilization of ficin extract by immobilization on glyoxyl agarose. Preliminary characterization of the biocatalyst performance in hydrolysis of proteins by Siar, El-Hocine, Zaak, Hadjer, Kornecki, Jakub F., Zidoune, Mohammed Nasreddine, Barbosa, Oveimar, Fernandez-Lafuente, Roberto

“…[Display omitted] •A protein extract containing Ficin was immobilized on glyoxyl agarose beads.•The activity was 60% using both BANA and casein as…”
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Solid phase chemical modification of agarose glyoxyl-ficin: Improving activity and stability properties by amination and modification with glutaraldehyde by Siar, El-Hocine, Arana-Peña, Sara, Barbosa, Oveimar, Zidoune, Mohammed Nasreddine, Fernandez-Lafuente, Roberto

“…[Display omitted] •Ficin extract has been immobilized on glyoxyl agarose.•Carbodiimide mediated amination of the immobilized enzyme altered stability and…”
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