First detection of mutated ERG11 gene in vulvovaginal Candida albicans isolates at Ouagadougou/Burkina Faso

First detection of mutated ERG11 gene in vulvovaginal Candida albicans isolates at Ouagadougou/Burkina Faso

Vulvovaginal candidiasis is an important cause of morbidity among women due to Candida species. In the last decades, resistance to azoles, first-line antifungals has increased. One molecular mechanism of azole resistance by Candida involves mutations in the ERG11 gene encoding lanosterol 14-[alpha]-...

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Published in:BMC infectious diseases Vol. 22; no. 1; pp. 1 - 678
Main Authors: Dovo, Essi Etonam, Zohoncon, Théodora Mahoukèdè, Tovo, Sessi Frida, Soubeiga, Serge Théophile, Kiendrebeogo, Isabelle Touwendpoulimdé, Yonli, Albert Théophane, Ouedraogo, Rogomenona Alice, Dabire, Amana Mètuor, Djigma, Florencia Wendkuuni, Nadembega, Christelle Wendyam, Belemgnegre, Marius, Ouedraogo, Paul, Obiri-Yeboah, Dorcas, Simpore, Jacques
Format: Journal Article
Language:English
Published: London BioMed Central Ltd 08-08-2022
BioMed Central
BMC
Subjects:
Analysis
Azole resistance
Azoles
Burkina Faso
Candida
Candida albicans
Candidiasis
Candidiasis, Vulvovaginal
Distribution
Drug resistance in microorganisms
Enzymes
ERG11 gene
Fluconazole
Forecasts and trends
Fungicides
Gene mutations
Genetic aspects
Health aspects
Heterocyclic compounds
Infectious diseases
Laboratories
Lanosterol
Morbidity
Mutation
Vagina
Vulvovaginitis
Women
Burkina Faso
France
Italy
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Summary:Vulvovaginal candidiasis is an important cause of morbidity among women due to Candida species. In the last decades, resistance to azoles, first-line antifungals has increased. One molecular mechanism of azole resistance by Candida involves mutations in the ERG11 gene encoding lanosterol 14-[alpha]-demethylase, the target enzyme. This study was conducted to identify the clinical Candida species associated in vulvovaginal candidiasis; to determine the rate of antifungal resistance among Candida albicans isolates and to determine mutated ERG11 gene at Saint Camille Hospital in Ouagadougou, Burkina Faso. Antifungals susceptibility were performed using Kirby-Bauer disk diffusion method. ERG11 gene was detected using conventional PCR in C. albicans isolates resistant to at least one azole. Out of 262 clinical strains isolated, C. albicans accounted for 59.90%, followed by Candida glabrata 27.86%, Candida famata 7.25%, Candida tropicalis 3.05% and Saccharomyces cerevisiae 1.91%. Resistance rate of fluconazole to C. albicans was 59.54%. ERG11 gene was found in 9.79% of 92 C. albicans strains resistant to azoles. This detection of mutated ERG11 gene in C. albicans is the first in Burkina Faso and may be a cause of azole resistance in recurrent Candida vulvovaginitis.
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ISSN:1471-2334
1471-2334
DOI:10.1186/s12879-022-07619-5