Serum microRNA-125b-5p expression in patients with dilated cardiomyopathy combined with heart failure and its effect on myocardial fibrosis

This paper was performed to decipher the serum microRNA (miR)-125b-5p expression in patients with dilated cardiomyopathy (DCM) combined with heart failure (HF) and its effect on myocardial fibrosis. Serum miR-125b-5p expression, LVEDD, LVESD, LVEF, LVFS, and NT-proBNP levels were evaluated in clinic...

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Bibliographic Details
Published in:	Scandinavian cardiovascular journal : SCJ Vol. 58; no. 1; p. 2373083
Main Authors:	Zhang, Yingjie, Deng, Daqing, Huang, Quan, Wu, Jiaru, Xiang, Yi, Ou, Boqing
Format:	Journal Article
Language:	English
Published:	England 31-12-2024
Subjects:	Adult Aged Animals Apoptosis Cardiomyopathy, Dilated - blood Cardiomyopathy, Dilated - genetics Cardiomyopathy, Dilated - pathology Case-Control Studies Circulating MicroRNA - blood Circulating MicroRNA - genetics Disease Models, Animal Female Fibrosis Heart Failure - blood Heart Failure - genetics Heart Failure - metabolism Heart Failure - pathology Humans Male MicroRNAs - blood MicroRNAs - genetics MicroRNAs - metabolism Middle Aged Myocardium - metabolism Myocardium - pathology Myocytes, Cardiac - metabolism Myocytes, Cardiac - pathology Natriuretic Peptide, Brain - blood Natriuretic Peptide, Brain - genetics Peptide Fragments - blood Rats, Sprague-Dawley Stroke Volume Ventricular Function, Left Ventricular Remodeling heart failure dilated cardiomyopathy microRNA-125b-5p myocardial fibrosis
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Summary:	This paper was performed to decipher the serum microRNA (miR)-125b-5p expression in patients with dilated cardiomyopathy (DCM) combined with heart failure (HF) and its effect on myocardial fibrosis. Serum miR-125b-5p expression, LVEDD, LVESD, LVEF, LVFS, and NT-proBNP levels were evaluated in clinical samples. A rat DCM model was established by continuous intraperitoneal injection of adriamycin and treated with miR-125b-5p agomir and its negative control. Cardiac function, serum TNF-α, hs-CRP, and NT-proBNP levels, pathological changes in myocardial tissues, cardiomyocyte apoptosis, and the expression levels of miR-125b-5p and fibrosis-related factors were detected in rats. In comparison to the control group, the case group had higher levels of LVEDD, LVESD, and NT-pro-BNP, and lower levels of LVEF, LVFS, and miR-125b-5p expression levels. Overexpression of miR-125b-5p effectively led to the improvement of cardiomyocyte hypertrophy and collagen arrangement disorder in DCM rats, the reduction of blue-stained collagen fibers in the interstitial myocardium, the reduction of the levels of TNF-α, hs-CRP, and NT-proBNP and the expression levels of TGF-1β, Collagen I, and α-SMA, and the reduction of the number of apoptosis in cardiomyocytes. Overexpression of miR-125b-5p is effective in ameliorating myocardial fibrosis.
ISSN:	1651-2006
DOI:	10.1080/14017431.2024.2373083