Search Results - "Otten, Harm"
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Insights into the oxidative degradation of cellulose by a copper metalloenzyme that exploits biomass components
Published in Proceedings of the National Academy of Sciences - PNAS (13-09-2011)“…The enzymatic degradation of recalcitrant plant biomass is one of the key industrial challenges of the 21st century. Accordingly, there is a continuing drive…”
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2
A new MR-SAD algorithm for the automatic building of protein models from low-resolution X-ray data and a poor starting model
Published in IUCrJ (01-03-2018)“…Determining macromolecular structures from X-ray data with resolution worse than 3 Å remains a challenge. Even if a related starting model is available, its…”
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Design of thermostable rhamnogalacturonan lyase mutants from Bacillus licheniformis by combination of targeted single point mutations
Published in Applied microbiology and biotechnology (01-05-2014)“…Rhamnogalacturonan I lyases (RGI lyases) (EC 4.2.2.-) catalyze cleavage of α-1,4 bonds between rhamnose and galacturonic acid in the backbone of pectins by…”
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Trauma team activation varies across Dutch emergency departments: a national survey
Published in Scandinavian journal of trauma, resuscitation and emergency medicine (16-11-2015)“…Tiered trauma team response may contribute to efficient in-hospital trauma triage by reducing the amount of resources required and by improving health…”
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5
Structural and Biochemical Studies Elucidate the Mechanism of Rhamnogalacturonan Lyase from Aspergillus aculeatus
Published in Journal of molecular biology (19-11-2010)“…We present here the first experimental evidence for bound substrate in the active site of a rhamnogalacturonan lyase belonging to family 4 of polysaccharide…”
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6
Influence of a Joining Helix on the BLUF Domain of the YcgF Photoreceptor from Escherichia coli
Published in Chembiochem : a European journal of chemical biology (13-10-2008)“…BLUF-domain-comprising photoreceptors sense blue light by utilizing FAD as a chromophore. The ycgF gene product of Escherichia coli is composed of a N-terminal…”
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A folded and immunogenic IgE-hyporeactive variant of the major allergen Phl p 1 produced in Escherichia coli
Published in BMC biotechnology (09-06-2015)“…Group 1 grass pollen allergens are a major cause of allergic disease. Specific immunotherapy involving controlled administration of allergens can be used as a…”
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L-glucitol oxidizing dehydrogenase from Bradyrhizobium japonicum USDA 110 for production of D-sorbose with enzymatic or electrochemical cofactor regeneration
Published in Applied microbiology and biotechnology (01-04-2014)“…A gene in Bradyrhizobium japonicum USDA 110, annotated as a ribitol dehydrogenase (RDH), had 87 % sequence identity (97 % positives) to the N-terminal 31 amino…”
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Structural characterization of the thermostable Bradyrhizobium japonicum D -sorbitol dehydrogenase
Published in Acta crystallographica. Section F, Structural biology communications (01-11-2016)“…Bradyrhizobium japonicum sorbitol dehydrogenase is NADH-dependent and is active at elevated temperatures. The best substrate is D-glucitol (a synonym for…”
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A new insight into the zinc-dependent DNA-cleavage by the colicin E7 nuclease: a crystallographic and computational studyElectronic supplementary information (ESI) available. See DOI: 10.1039/c4mt00195h
Published 22-10-2014“…The nuclease domain of colicin E7 metallonuclease (NColE7) contains its active centre at the C-terminus. The mutant ΔN4-NColE7-C* - where the four N-terminal…”
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11
Structural characterization of the thermostable Bradyrhizobium japonicumd-sorbitol dehydrogenase
Published in Acta crystallographica. Section F, Structural biology communications (01-11-2016)“…Bradyrhizobium japonicum sorbitol dehydrogenase is NADH‐dependent and is active at elevated temperatures. The best substrate is d‐glucitol (a synonym for…”
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12
The binding of zinc ions to Emericella nidulans endo-[beta]-1,4-galactanase is essential for crystal formation
Published in Acta crystallographica. Section F, Structural biology communications (01-08-2013)“…A novel Emericella nidulans endo-[beta]-1,4-galactanase (EnGAL) demonstrates a strong capacity to generate high levels of very potent prebiotic…”
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13
A new insight into the zinc-dependent DNA-cleavage by the colicin E7 nuclease: a crystallographic and computational study
Published in Metallomics (01-11-2014)“…The nuclease domain of colicin E7 metallonuclease (NColE7) contains its active centre at the C-terminus. The mutant ΔN4-NColE7-C* - where the four N-terminal…”
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14
The binding of zinc ions to Emericella nidulans endo-β-1,4-galactanase is essential for crystal formation
Published in Acta crystallographica. Section F, Structural biology and crystallization communications (01-08-2013)“…A novel Emericella nidulans endo‐β‐1,4‐galactanase (EnGAL) demonstrates a strong capacity to generate high levels of very potent prebiotic oligosaccharides…”
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15
Trauma team activation varies across Dutch emergency departments: a national survey
Published in Scandinavian journal of trauma, resuscitation and emergency medicine (2015)“…Inneholder sammendrag…”
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Crystallization and preliminary crystallographic analysis of an Escherichia coli-selected mutant of the nuclease domain of the metallonuclease colicin E7
Published in Acta crystallographica. Section F, Structural biology and crystallization communications (01-05-2013)“…The metallonuclease colicin E7 is a member of the HNH family of endonucleases. It serves as a bacterial toxin in Escherichia coli, protecting the host cell…”
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17
Substrate recognition and catalysis of polysaccharide lyases
Published in Acta crystallographica. Section A, Foundations of crystallography (16-08-2009)“…Abstract only…”
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