Proteomic analysis shows that stress response proteins are significantly up-regulated in resistant diploid wheat (Triticum monococcum) in response to attack by the grain aphid (Sitobion avenae)

Proteomic analysis shows that stress response proteins are significantly up-regulated in resistant diploid wheat (Triticum monococcum) in response to attack by the grain aphid (Sitobion avenae)

The grain aphid Sitobion avenae (F.) is a major pest of wheat, acting as a virus vector as well as causing direct plant damage. Commonly grown wheat varieties in the UK have only limited resistance to this pest. The present study was carried out to investigate the potential of a diploid wheat line (...

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Bibliographic Details
Published in:Molecular breeding Vol. 35; no. 2; pp. 57 - 22
Main Authors: Guan, Wenzhu, Ferry, Natalie, Edwards, Martin G, Bell, Howard A, Othman, Hamizah, Gatehouse, John A, Gatehouse, Angharad M. R
Format: Journal Article
Language:English
Published: Dordrecht Springer-Verlag 01-02-2015
Springer Netherlands
Springer Nature B.V
Subjects:
Aphididae
Aphidoidea
Biomedical and Life Sciences
Biotechnology
breeding
computer software
Database searching
Deoxyribonucleic acid
diploidy
DNA
DNA biosynthesis
DNA repair
Electrophoresis
Fecundity
Feeding
Gel electrophoresis
gene expression regulation
Gene regulation
genes
Grain
Growth rate
Infestation
leaf extracts
Life Sciences
longevity
Molecular biology
Oxidative stress
pest resistance
Pests
Photosynthesis
Plant biology
Plant breeding
plant damage
Plant extracts
Plant Genetics and Genomics
Plant Pathology
Plant Physiology
Plant Sciences
Proteins
Proteomics
Sitobion avenae
Stress response
Transcription
transcription (genetics)
Triticum aestivum
Triticum monococcum
two-dimensional gel electrophoresis
Viruses
Wheat
Oxidative stress
Biotic stress
Diploid wheat
Aphid resistance
Stress response
Wheat proteomics
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Summary:The grain aphid Sitobion avenae (F.) is a major pest of wheat, acting as a virus vector as well as causing direct plant damage. Commonly grown wheat varieties in the UK have only limited resistance to this pest. The present study was carried out to investigate the potential of a diploid wheat line (ACC20 PGR1755), reported as exhibiting resistance to S. avenae, to serve as a source of resistance genes. The diploid wheat line was confirmed as partially resistant, substantially reducing the fecundity, longevity and growth rate of the aphid. Proteomic analysis showed that approximately 200 protein spots were reproducibly detected in leaf extracts from both the resistant line and a comparable susceptible line (ACC5 PGR1735) using two-dimensional gel electrophoresis and image comparison software. Twenty-four spots were significantly up-regulated (>2-fold) in the resistant line after 24 h of aphid feeding (13 and 11 involved in local and systemic responses, respectively). Approximately 50 % of all differentially expressed protein spots were identified by a combination of database searching with MS and MS/MS data, revealing that the majority of proteins up-regulated by aphid infestation were involved in metabolic processes (including photosynthesis) and transcriptional regulation. However, in the resistant line only, several stress response proteins (including NBS–LRR-like proteins) and oxidative stress response proteins were identified as up-regulated in response to aphid feeding, as well as proteins involved in DNA synthesis/replication/repair. This study indicates that the resistant diploid line ACC20 PGR1755 may provide a valuable resource in breeding wheat for resistance to aphids.
Bibliography:http://dx.doi.org/10.1007/s11032-015-0220-x
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1380-3743
1572-9788
DOI:10.1007/s11032-015-0220-x