The requirement of the U12-type spliceosome in the development of Drosophila melanogaster: Insights from P-element mediated snRNA gene disruptions and the prospero pre -mRNA
A second class of pre-mRNA introns that require a spliceosome consisting of the U11, U12, U4atac/U6atac, U5 snRNPs for their removal has been identified in a wide range of taxa including plants, insects, and vertebrates. Such an intron, designated U12-type, has been identified in Drosophila melanoga...
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| Format: | Dissertation |
| Language: | English |
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ProQuest Dissertations & Theses
01-01-2000
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| Online Access: | Get full text |
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| Summary: | A second class of pre-mRNA introns that require a spliceosome consisting of the U11, U12, U4atac/U6atac, U5 snRNPs for their removal has been identified in a wide range of taxa including plants, insects, and vertebrates. Such an intron, designated U12-type, has been identified in Drosophila melanogaster prospero gene, which encodes a homeodomain protein required for central nervous system development (Doe et al., 1991). Its existence led me to characterize the single locus that specifies the U6atac snRNA of Drosophila and to identify a U4atac snRNA homolog. P-element mediated disruptions of the U6atac gene is lethal as a homozygote, with demonstrable defects in the excision of U12-type introns. Lethality can be rescued by introduction of a U6atac transgene. This is the first demonstration of a requirement of the U12-type spliceosome in the development of a metazoan organism. |
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| ISBN: | 9780493168005 0493168001 |