Search Results - "Osada, Toshiya"

Site-specific recombination of T2 phage using IP008 long tail fiber genes provides a targeted method for expanding host range while retaining lytic activity by Mahichi, Faezeh, Synnott, Aidan John, Yamamichi, Keiko, Osada, Toshiya, Tanji, Yasunori

“…The application of bacteriophages (phages) in therapy urgently requires the production of wide-host-range recombinant phages that possess strong lytic…”
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Exploring Transferrin-Receptor Interactions at the Single-Molecule Level by Yersin, Alexandre, Osada, Toshiya, Ikai, Atsushi

“…Interaction between the iron transporter protein transferrin (Tf) and its receptor at the cell surface is fundamental for most living organisms. Tf receptor…”
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Spectrin–ankyrin interaction mechanics: A key force balance factor in the red blood cell membrane skeleton by Saito, Masakazu, Watanabe-Nakayama, Takahiro, Machida, Shinichi, Osada, Toshiya, Afrin, Rehana, Ikai, Atsushi

“…As major components of red blood cell (RBC) cytoskeleton, spectrin and F-actin form a network that covers the entire cytoplasmic surface of the plasma…”
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Molecular shape and binding force of Mycoplasma mobile’s leg protein Gli349 revealed by an AFM study by Lesoil, Charles, Nonaka, Takahiro, Sekiguchi, Hiroshi, Osada, Toshiya, Miyata, Makoto, Afrin, Rehana, Ikai, Atsushi

“…Recent studies of the gliding bacteria Mycoplasma mobile have identified a family of proteins called the Gli family which was considered to be involved in this…”
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Nonlinear displacement of ventral stress fibers under externally applied lateral force by an atomic force microscope by Hakari, Tomoro, Sekiguchi, Hiroshi, Osada, Toshiya, Kishimoto, Kikuo, Afrin, Rehana, Ikai, Atsushi

“…Actin‐based stress fibers (SFs) have fundamental importance in the maintenance of mechanical stability of living cells. Several in vitro measurements of their…”
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Detection of mRNA in single living cells using AFM nanoprobes by Uehara, Hironori, Ikai, Atsushi, Osada, Toshiya

“…Examining messenger RNA (mRNA) expression is useful for the determination of cell and tissue conditions. Many methods of determining mRNA expression require…”
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Quantitative measurement of mRNA at different loci within an individual living cell by Uehara, Hironori, Osada, Toshiya, Ikai, Atsushi

“…Asymmetric localizations of cellular proteins and mRNAs are important for cell functions such as division, differentiation and development. The localization of…”
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Quantification of the number of EP3 receptors on a living CHO cell surface by the AFM by Kim, Hyonchol, Arakawa, Hideo, Hatae, Noriyuki, Sugimoto, Yukihiko, Matsumoto, Osamu, Osada, Toshiya, Ichikawa, Atsushi, Ikai, Atsushi

“…The distribution of EP3 receptors on a living cell surface was quantitatively studied by atomic force microscopy (AFM). Green fluorescent protein (GFP) was…”
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The Reporter System for GPCR Assay with the Fission Yeast Schizosaccharomyces pombe by Sasuga, Shintaro, Osada, Toshiya

“…G protein-coupled receptors (GPCRs) are associated with a great variety of biological activities. Yeasts are often utilized as a host for heterologous GPCR…”
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mRNA detection of individual cells with the single cell nanoprobe method compared with in situ hybridization by Uehara, Hironori, Kunitomi, Yuji, Ikai, Atsushi, Osada, Toshiya

“…The localization of specific mRNA generates cell polarity by controlling the translation sites of specific proteins. Although most of these events depend on…”
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High-sensitivity detection of proteins using gel electrophoresis and atomic force microscopy by Sekiguchi, Hiroshi, Hidaka, Atsushi, Shiga, Yoshihito, Ikai, Atsushi, Osada, Toshiya

“…We have developed a method to detect specific proteins with a high sensitivity using a gel electrophoresis method and force measurement of atomic force…”
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Quantification of fibronectin and cell surface interactions by AFM by Hyonchol, Kim, Arakawa, Hideo, Osada, Toshiya, Ikai, Atsushi

“…The cell adhesion strength between FN and the living cell was successfully examined using an AFM cantilever having a FN-coated spherical microbead as a probe…”
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mRNA analysis of single living cells by Osada, Toshiya, Uehara, Hironori, Kim, Hyonchol, Ikai, Atsushi

“…Analysis of specific gene expression in single living cells may become an important technique for cell biology. So far, no method has been available to detect…”
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Extraction of membrane proteins from a living cell surface using the atomic force microscope and covalent crosslinkers by Afrin, Rehana, Arakawa, Hideo, Osada, Toshiya, Ikai, Atsushi

“…The force curve mode of the atomic force microscope (AFM) was applied to extract intrinsic membrane proteins from the surface of live cells using AFM tips…”
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A comparative immunocytochemical study of development and regeneration of chemosensory neurons in the rat vomeronasal system by Matsuoka, Masato, Osada, Toshiya, Yoshida-Matsuoka, Junko, Ikai, Atsushi, Ichikawa, Masumi, Norita, Masao, Costanzo, Richard M

“…Vomeronasal neurons undergo continuous neurogenesis during development and after neuronal injury. We used immunocytochemical methods to compare different…”
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Tapping and contact mode imaging of native chromosomes and extraction of genomic DNA using AFM tips by Sun, Yingchun, Arakawa, Hideo, Osada, Toshiya, Ikai, Atsushi

“…It is very important both in medicine and biology to clarify the chromosomal structure to understand its functions. In a standard cytogenetic procedure,…”
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Bandeiraea simplicifolia lectin I and Vicia villosa agglutinin bind specifically to the vomeronasal axons in the accessory olfactory bulb of the rat by Ichikawa, M, Osada, T, Ikai, A

“…The binding of 21 lectins to the accessory olfactory bulb (AOB) of the rat was examined by histochemistry. Two lectins [Bandeiraea simplicifolia lectin I…”
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Differential binding patterns of three antibodies (VOBM1, VOBM2, and VOM2) in the rat vomeronasal organ and accessory olfactory bulb by Yoshida, J, Osada, T, Mori, Y, Ichikawa, M

“…Immunohistochemical properties of monoclonal antibodies raised against the rat vomeronasal epithelium were examined in adult rats. Three monoclonal antibodies,…”
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Vicia villosa agglutinin inhibits the fasciculation of vomeronasal axons in fetal rat vomeronasal organ culture by Ichikawa, M, Osada, T, Graziadei, P P

“…The vomeronasal organ of rat was cultured from embryonic 15-day littermates. During 4-8 days in vitro, vomeronasal axons originating from the explants of…”
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Quantification of cell adhesion force with AFM: distribution of vitronectin receptors on a living MC3T3-E1 cell by Kim, H., Arakawa, Hideo, Osada, Toshiya, Ikai, Atsushi

“…Distribution of vitronectin (VN) receptors on a living murine osteoblastic cell was successfully measured by atomic force microscopy (AFM). First, the…”
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