Primers for identification and polymorphism assessment of Vespertilionid bats in the Pacific Northwest

Primers for identification and polymorphism assessment of Vespertilionid bats in the Pacific Northwest

Species‐specific primers for portions of the mitochondrial DNA 16S ribosomal subunit gene were designed to identify bats of the family Vespertilionidae (Mammalia). Two fragments, each of 190 base pairs, were found to contain sufficient genetic variability to consistently resolve 10 of the 14 Pacific...

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Bibliographic Details
Published in:Molecular ecology notes Vol. 4; no. 2; pp. 239 - 242
Main Authors: Zinck, J.M, Duffield, D.A, Ormsbee, P.C
Format: Journal Article
Language:English
Published: Oxford, UK Blackwell Science Ltd 01-06-2004
Subjects:
16S ribosomal subunit gene
alleles
DNA primers
fecal DNA
genes
genetic markers
haplotypes
heterozygosity
loci
microsatellite repeats
mitochondrial DNA
mitochondrial DNA markers
molecular sequence data
noninvasive genetic sampling
nucleotide sequences
polymerase chain reaction
ribosomal RNA
Vespertilionidae
USA, Pacific Northwest
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Summary:Species‐specific primers for portions of the mitochondrial DNA 16S ribosomal subunit gene were designed to identify bats of the family Vespertilionidae (Mammalia). Two fragments, each of 190 base pairs, were found to contain sufficient genetic variability to consistently resolve 10 of the 14 Pacific Northwest species included in this study. The remaining four species could be resolved into two sets of paired species that were unique from the other 10 species. These primers are demonstrably useful for purposes of surveys designed for species identification of bats, including using DNA extracted from guano collected from roost sites when bats are absent.
Bibliography:http://onlinelibrary.wiley.com/journal/10.1111/(ISSN)1755-0998/issues
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ArticleID:MEN629
ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ISSN:1471-8278
1471-8286
DOI:10.1111/j.1471-8286.2004.00629.x