Search Results - "Orin, Shanks"

Quantitative CrAssphage PCR Assays for Human Fecal Pollution Measurement by Stachler, Elyse, Kelty, Catherine, Sivaganesan, Mano, Li, Xiang, Bibby, Kyle, Shanks, Orin C

“…Environmental waters are monitored for fecal pollution to protect public health and water resources. Traditionally, general fecal-indicator bacteria are used;…”
Get full text

Variability in RT-qPCR assay parameters indicates unreliable SARS-CoV-2 RNA quantification for wastewater surveillance by Bivins, Aaron, Kaya, Devrim, Bibby, Kyle, Simpson, Stuart L., Bustin, Stephen A., Shanks, Orin C., Ahmed, Warish

“…•Only 26% of published RT-qPCR assays used for wastewater surveillance reported all standard curve parameters.•Many of the reported standard curve parameters…”
Get full text

Genetic fecal source identification in urban streams impacted by municipal separate storm sewer system discharges by Diedrich, Adam, Sivaganesan, Mano, Willis, Jessica R, Sharifi, Amirreza, Shanks, Orin C

“…Municipal stormwater systems are designed to collect, transport, and discharge precipitation from a defined catchment area into local surface waters. However,…”
Get full text

A fecal score approximation model for analysis of real-time quantitative PCR fecal source identification measurements by Sivaganesan, Mano, Willis, Jessica R., Diedrich, Adam, Shanks, Orin C.

“…•Model useful for any qPCR applications yielding a large proportion of non-detections.•Description of novel fecal score approximation model for qPCR censored…”
Get full text

Variable fecal source prioritization in recreational waters routinely monitored with viral and bacterial general indicators by Li, Xiang, Kelty, Catherine A., Sivaganesan, Mano, Shanks, Orin C.

“…•Cooccurrence of coliphage, fecal indicator bacteria, and host-associated genetic markers•Use of different fecal indicators leads to prioritization of variable…”
Get full text

Fecal pollution source characterization at non-point source impacted beaches under dry and wet weather conditions by Shrestha, Abhilasha, Kelty, Catherine A., Sivaganesan, Mano, Shanks, Orin C., Dorevitch, Samuel

“…Though Lake Michigan beaches in Chicago are not impacted by stormwater or wastewater outfalls, several of those beaches often exceed USEPA Beach Action Values…”
Get full text

Quantitative fecal source characterization of urban municipal storm sewer system outfall ‘wet’ and ‘dry’ weather discharges by Shanks, Orin C., Diedrich, Adam, Sivaganesan, Mano, Willis, Jessica R., Sharifi, Amirreza

“…•Urban storm sewer system discharges frequently contained elevated E. coli levels.•Storm discharges consisted of human, avian, ruminant, and dog fecal…”
Get full text

Performance of forty-one microbial source tracking methods: A twenty-seven lab evaluation study by Boehm, Alexandria B., Van De Werfhorst, Laurie C., Griffith, John F., Holden, Patricia A., Jay, Jenny A., Shanks, Orin C., Wang, Dan, Weisberg, Stephen B.

“…The last decade has seen development of numerous new microbial source tracking (MST) methodologies, but many of these have been tested in just a few…”
Get full text

Large-scale implementation of standardized quantitative real-time PCR fecal source identification procedures in the Tillamook Bay Watershed by Li, Xiang, Sivaganesan, Mano, Kelty, Catherine A, Zimmer-Faust, Amity, Clinton, Pat, Reichman, Jay R, Johnson, York, Matthews, William, Bailey, Stephanie, Shanks, Orin C

“…Fecal pollution management remains one of the biggest challenges for water quality authorities worldwide. Advanced fecal pollution source identification…”
Get full text

Community Structures of Fecal Bacteria in Cattle from Different Animal Feeding Operations by SHANKS, Orin C, KELTY, Catherine A, ARCHIBEQUE, Shawn, JENKINS, Michael, NEWTON, Ryan J, MCLELLAN, Sandra L, HUSE, Susan M, SOGIN, Mitchell L

“…Classifications Services AEM Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley Reddit…”
Get full text

Improved HF183 quantitative real-time PCR assay for characterization of human fecal pollution in ambient surface water samples by Green, Hyatt C, Haugland, Richard A, Varma, Manju, Millen, Hana T, Borchardt, Mark A, Field, Katharine G, Walters, William A, Knight, R, Sivaganesan, Mano, Kelty, Catherine A, Shanks, Orin C

“…Quantitative real-time PCR (qPCR) assays that target the human-associated HF183 bacterial cluster within members of the genus Bacteroides are among the most…”
Get full text

Bacterial and viral fecal indicator predictive modeling at three Great Lakes recreational beach sites by Cyterski, Mike, Shanks, Orin C., Wanjugi, Pauline, McMinn, Brian, Korajkic, Asja, Oshima, Kevin, Haugland, Rich

“…•High variability in predictive performance was seen across the developed models using least-angle regression (LARS).•Enterococci and Bacteroidales qPCR models…”
Get full text

Contamination Scenario Matters when Using Viral and Bacterial Human-Associated Genetic Markers as Indicators of a Health Risk in Untreated Sewage-Impacted Recreational Waters by Schoen, Mary E, Boehm, Alexandria B, Soller, Jeffrey, Shanks, Orin C

“…Fecal pollution at beaches can pose a health risk to recreators. Quantitative microbial risk assessment (QMRA) is a tool to evaluate the use of candidate fecal…”
Get full text

Effectiveness of two wastewater disinfection strategies for the removal of fecal indicator bacteria, bacteriophage, and enteric viral pathogens concentrated using dead-end hollow fiber ultrafiltration (D-HFUF) by Korajkic, Asja, Kelleher, Julie, Shanks, Orin C., Herrmann, Michael P., McMinn, Brian R.

“…Primary influent and final effluent samples were collected from wastewater treatment plants using either chlorination or ultraviolet (UV) disinfection biweekly…”
Get full text

Comparison of the Microbial Community Structures of Untreated Wastewaters from Different Geographic Locales by SHANKS, Orin C, NEWTON, Ryan J, KELTY, Catherine A, HUSE, Susan M, SOGIN, Mitchell L, MCLELLAN, Sandra L

“…Classifications Services AEM Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley Reddit…”
Get full text

Extended persistence of general and cattle-associated fecal indicators in marine and freshwater environment by Korajkic, Asja, McMinn, Brian R., Ashbolt, Nicholas J., Sivaganesan, Mano, Harwood, Valerie J., Shanks, Orin C.

“…Fecal contamination of recreational waters with cattle manure can pose a risk to public health due to the potential presence of various zoonotic pathogens…”
Get full text

Performance of NIST SRM® 2917 with 13 recreational water quality monitoring qPCR assays by Willis, Jessica R., Sivaganesan, Mano, Haugland, Richard A., Kralj, Jason, Servetas, Stephanie, Hunter, Monique E., Jackson, Scott A., Shanks, Orin C.

“…•NIST SRM® 2917 functions with 13 qPCR assays to fecal waste in samples.•Yeast carrier tRNA stabilizer does not compromise qPCR performance.•All dilution…”
Get full text

Quantitative fecal pollution assessment with bacterial, viral, and molecular methods in small stream tributaries by McMinn, Brian R., Korajkic, Asja, Kelleher, Julie, Diedrich, Adam, Pemberton, Adin, Willis, Jessica R., Sivaganesan, Mano, Shireman, Brooke, Doyle, Andrew, Shanks, Orin C.

“…Stream water quality can be impacted by a myriad of fecal pollution sources and waste management practices. Identifying origins of fecal contamination can be…”
Get full text

Evaluation of genetic markers from the 16S rRNA gene V2 region for use in quantitative detection of selected Bacteroidales species and human fecal waste by qPCR by Haugland, Richard A., Varma, Manju, Sivaganesan, Mano, Kelty, Catherine, Peed, Lindsay, Shanks, Orin C.

“…Molecular methods for quantifying defined Bacteroidales species from the human gastrointestinal tract may have important clinical and environmental…”
Get full text

Viral and Bacterial Fecal Indicators in Untreated Wastewater across the Contiguous United States Exhibit Geospatial Trends by Korajkic, Asja, McMinn, Brian, Herrmann, Michael P, Sivaganesan, Mano, Kelty, Catherine A, Clinton, Pat, Nash, Maliha S, Shanks, Orin C

“…Cultivated fecal indicator bacteria such as and enterococci are typically used to assess the sanitary quality of recreational waters. However, these indicators…”
Get full text