Apolipoprotein L, a new human high density lipoprotein apolipoprotein expressed by the pancreas. Identification, cloning, characterization, and plasma distribution of apolipoprotein L

In this study, we have identified and characterized a new protein present in human high density lipoprotein that we have designated apolipoprotein L. Using a combination of liquid-phase isoelectrophoresis and high resolution two-dimensional gel electrophoresis, apolipoprotein L was identified and pa...

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Bibliographic Details
Published in:	The Journal of biological chemistry Vol. 272; no. 41; pp. 25576 - 25582
Main Authors:	Duchateau, P.N. (University of California, San Francisco), Pullinger, C.R, Orellana, R.E, Kunitake, S.T, Naya-Vigne, J, O'Connor, P.M, Malloy, M.J, Kane, J.P
Format:	Journal Article
Language:	English
Published:	United States 10-10-1997
Subjects:	ADN Amino Acid Sequence AMINO ACID SEQUENCES Apolipoprotein L1 Apolipoproteins - biosynthesis Apolipoproteins - genetics Apolipoproteins - isolation & purification APOPROTEINAS APOPROTEINE APOPROTEINS ARN MENSAJERO ARN MESSAGER Base Sequence BLOOD PLASMA Blotting, Northern CHEMICAL COMPOSITION Cloning, Molecular COMPLEMENTARY DNA COMPOSICION QUIMICA COMPOSITION CHIMIQUE DIAMETER DIAMETRE DIAMETRO DNA DNA, Complementary - analysis EXPRESION GENICA EXPRESSION DES GENES Female GENBANK/AF019225 GENE EXPRESSION GENERO HUMANO GENRE HUMAIN Humans Lipoprotein(a) - analogs & derivatives Lipoprotein(a) - chemistry LIPOPROTEINAS LIPOPROTEINE LIPOPROTEINS Lipoproteins, HDL - biosynthesis Lipoproteins, HDL - genetics Lipoproteins, HDL - isolation & purification Male MANKIND MESSENGER RNA MOLECULAR CONFORMATION MOLECULAR SEQUENCE DATA MOLECULAR WEIGHT NUCLEOTIDE SEQUENCE PANCREAS Pancreas - metabolism PESO MOLECULAR PLASMA SANGUIN PLASMA SANGUINEO POIDS MOLECULAIRE Protein Structure, Secondary PURIFICACION PURIFICATION SECUENCIA NUCLEOTIDICA SEQUENCE NUCLEOTIDIQUE
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Summary:	In this study, we have identified and characterized a new protein present in human high density lipoprotein that we have designated apolipoprotein L. Using a combination of liquid-phase isoelectrophoresis and high resolution two-dimensional gel electrophoresis, apolipoprotein L was identified and partially sequenced from immunoisolated high density lipoprotein (Lp(A-I)). Expression was only detected in the pancreas. The cDNA sequence encoding the full-length protein was cloned using reverse transcription-polymerase chain reaction. The deduced amino acid sequence contains 383 residues, including a typical signal peptide of 12 amino acids. No significant homology was found with known sequences. The plasma protein is a single chain polypeptide with an apparent molecular mass of 42 kDa. Antibodies raised against this protein detected a truncated form with a molecular mass of 39 kDa. Both forms were predominantly associated with immunoaffinity-isolated apoA-I-containing lipoproteins and detected mainly in the density range 1.123 < d < 1.21 g/ml. Free apoL was not detected in plasma. Anti-apoL immunoaffinity chromatography was used to purify apoL-containing lipoproteins (Lp(L)) directly from plasma. Nondenaturing gel electrophoresis of Lp(L) showed two major molecular species with apparent diameters of 12.2-17 and 10.4-12.2 nm. Moreover, Lp(L) exhibited both pre-beta and alpha electromobility. Apolipoproteins A-I, A-II, A-IV, and C-III were also detected in the apoL-containing lipoprotein particles.
Bibliography:	S20 1997090733 S30 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0021-9258 1083-351X
DOI:	10.1074/jbc.272.41.25576