Search Results - "Orbovic, Vladimir"

CRISPR‐LbCas12a‐mediated modification of citrus by Jia, Hongge, Orbović, Vladimir, Wang, Nian

“…Summary Recently, CRISPR‐Cas12a (Cpf1) from Prevotella and Francisella was engineered to modify plant genomes. In this report, we employed CRISPR‐LbCas12a…”
Get full text

Genome editing of the disease susceptibility gene CsLOB1 in citrus confers resistance to citrus canker by Jia, Hongge, Zhang, Yunzeng, Orbović, Vladimir, Xu, Jin, White, Frank F., Jones, Jeffrey B., Wang, Nian

“…Citrus is a highly valued tree crop worldwide, while, at the same time, citrus production faces many biotic challenges, including bacterial canker and…”
Get full text

A simple and efficient agroinfiltration method for transient gene expression in Citrus by Acanda, Yosvanis, Welker, Stacy, Orbović, Vladimir, Levy, Amit

“…Key message Microwounding pre-treatment facilitates agroinfiltration and transient gene expression in hard-to-agroinfiltrate citrus varieties. Agrobacterium…”
Get full text

Editorial: New Developments in Agrobacterium-Mediated Transformation of Tree Fruit Crops by Orbović, Vladimir

Get full text

Editorial: New developments in Agrobacterium Mediated Transformation of tree fruit crops, volume II by Orbovic, Vladimir, Prieto, Humberto

Get full text

Editing Citrus Genome via SaCas9/sgRNA System by Jia, Hongge, Xu, Jin, Orbović, Vladimir, Zhang, Yunzeng, Wang, Nian

“…SaCas9/sgRNA, derived from , is an alternative system for genome editing to SpCas9/sgRNA. The smaller SaCas9 recognizes a different protospacer adjacent motif…”
Get full text

Stress-inducible Arabidopsis thaliana RD29A promoter constitutively drives Citrus sinensis APETALA1 and LEAFY expression and precocious flowering in transgenic Citrus spp by Orbović, Vladimir, Ravanfar, Seyed Ali, Acanda, Yosvanis, Narvaez, Javier, Merritt, Benjamin A., Levy, Amit, Lovatt, Carol J.

“…Transgenic ‘Duncan’ grapefruit ( Citrus paradisi Macf.) and ‘Valencia’ sweet orange ( Citrus sinensis [L.] Osbeck) plants ectopically expressing C. sinensis…”
Get full text

Biallelic Editing of the LOB1 Promoter via CRISPR/Cas9 Creates Canker-Resistant 'Duncan' Grapefruit by Jia, Hongge, Omar, Ahmad A, Orbović, Vladimir, Wang, Nian

“…Citrus canker caused by subsp. is one of the most devastating citrus diseases worldwide. Generating disease-resistant citrus varieties is considered one of the…”
Get full text

Selection of transgenic citrus plants based on glyphosate tolerance conferred by a citrus 5-enolpyruvylshikimate-3-phosphate synthase variant by Merritt, Benjamin A., Zhang, Xudong, Triplett, Eric W., Mou, Zhonglin, Orbović, Vladimir

“…Key message We have defined the conditions for citrus transformations using glyphosate as selection agent. This protocol results in high transformation rate…”
Get full text

Agrobacterium -Mediated Transformation of Tree Fruit Crops: Methods, Progress, and Challenges by Song, Guo-Qing, Prieto, Humberto, Orbovic, Vladimir

“…Genetic engineering based on -mediated transformation has been a desirable tool to manipulate single or multiple genes of existing genotypes of woody fruit…”
Get full text

Multiplexed gene editing in citrus by using a multi-intron containing Cas9 gene by Sarkar, Poulami, Santiago Vazquez, Jorge, Zhou, Mingxi, Levy, Amit, Mou, Zhonglin, Orbović, Vladimir

“…Several expression systems have been developed in clustered regularly interspaced short palindromic repeats (CRISPR)-associated protein 9 (CRISPR/Cas9)…”
Get full text

Editorial: Innovative applications of sequencing technologies in plant science by Kalendar, Ruslan, Hunter, Charles, Orbovic, Vladimir

Get full text

Bacterial Pesticidal Protein Mpp51Aa1 Delivered via Transgenic Citrus Severely Impacts the Fecundity of Asian Citrus Psyllid, Diaphorina citri by Mishra, Ruchir, Narayana, Ravishankar, Ibanez, Freddy, Achor, Diann, Shilts, Turksen, El-Mohtar, Choaa, Orbović, Vladimir, Stelinski, Lukasz L, Bonning, Bryony C

“…The Asian citrus psyllid (ACP) Diaphorina citri vectors the causative agent of citrus greening disease that has the capacity to decimate citrus production. As…”
Get full text

Genetic Transformation of Commercially Important Mature Citrus Scions by Wu, Hao, Acanda, Yosvanis, Shankar, Alka, Peeples, Michael, Hubbard, Calvin, Orbovic′, Vladimir, Zale, Janice

“…ABSTRACT A Mature Citrus Facility (MCF) was established at the University of Florida's Citrus Research and Education Center (CREC) to produce transgenic citrus…”
Get full text

Editorial: Recent advances in plant genetic engineering and innovative applications by Kalendar, Ruslan, Orbovic, Vladimir, Egea-Cortines, Marcos, Song, Guo-qing

Get full text

A promoter trap in transgenic citrus mediates recognition of a broad spectrum of Xanthomonas citri pv. citri TALEs, including in planta-evolved derivatives by Shantharaj, Deepak, Minsavage, Gerald V, Orbović, Vladimir, Moore, Gloria A, Holmes, Danalyn R, Römer, Patrick, Horvath, Diana M, Lahaye, Thomas, Jones, Jeffrey B

“…Citrus bacterial canker (CBC), caused by Xanthomonas citri subsp. citri (Xcc), causes dramatic losses to the citrus industry worldwide. Transcription…”
Get full text

Citrus transformation using juvenile tissue explants by Orbović, Vladimir, Grosser, Jude W

“…The most frequently used method for production of citrus transgenic plants is via Agrobacterium-mediated transformation of tissues found on explants obtained…”
Get more information

Unstable Transgene Expression Affects Long-Term Efficacy of the Arabidopsis Immune Receptor EFR to Confer Quantitative Resistance to Citrus Canker Under Field Conditions by Jain, Mukesh, Minsavage, Gerald V., Thapa, Naweena, Horvath, Diana M., Zipfel, Cyril, Moore, Gloria A., Fisher, Latanya C., Orbović, Vladimir, Jones, Jeffrey B.

“…The Arabidopsis thaliana pattern recognition ELONGATION FACTOR-TU RECEPTOR (EFR) recognizes and binds the bacterial pathogen-associated molecular pattern…”
Get full text

New Somatic Hybrid Mandarin Tetraploids Generated by Optimized Protoplast Fusion and Confirmed by Molecular Marker Analysis and Flow Cytometry by Ćalović, Milica, Chen, Chunxian, Yu, Qibin, Orbović, Vladimir, Gmitter, Frederick G., Grosser, Jude W.

“…Six mandarin cultivars, Ponkan ( Citrus reticulata ), Willowleaf ( Citrus deliciosa ), Kinnow ( Citrus nobilis × C. deliciosa ), Murcott (purported C…”
Get full text

Production and characterization of transgenic Citrus plants carrying p35 anti-apoptotic gene by Orbović, Vladimir, Ćalović, Milica, Dutt, Manjul, Grosser, Jude W., Barthe, Gary

“…•Transgenic Citrus rootstock plants expressing anti-apoptotic gene were produced.•Transgenic plants maintained features of wild-type plants associated with…”
Get full text