Surgical stress influences cytokine content in autologous conditioned serum
REASONS FOR PERFORMING STUDY: No recommendations have been made regarding the relative timing of blood collection for autologous conditioned serum (ACS) preparation and surgical procedures. OBJECTIVES: 1) To identify effects of surgical stress on cytokine levels in ACS, 2) identify haematological ma...
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| Published in: | Equine veterinary journal Vol. 47; no. 2; pp. 212 - 217 |
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| Main Authors: | , , , , |
| Format: | Journal Article |
| Language: | English |
| Published: |
United States
British Equine Veterinary Association
01-03-2015
Blackwell Publishing Ltd Wiley Subscription Services, Inc |
| Subjects: | |
| Online Access: | Get full text |
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| Summary: | REASONS FOR PERFORMING STUDY: No recommendations have been made regarding the relative timing of blood collection for autologous conditioned serum (ACS) preparation and surgical procedures. OBJECTIVES: 1) To identify effects of surgical stress on cytokine levels in ACS, 2) identify haematological markers for prediction of cytokine production in ACS and 3) investigate the necessity for specialised ACS containers when preparing a cytokine‐rich serum. STUDY DESIGN: Experimental in vitro study. METHODS: Blood was drawn from 15 stallions admitted for elective castration preoperatively and 22–24 h post operatively and incubated in ACS containers and plastic vacutainer tubes containing Z Serum Clot Activator. Concentrations of interleukin (IL)‐1 receptor agonist (IL‐1Ra), IL‐10, IL‐1β, tumour necrosis factor (TNF)‐α, insulin‐like growth factor (IGF)‐1 and transforming growth factor (TGF)‐β were determined in all serum samples and compared between preparation methods and sampling time by ANOVA. Changes in cytokine levels induced by incubation, defined as delta cytokine, were calculated by subtracting the baseline levels from the levels in incubated samples. Based on post operative serum amyloid A (SAA), horses were grouped into ‘mild‘, moderate’ and ‘marked’ surgical stress; delta cytokine levels in post operative samples were compared between these groups by ANOVA. RESULTS: Delta IGF‐1 was significantly lower in post operative samples compared with preoperative. Horses in the ‘marked’ surgical stress group had significantly lower delta IL‐1Ra and delta TGF‐β than the ‘moderate’ group and significantly lower delta IGF‐1 than the ‘mild’ group. No association between cytokine levels and haematology variables were identified. Cytokine levels were comparable between serum prepared in blood tubes and in specialised ACS containers. CONCLUSIONS: Surgical stress influences the cytokine content in ACS. Useful predictors of cytokine production in ACS were not identified. Specialised ACS containers may not be necessary for preparation of a cytokine‐rich serum. |
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| Bibliography: | http://dx.doi.org/10.1111/evj.12277 istex:85FBC07BD9F8C80B73C464E8BBA927B1317B57A6 ark:/67375/WNG-N4PJ0KP0-D Swedish/Norwegian Foundation for Equine Research ArticleID:EVJ12277 VetLine Bio AB Norwegian School of Veterinary Science ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
| ISSN: | 0425-1644 2042-3306 |
| DOI: | 10.1111/evj.12277 |