Electrochemical sensor based on phospholipid modified glassy carbon electrode - determination of paraquat

Electrochemical sensor based on phospholipid modified glassy carbon electrode - determination of paraquat

Electrochemical sensor based on glassy carbon electrode modified with asolectin (mixture of soybean phospholipids) was developed. Properties of phospholipid layer, such as thickness and compactness, were investigated using atomic force microscopy and X-ray photoelectron spectroscopy. Phospholipids c...

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Bibliographic Details
Published in:Journal of electroanalytical chemistry (Lausanne, Switzerland) Vol. 821; pp. 33 - 39
Main Authors: Tomková, H., Sokolová, R., Opletal, T., Kučerová, P., Kučera, L., Součková, J., Skopalová, J., Barták, P.
Format: Journal Article
Language:English
Published: Amsterdam Elsevier B.V 15-07-2018
Elsevier Science Ltd
Subjects:
Asolectin
Atomic force microscopy
Cell membranes
Cellular biology
Chemical sensors
Dependence
Electrodes
Glassy carbon
High performance liquid chromatography
Linearity
Mercury (metal)
NMR
Nuclear magnetic resonance
Paraquat
Phospholipid
Phospholipids
Sensor
Soybeans
Square wave voltammetry
Square waves
Thickness
Voltammetry
Asolectin
Phospholipid
Paraquat
Square wave voltammetry
Sensor
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Summary:Electrochemical sensor based on glassy carbon electrode modified with asolectin (mixture of soybean phospholipids) was developed. Properties of phospholipid layer, such as thickness and compactness, were investigated using atomic force microscopy and X-ray photoelectron spectroscopy. Phospholipids completely cover whole surface and the thickness of the layer 7.1±1.2nm corresponds to the value of the phospholipid bilayer of cell membranes. Due to the accumulation of paraquat in the phospholipid layer, square wave voltammetry with asolectin modified electrode exhibits three times higher sensitivity, measured as a slope of calibration dependence, and better linearity in comparison to unmodified glassy carbon electrode. Limit of detection 2.2nmolL−1 and recoveries higher than 88% were achieved in model samples of water. The method was used for determination of paraquat in real sample of river water and results were compared with high performance liquid chromatography and differential pulse voltammetry on hanging mercury drop electrode giving 0.071±0.010, 0.050±0.010 and 0.061±0.008μmolL−1, respectively. [Display omitted] •Low-cost electrochemical sensor for determination of paraquat was developed.•Mixture of soybean phospholipids improves sensitivity on nanomolar level.•AFM and XPS were used to characterize the compact phospholipid layer.•Thickness 7.1nm corresponds to phospholipid bilayers of the cell membranes.
ISSN:1572-6657
1873-2569
DOI:10.1016/j.jelechem.2017.12.048