Search Results - "Ooi, Toshihiko"

Synergy of valine and threonine supplementation on poly(2-hydroxybutyrate-block-3-hydroxybutyrate) synthesis in engineered Escherichia coli expressing chimeric polyhydroxyalkanoate synthase by Sudo, Maho, Hori, Chiaki, Ooi, Toshihiko, Mizuno, Shoji, Tsuge, Takeharu, Matsumoto, Ken'ichiro

“…The engineered chimeric polyhydroxyalkanoate (PHA) synthase PhaCAR is composed of N-terminal portion of Aeromonas caviae PHA synthase and C-terminal portion of…”
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In Vitro Analysis of d‑Lactyl-CoA-Polymerizing Polyhydroxyalkanoate Synthase in Polylactate and Poly(lactate-co-3-hydroxybutyrate) Syntheses by Matsumoto, Ken’ichiro, Iijima, Midori, Hori, Chiaki, Utsunomia, Camila, Ooi, Toshihiko, Taguchi, Seiichi

“…Engineered d-lactyl-coenzyme A (LA-CoA)-polymerizing polyhydroxyalkanoate synthase (PhaC1PsSTQK) efficiently produces poly­(lactate-co-3-hydroxybutyrate)…”
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Ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO)-mediated de novo synthesis of glycolate-based polyhydroxyalkanoate in Escherichia coli by Matsumoto, Ken'ichiro, Saito, Juri, Yokoo, Toshinori, Hori, Chiaki, Nagata, Akihiro, Kudoh, Yuki, Ooi, Toshihiko, Taguchi, Seiichi

“…Ribulose-1,5-bisphosphate (RuBP) carboxylase/oxygenase (RuBisCO) generates 2-phosphoglycolate (2PG) as one of the metabolites from the Calvin-Benson-Bassham…”
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Enhanced production of lactate-based polyesters in Escherichia coli from a mixture of glucose and xylose by Mlc-mediated catabolite derepression by Kadoya, Ryosuke, Matsumoto, Ken'ichiro, Takisawa, Kenji, Ooi, Toshihiko, Taguchi, Seiichi

“…Lignocellulose-utilizing biorefinery is a promising strategy for the sustainable production of value-added products such as bio-based polymers. Simultaneous…”
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Site-directed saturation mutagenesis of polyhydroxylalkanoate synthase for efficient microbial production of poly[(R)-2-hydroxybutyrate] by Hori, Chiaki, Oishi, Kenta, Matsumoto, Ken'ichiro, Taguchi, Seiichi, Ooi, Toshihiko

“…In our previous study, artificial polyhydroxyalkanoate (PHA) poly[(R)-2-hydroxybutyrate] [P(2HB)] was successfully biosynthesized from racemic 2HB in…”
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High-cell density culture of poly(lactate-co-3-hydroxybutyrate)-producing Escherichia coli by using glucose/xylose-switching fed-batch jar fermentation by Hori, Chiaki, Yamazaki, Takashi, Ribordy, Greg, Takisawa, Kenji, Matsumoto, Ken'ichiro, Ooi, Toshihiko, Zinn, Manfred, Taguchi, Seiichi

“…Poly(lactate-co-3-hydroxybutyrate) [P(LA-co-3HB)] is produced in engineered Escherichia coli harboring the genes encoding an LA-polymerizing enzyme (LPE) and…”
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Xylose-based hydrolysate from eucalyptus extract as feedstock for poly(lactate-co-3-hydroxybutyrate) production in engineered Escherichia coli by Takisawa, Kenji, Ooi, Toshihiko, Matsumoto, Ken’ichiro, Kadoya, Ryosuke, Taguchi, Seiichi

“…[Display omitted] •Hydrolysate from woody extract was useful as a feedstock for P(LA-co-3HB) production.•Xylose-based sugar mixture from the hydrolysate was…”
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MtgA Deletion-Triggered Cell Enlargement of Escherichia coli for Enhanced Intracellular Polyester Accumulation by Kadoya, Ryosuke, Matsumoto, Ken'ichiro, Ooi, Toshihiko, Taguchi, Seiichi

“…Bacterial polyester polyhydroxyalkanoates (PHAs) have been produced in engineered Escherichia coli, which turned into an efficient and versatile platform by…”
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Effect of monomeric composition on the thermal, mechanical and crystalline properties of poly[(R)-lactate-co-(R)-3-hydroxybutyrate] by Ishii, Daisuke, Takisawa, Kenji, Matsumoto, Ken'ichiro, Ooi, Toshihiko, Hikima, Takaaki, Takata, Masaki, Taguchi, Seiichi, Iwata, Tadahisa

“…Poly[(R)-lactate-co-(R)-3-hydroxybutyrate] [P(LA-co-3HB)] is a biobased polyester with flexible properties efficiently synthesized by engineered Escherichia…”
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Efficient (R)-3-hydroxybutyrate production using acetyl CoA-regenerating pathway catalyzed by coenzyme A transferase by Matsumoto, Ken’ichiro, Okei, Takehiro, Honma, Inori, Ooi, Toshihiko, Aoki, Hirobumi, Taguchi, Seiichi

“…( R )-3-hydroxybutyrate [( R )-3HB] is a useful precursor in the synthesis of value-added chiral compounds such as antibiotics and vitamins. Typically, ( R…”
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Dynamic Changes of Intracellular Monomer Levels Regulate Block Sequence of Polyhydroxyalkanoates in Engineered Escherichia coli by Matsumoto, Ken’ichiro, Hori, Chiaki, Fujii, Ryunosuke, Takaya, Masahiro, Ooba, Takashi, Ooi, Toshihiko, Isono, Takuya, Satoh, Toshifumi, Taguchi, Seiichi

“…Biological polymer synthetic systems, which utilize no template molecules, normally synthesize random copolymers. We report an exception, a synthesis of block…”
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Enhanced production of poly(lactate-co-3-hydroxybutyrate) from xylose in engineered Escherichia coli overexpressing a galactitol transporter by Nduko, John Masani, Matsumoto, Ken’ichiro, Ooi, Toshihiko, Taguchi, Seiichi

“…Poly(lactate-co-3-hydroxybutyrate) (P(LA-co-3HB)) was previously produced from xylose in engineered Escherichia coli. The aim of this study was to increase the…”
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Effectiveness of xylose utilization for high yield production of lactate-enriched P(lactate-co-3-hydroxybutyrate) using a lactate-overproducing strain of Escherichia coli and an evolved lactate-polymerizing enzyme by Nduko, John Masani, Matsumoto, Ken’ichiro, Ooi, Toshihiko, Taguchi, Seiichi

“…Xylose, which is a major constituent of lignocellulosic biomass, was utilized for the production of poly(lactate-co-3-hydroxybutyrate) [P(LA-co-3HB)], having…”
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Genome-wide screening of transcription factor deletion targets in Escherichia coli for enhanced production of lactate-based polyesters by Kadoya, Ryosuke, Kodama, Yu, Matsumoto, Ken'ichiro, Ooi, Toshihiko, Taguchi, Seiichi

“…Engineered Escherichia coli is a useful platform for production of lactate (LA)-based polyester poly[LA-co-3-hydroxybutyrate (3HB)] from renewable sugars. Here…”
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Molecular weight-dependent degradation of D-lactate-containing polyesters by polyhydroxyalkanoate depolymerases from Variovorax sp. C34 and Alcaligenes faecalis T1 by Sun, Jian, Matsumoto, Ken’ichiro, Tabata, Yuta, Kadoya, Ryosuke, Ooi, Toshihiko, Abe, Hideki, Taguchi, Seiichi

“…Polyhydroxyalkanoate depolymerase derived from Variovorax sp. C34 (PhaZVₛ) was identified as the first enzyme that is capable of degrading isotactic P[67 mol%…”
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Biosynthesis of Random-Homo Block Copolymer Poly[Glycolate- ran -3-Hydroxybutyrate (3HB)]- b -Poly(3HB) Using Sequence-Regulating Chimeric Polyhydroxyalkanoate Synthase in Escherichia coli by Arai, Shuzo, Sakakibara, Sayaka, Mareschal, Robin, Ooi, Toshihiko, Zinn, Manfred, Matsumoto, Ken'ichiro

“…Glycolate (GL)-containing polyhydroxyalkanoate (PHA) was synthesized in expressing the engineered chimeric PHA synthase PhaC and coenzyme A transferase. The…”
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Microbial production of poly(lactate-co-3-hydroxybutyrate) from hybrid Miscanthus-derived sugars by Sun, Jian, Utsunomia, Camila, Sasaki, Shohei, Matsumoto, Ken'ichiro, Yamada, Toshihiko, Ooi, Toshihiko, Taguchi, Seiichi

“…P[(R)-lactate-co-(R)-3-hydroxybutyrate] [P(LA-co-3HB)] was produced in engineered Escherichia coli using lignocellulose-derived hydrolysates from…”
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Characterization of thermostable FMN-dependent NADH azoreductase from the moderate thermophile Geobacillus stearothermophilus by Matsumoto, Ken'ichiro, Mukai, Yuichi, Ogata, Daiki, Shozui, Fumi, Nduko, John Masani, Taguchi, Seiichi, Ooi, Toshihiko

“…The gene encoding an FMN-dependent NADH azoreductase, AzrG, from thermophilic Geobacillus stearothermophilus was cloned and functionally expressed in…”
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Erratum to: Efficient (R)-3-hydroxybutyrate production using acetyl CoA-regenerating pathway catalyzed by coenzyme A transferase by Matsumoto, Ken’ichiro, Okei, Takehiro, Honma, Inori, Ooi, Toshihiko, Aoki, Hirobumi, Taguchi, Seiichi

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Comparative enzymatic analysis of azoreductases from Bacillus sp. B29 by Ooi, T.(Hokkaido Univ., Sapporo (Japan)), Shibata, T, Matsumoto, K, Kinoshita, S, Taguchi, S

“…We cloned and expressed two genes encoding azoreductase homologes, AzrB and AzrC, from Bacillus sp. B29. Purified recombinant AzrB and AzrC were homodimers…”
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