Optimisation of Electroporation for Biochemical Experiments in Live Cells

Optimisation of Electroporation for Biochemical Experiments in Live Cells

To introduce into cells small molecules, which do not permeate the cell membrane naturally, electroporation is the fastest and most efficient technique. Although it is not completely benign, the speed at which a full population of cells can be permeated gives it a strong advantage over all other cel...

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Bibliographic Details
Published in:Biochemical and biophysical research communications Vol. 256; no. 1; pp. 235 - 239
Main Authors: Meldrum, Rosalind A., Bowl, Michael, Ong, Swee Bee, Richardson, Simon
Format: Journal Article
Language:English
Published: United States Elsevier Inc 05-03-1999
Subjects:
Annexin A5 - metabolism
Ascorbic Acid - pharmacology
Calcium - metabolism
Cell Death
Cell Membrane Permeability
Cell Survival - drug effects
Cells, Cultured
Egtazic Acid - pharmacology
Electroporation - methods
Fluoresceins - metabolism
Fluorescent Dyes - metabolism
HL-60 Cells
Humans
Intercalating Agents - metabolism
Lipid Peroxidation
Phosphatidylserines - metabolism
Propidium - metabolism
Reactive Oxygen Species - metabolism
Time Factors
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Summary:To introduce into cells small molecules, which do not permeate the cell membrane naturally, electroporation is the fastest and most efficient technique. Although it is not completely benign, the speed at which a full population of cells can be permeated gives it a strong advantage over all other cell permeation techniques. Here we describe the potential damaging effects of electroporation and how to derive conditions which avoid these and assure its use for biochemical experiments in live cells.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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content type line 23
ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.1999.0246