A polysaccharide fraction from Achillea millefolium increases cytokine secretion and reduces activation of Akt, ERK and NF-κB in THP-1 monocytes

A polysaccharide fraction from Achillea millefolium increases cytokine secretion and reduces activation of Akt, ERK and NF-κB in THP-1 monocytes

•A polysaccharide, Am-25-d, was isolated from Achillea millefolium.•Am-25-d increased LPS-induced cytokine secretion by THP-1 monocytes.•Am-25-d decreased LPS-induced phosphorylation/activation of Akt, ERK1/2 and NF-κB.•Am-25-d may be of use as an immunoenhancer. Achillea millefolium has been used i...

Full description

Saved in:
Bibliographic Details
Published in:Carbohydrate polymers Vol. 143; pp. 131 - 138
Main Authors: Freysdottir, Jona, Logadottir, Oddny T., Omarsdottir, Sesselja S., Vikingsson, Arnor, Hardardottir, Ingibjorg
Format: Journal Article
Language:English
Published: England Elsevier Ltd 05-06-2016
Subjects:
Achillea - chemistry
Achillea millefolium
Cell Line, Tumor
Cytokines
Cytokines - metabolism
Humans
Immunologic Factors - chemistry
Immunologic Factors - isolation & purification
Immunologic Factors - pharmacology
Interleukin-10 - metabolism
Interleukin-12 Subunit p40 - metabolism
MAP kinases
Mitogen-Activated Protein Kinase 1 - metabolism
Mitogen-Activated Protein Kinase 3 - metabolism
NF-kappa B - metabolism
Polysaccharide
Polysaccharides - chemistry
Polysaccharides - isolation & purification
Polysaccharides - pharmacology
Proto-Oncogene Proteins c-akt - metabolism
Signal Transduction - drug effects
THP-1 cells
Tumor Necrosis Factor-alpha - metabolism
IL
Cytokines
THP-1 cells
TNF
LPS
TFA
IFN
MFI
PMP
DCs
RG
SI
Achillea millefolium
MAP kinases
PI
SEM
TLR
Polysaccharide
ELISA
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:•A polysaccharide, Am-25-d, was isolated from Achillea millefolium.•Am-25-d increased LPS-induced cytokine secretion by THP-1 monocytes.•Am-25-d decreased LPS-induced phosphorylation/activation of Akt, ERK1/2 and NF-κB.•Am-25-d may be of use as an immunoenhancer. Achillea millefolium has been used in traditional medicine for a number of ailments, including skin inflammation and wounds. A polysaccharide fraction (Am-25-d) isolated from aqueous extract from A. millefolium had an average molecular weight of 270kDa and a monosaccharide composition of GalA, Gal, Ara, Xyl, Rha in molar ratio of 28:26:23:9:7. THP-1 cells primed with IFN-γ and stimulated with LPS in the presence of Am-25-d secreted more IL-1β, IL-8, IL-10, IL-12p40, IL-23 and TNF-α than THP-1 cells stimulated in the absence of Am-25-d. However, when added to unstimulated cells Am-25-d did not increase secretion of the cytokines examined. Stimulating THP-1 monocytes in the presence of Am-25-d led to decreased nuclear concentrations of NF-κB and phosphorylation of ERK1/2 and Akt kinases compared with that when the cells were stimulated without Am-25-d. These findings indicate that Am-25-d isolated from A. millefolium has immunoenhancing properties that may be mediated via the Akt pathway.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0144-8617
1879-1344
DOI:10.1016/j.carbpol.2016.02.017