Molecular Typing of Methicillin Resistant Staphylococcus aureus Clinical Isolates on the Basis of Protein A and Coagulase Gene Polymorphisms

Increased frequency of methicillin-resistant Staphylococcus aureus (MRSA) in hospitalized patients requires rapid and reliable characterization of isolates for control of MRSA spread in hospitals. This study evaluated polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) as a...

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Bibliographic Details
Published in:	International Journal of Microbiology Vol. 2014; no. 2014; pp. 290 - 300
Main Authors:	Omar, Nancy Younis, Ali, Hala Abdel Salam, Harfoush, Reem Abdel Hameed, El Khayat, Engy Hamdy
Format:	Journal Article
Language:	English
Published:	Cairo, Egypt Hindawi Limiteds 2014 Hindawi Puplishing Corporation Hindawi Publishing Corporation John Wiley & Sons, Inc Hindawi Limited
Subjects:	Antibiotics Enzymes Gene amplification Genetic aspects Genetic polymorphisms Health aspects Hospitals Identification and classification Methods Microbiology Proteins Staphylococcus aureus Staphylococcus infections Surveillance
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Summary:	Increased frequency of methicillin-resistant Staphylococcus aureus (MRSA) in hospitalized patients requires rapid and reliable characterization of isolates for control of MRSA spread in hospitals. This study evaluated polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) as a molecular typing technique for MRSA strains on the basis of protein A (spa) and coagulase (coa) gene polymorphisms to verify their ability in assessing the relatedness of isolates. Seventy-five MRSA isolates, from different ICUs of Alexandria University Main Hospital, were characterized using antibiotyping and PCR-RFLP analysis of coa and spa genes. Thirty-two antibiotypes were identified. coa gene PCR generated 3 types and 10 subtypes of band patterns. HaeIII restriction digestion of amplified coa gene products produced 5 major banding patterns and 12 subtypes. spa gene PCR products generated 4 major and 11 minor types, and their HaeII restriction digestion showed 5 major and 12 minor banding patterns. The combined coa and spa RFLP patterns generated 22 combined R types. Typing using coa PCR and PCR-RFLP had the same discriminatory index (DI) value (0.64), which was comparable to that of both spa PCR and PCR-RFLP techniques (0.68). The combined grouping increased the DI value to 0.836. The current study revealed that testing for multiple gene polymorphisms is more useful for local epidemiologic purposes.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Academic Editor: Joseph Falkinham
ISSN:	1687-918X 1687-9198
DOI:	10.1155/2014/650328