ENDOXY - Development of a Biomimetic Oxygenator-Test-Device

This study focusses on the development of a biomimetic oxygenator test device. Due to limited biocompatibility, current oxygenators do not allow mid- to long-term therapy. Tissue engineering uses autologous cell sources to overcome the immunogenic barriers of biomaterials. Surface coating with endot...

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Bibliographic Details
Published in:	PloS one Vol. 10; no. 12; p. e0142961
Main Authors:	Dietrich, Maren, Finocchiaro, Nicole, Olszweski, Sebastian, Arens, Jutta, Schmitz-Rode, Thomas, Sachweh, Joerg, Jockenhoevel, Stefan, Cornelissen, Christian G
Format:	Journal Article
Language:	English
Published:	United States Public Library of Science 18-12-2015 Public Library of Science (PLoS)
Subjects:	Biocompatibility Biomaterials Biomedical materials Biomimetic Materials Biomimetics Blood Cell culture Endothelial cells Endothelial Cells - cytology Endothelium Equipment Design Gene expression Immunogenicity Materials Testing - instrumentation Materials Testing - methods Medical equipment Membranes Morphology Nitric oxide Oxygenators Oxygenators, Membrane Permeability Physical characteristics Retention Shear stress Shear stresses Stress, Mechanical Surgical apparatus & instruments Systems design Test procedures Tissue engineering Tissue Engineering - instrumentation Germany Aachen Germany
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Summary:	This study focusses on the development of a biomimetic oxygenator test device. Due to limited biocompatibility, current oxygenators do not allow mid- to long-term therapy. Tissue engineering uses autologous cell sources to overcome the immunogenic barriers of biomaterials. Surface coating with endothelial cells might improve hemocompatibility and thus prevent immunogenic reactions of the body. In this study this concept is applied to endothelialise a gas-permeable membrane to develop a biomimetic oxygenator test-device (ENDOXY). ENDOXY-a multifunctional test-system was developed to endothelialise a gas-permeable membrane suitable for cell culture and to test the cell retention under shear stress and to measure gas transfer through it. Successful endothelialisation of the membrane was achieved and cells showed characteristic endothelial morphologies. They stained positive for endothelial markers. The number of cells aligned with shear stress and cell retention after blood perfusing experiments was high. Gas transfer is observed via uncoated and endothelialised membranes. The study showed promising results with regard to system design, endothelialisation, and cell retention under shear stress conditions. It strongly encourages further research into the system by testing different membrane materials to design a biomimetic membrane surface and pave way for a fully hemocompatible oxygenator.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Competing Interests: The authors have declared that no competing interests exist. Conceived and designed the experiments: MD JA TSR SJ CGC NF. Performed the experiments: MD SO CGC. Analyzed the data: MD CGC NF. Contributed reagents/materials/analysis tools: JA TSR SJ. Wrote the paper: MD JA JS CGC.
ISSN:	1932-6203 1932-6203
DOI:	10.1371/journal.pone.0142961