Bioinformatics Designing and Molecular Modelling of a Universal mRNA Vaccine for SARS-CoV-2 Infection

At this present stage of COVID-19 re-emergence, designing an effective candidate vaccine for different variants of SARS-CoV-2 is a study worthy of consideration. This research used bioinformatics tools to design an mRNA vaccine that captures all the circulating variants and lineages of the virus in...

Full description

Saved in:

Bibliographic Details
Published in:	Vaccines (Basel) Vol. 10; no. 12; p. 2107
Main Authors:	Oladipo, Elijah Kolawole, Adeniyi, Micheal Oluwafemi, Ogunlowo, Mercy Temiloluwa, Irewolede, Boluwatife Ayobami, Adekanola, Victoria Oluwapelumi, Oluseyi, Glory Samuel, Omilola, Janet Abisola, Udoh, Anietie Femi, Olufemi, Seun Elijah, Adediran, Daniel Adewole, Olonade, Aanuoluwapo, Idowu, Usman Abiodun, Kolawole, Olatunji M, Oloke, Julius Kola, Onyeaka, Helen
Format:	Journal Article
Language:	English
Published:	Switzerland MDPI AG 01-12-2022 MDPI
Subjects:	Amino acids Antigens Bioinformatics CD4 antigen CD8 antigen Computational biology COVID-19 Cytotoxicity Deformability Epitopes Formability Genomes Immune response (cell-mediated) Immune system Immunological memory In vitro methods and tests In vivo methods and tests lineages Lymphocytes Lymphocytes B Lymphocytes T Macrophages Memory cells Methods Molecular modelling Molecular weight Molecules mRNA mRNA vaccine mRNA vaccines Natural killer cells Pandemics Peptides Proteins SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 Simulation Vaccines variants Viral diseases World population Nigeria SARS-CoV-2 variants bioinformatics lineages mRNA vaccine
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	At this present stage of COVID-19 re-emergence, designing an effective candidate vaccine for different variants of SARS-CoV-2 is a study worthy of consideration. This research used bioinformatics tools to design an mRNA vaccine that captures all the circulating variants and lineages of the virus in its construct. Sequences of these viruses were retrieved across the six continents and analyzed using different tools to screen for the preferable CD8 T lymphocytes (CTL), CD4 T lymphocytes (HTL), and B-cell epitopes. These epitopes were used to design the vaccine. In addition, several other co-translational residues were added to the construct of an mRNA vaccine whose molecular weight is 285.29686 kDa with an estimated pI of 9.2 and has no cross affinity with the human genome with an estimated over 68% to cover the world population. It is relatively stable, with minimal deformability in its interaction with the human innate immune receptor, which includes TLR 3 and TLR 9. The overall result has proven that the designed candidate vaccine is capable of modulating cell-mediated immune responses by activating the actions of CD4 T cells, natural killer cells, and macrophages, and displayed an increased memory T cell and B cell activities, which may further be validated via in vivo and in vitro techniques.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	2076-393X 2076-393X
DOI:	10.3390/vaccines10122107