Promotion of growth and resistance against Aeromonas hydrophila in Nile tilapia juveniles supplemented with Citrus limon extract

Promotion of growth and resistance against Aeromonas hydrophila in Nile tilapia juveniles supplemented with Citrus limon extract

This study aimed to investigate the effects of dietary supplementation with Citrus limon (L.) extract (CLE) on the growth, hematological, and biochemical parameters of Nile tilapia juveniles (Oreochromis niloticus L.). The chemical composition of CLE was detected using nuclear magnetic resonance and...

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Bibliographic Details
Published in:Aquaculture Vol. 578; p. 740115
Main Authors: Oliveira e Silva, Rôger, Copatti, Carlos Eduardo, Pereira, Gilmar Amaro, Macedo, Juliano dos Santos, de Souza, Anderson Miranda, Dutra, Lívia Macedo, Almeida, Jackson Roberto Guedes da Silva, Le Reste, Guillaume, Melo, José Fernando Bibiano
Format: Journal Article
Language:English
Published: Elsevier B.V 15-01-2024
Subjects:
Bacterial infection
Erythrocytes
Feed conversion ratio
Flavonoids
Weight gain
Bacterial infection
Flavonoids
Feed conversion ratio
Weight gain
Erythrocytes
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Summary:This study aimed to investigate the effects of dietary supplementation with Citrus limon (L.) extract (CLE) on the growth, hematological, and biochemical parameters of Nile tilapia juveniles (Oreochromis niloticus L.). The chemical composition of CLE was detected using nuclear magnetic resonance and mass spectrometry. Three hundred and sixty fish (12.80 ± 0.13 g) were distributed in a completely randomized experimental design with six treatments and four replicates (15 fish per tank). Six diets with different levels of CLE in the diet – 0.0 (control), 0.2, 0.4, 0.8, 1.6, and 3.2 g kg−1 – were evaluated for 60 days, followed by 8 days of Aeromonas hydrophila challenge. The bacterial inoculum was diluted in sterile saline at 1 × 107 colony-forming units mL−1. The major constituents of CLE were phenolic compounds (mainly flavonoids) and polysaccharides. Quadratic regression showed that fish supplemented with 1.6 g CLE kg diet−1 improved final weight, weight gain, feed intake, feed conversion ratio, and perivisceral fat index. Before bacterial infection, fish supplemented with 1.6 g CLE kg diet−1 showed a higher hepatosomatic index and erythrocytes (according to linear regression) and higher plasma total protein and hemoglobin (according to quadratic regression). After bacterial infection, quadratic regression showed that 0.8–3.2 g CLE kg diet−1 reduced the plasma aspartate aminotransferase levels and mortality of juveniles. In conclusion, adding 1.6 g CLE kg diet−1 is recommended to improve Nile tilapia juveniles' growth performance, health, and survival after A. hydrophila infection. •Citrus limon extract (CLE) has phenolic compounds and polysaccharides•Nile tilapia growth is increased with a 1.6 g CLE kg diet−1•1.6 g CLE kg diet−1 improved fish physiological status•CLE decreased plasma aspartate aminotransferase activity•CLE (mainly 0.8–3.2 g kg diet−1) increased fish survival after bacterial infection
ISSN:0044-8486
1873-5622
DOI:10.1016/j.aquaculture.2023.740115