Influence of different protein supplements on the recovery and in vitro maturation of bovine oocytes

Background: Oocyte quality and maturation are influenced by protein supplementation. Objective: To evaluate the influence of fetal bovine serum (FBS) and bovine serum albumin (BSA) concentrations on the recovery and in vitro maturation (IVM) of bovine oocytes. Methods: The study was divided into Sta...

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Published in:	Revista Colombiana de Ciencias Pecuarias Vol. 33; no. 3; pp. 172 - 181
Main Authors:	Azevedo Borges, Alana, Oliveira Santos, Maria V, Fernandes Pereira, Alexsandra, Fernandes França, Pedro H, Oliveira Lira, Gabriela P
Format:	Journal Article
Language:	English
Published:	Medellín Universidad de Antioquía 01-07-2020 Universidad de Antioquia
Subjects:	albumina sérica bovina Albúmina de suero bovino albúmina sérica bovina azul cresil brilhante azul de cresil brillante Bovine serum albumin Bovinos brilliant cresyl blue calidad de los ovocitos Cattle competencia meiótica competência meiótica Cultivo de embriones Cultura embrionária Embriones Embriões Embryo culture Embryos Experiments fetal bovine serum Fetal calf serum Gametocytes in vitro embryo production In vitro maturation maduración de ovocitos Maduración in vitro Maturation Maturação in vitro maturação oocitária meiotic competence oocyte maturation oocyte quality oocyte recovery Oocytes Ovum producción de embriones in vitro produção in vitro de embriões Proteins qualidade do oócito Recovery recuperación de ovocitos recuperação do oocitária Serum albumin soro fetal bovino suero fetal bovino Suplemento de alimentos Supplements Óvulo
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Summary:	Background: Oocyte quality and maturation are influenced by protein supplementation. Objective: To evaluate the influence of fetal bovine serum (FBS) and bovine serum albumin (BSA) concentrations on the recovery and in vitro maturation (IVM) of bovine oocytes. Methods: The study was divided into Stage 1 (oocyte recovery), and Stage 2 (IVM). In the first stage, three experiments were conducted according to the recovery (R) medium used: (R1) 10 vs. 20% FBS; (R2) 5 vs. 10% BSA; and (R3) the best results from R1, R2, and the combination of FBS+BSA (5+5%). Within the second stage, the maturation medium was supplemented according to three experiments: (M1) 5 vs. 10% FBS; (M2) 0.4 vs. 0.8% BSA; and (M3) better results of M1, M2, and the combination of FBS+BSA (5+0.8%). Results: In Stage 1 (R1 and R2), the media with 10% FBS and 10% BSA showed better oocyte quality results and were defined for experiment R3. In R3, the 10% FBS and the combination of FBS+BSA (5+5%) allowed recovery of better-quality oocytes. In Stage 2 (M1 and M2), media with both levels of FBS (5 and 10%) and 0.8% BSA were defined as better according to the maturation and viability rates of cumulus cells, so they were defined for experiment M3. In M3, no difference was noted among the supplements. Conclusions: For oocyte recovery, 10% FBS and the combination of FBS+BSA (5+5%) can be used to obtain immature oocytes. For the in vitro maturation, FBS (both levels, 5 and 10%) and BSA (0.8%) can be used alone or in combination.
ISSN:	0120-0690 2256-2958
DOI:	10.17533/udea.rccp.v33n3a01