Biological activities and phytochemical profile of Passiflora mucronata from the Brazilian restinga

[Display omitted] In general, Passiflora species have been reported for their folk medicinal use as sedative and anti-inflammatory. However, P. caerulea has already been reported to treat pulmonary diseases. Severe pulmonary tuberculosis, generally caused by Mycobacterium tuberculosis strains resist...

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Bibliographic Details
Published in:	Revista brasileira de farmacognosia Vol. 27; no. 6; pp. 702 - 710
Main Authors:	Araujo, Marlon H. de, Silva, Isabel C.V. da, Oliveira, Pollyana F. de, Barreto, Arielly R.R., Konno, Tatiana U.P., Esteves, Francisco de A., Barth, Thiago, Aguiar, Fernando A., Lopes, Norberto P., Dermenjian, Renee K., Guimarães, Denise O., Leal, Ivana C.R., Lasunskaia, Elena B., Muzitano, Michelle Frazão
Format:	Journal Article
Language:	English
Published:	Cham Elsevier Editora Ltda 01-11-2017 Springer International Publishing Sociedade Brasileira de Farmacognosia
Subjects:	Antimycobacterial Antioxidant Flavonoid Immunomodulatory Original Article Passifloraceae PHARMACOLOGY & PHARMACY Pharmacy Terpenes Immunomodulatory Passifloraceae Terpenes Antioxidant Flavonoid Antimycobacterial
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Summary:	[Display omitted] In general, Passiflora species have been reported for their folk medicinal use as sedative and anti-inflammatory. However, P. caerulea has already been reported to treat pulmonary diseases. Severe pulmonary tuberculosis, generally caused by Mycobacterium tuberculosis strains resistant to multiple drugs, can lead to deleterious inflammation and high mortality, encouraging new approaches in drug discovery. Thus, the aim of this work was to evaluate the Passiflora mucronata Lam., Passifloraceae, potential for tuberculosis treatment. Specifically, related to antimycobacterial activity and anti-inflammatory related effects (based on inhibition of nitric oxide, tumor necrosis factor-alpha production and antioxidant potential), as well as the chemical profile of P. mucronata. High performance liquid chromatography coupled with diode-array ultraviolet and mass spectrometer analyses of crude hydroalcoholic extract and ethyl acetate fraction showed the presence of flavonoids. Ethyl acetate fraction showed to be as antioxidant as Ginkgo biloba standard extract with EC50 of 14.61±1.25μg/ml. One major flavonoid isolated from ethyl acetate fraction was characterized as isoorientin. The hexane fraction and its main isolated compound, the triterpene β-amyrin, exhibited significant growth inhibitory activity against Mycobacterium bovis BCG (MIC50 1.61±1.43 and 3.93±1.05μg/ml, respectively). In addition, Passiflora mucronata samples, specially hexane and dichloromethane fractions, as well as pure β-amyrin, showed a dose-related inhibition of lipopolysaccharide (LPS)-induced nitric oxide production. In conclusion, Passiflora mucronata presented relevant biological potential and should be considered for further studies using in vivo pulmonary tuberculosis model.
ISSN:	0102-695X 1981-528X 1981-528X
DOI:	10.1016/j.bjp.2017.07.005