Prorocentrum lima from the South Atlantic: Morphological, molecular and toxicological characterization

Prorocentrum lima from the South Atlantic: Morphological, molecular and toxicological characterization

Morphological descriptions using light and scanning electron microscopy and molecular characterization of two Prorocentrum lima strains (UNR-01 and UNR-09) isolated from Armação dos Búzios, Rio de Janeiro, Brazil are provided. Okadaic acid (OA), dinophysistoxin-1 (DTX1) and DTX2 production by strain...

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Published in:Harmful algae Vol. 57; no. Pt A; pp. 39 - 48
Main Authors: Nascimento, Silvia M., Salgueiro, Fabiano, Menezes, Mariângela, Oliveira, Fernanda de Andréa, Magalhães, Vinicius Chiapetta Portella, De Paula, Joel Campos, Morris, Steven
Format: Journal Article
Language:English
Published: Netherlands Elsevier B.V 01-07-2016
Subjects:
algae
deformation
Dinophysistoxin
Epibenthic dinoflagellate
heat treatment
internal transcribed spacers
liquid chromatography
Liquid chromatography mass spectrometry
mass spectrometry
Molecular phylogeny
Okadaic acid
phylogeny
Prorocentrum
Prorocentrum lima
ribosomal RNA
scanning electron microscopy
toxins
Brazil
Caribbean Sea
United States
ASW, Brazil, Rio de Janeiro
USA
ASW, Caribbean Sea
AS, South Atlantic
Epibenthic dinoflagellate
Dinophysistoxin
Okadaic acid
Molecular phylogeny
Liquid chromatography mass spectrometry
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Summary:Morphological descriptions using light and scanning electron microscopy and molecular characterization of two Prorocentrum lima strains (UNR-01 and UNR-09) isolated from Armação dos Búzios, Rio de Janeiro, Brazil are provided. Okadaic acid (OA), dinophysistoxin-1 (DTX1) and DTX2 production by strain UNR-01 was investigated using liquid chromatography with mass spectrometry. Toxins were extracted from heat-treated (boiled) and non-boiled cell pellets to obtain respective quantities of free and total OA and DTX1. Growth parameters (growth rate and mean generation time) were determined for strain UNR-01. Prorocentrum lima cells were oblong-to-ovate in shape, broad in the middle region, and narrow at the anterior end. The periflagellar area was triangular, set into a V-shaped depression and was composed of eight periflagellar platelets of different sizes. The morphology fits well the characterization of the species isolated from elsewhere. Phylogenetic analysis based on internal transcribed spacer – ITS – and D1–D3 large subunit – LSU – of ribosomal RNA gene sequences revealed that both strains were identical and closely related to P. lima isolates from the Caribbean Sea and USA. The growth rate of strain UNR-01 was 0.24divday−1. OA concentrations were on average 15.2 and 38.5pg[OA]cell−1 for heat-treated and non-treated cells respectively, while DTX1 mean concentration was 0.5pg[DTX1]cell−1 for both heat-treated and non-treated cells. DTX2 was not detected. To date, these are the first strains of P. lima from the south Atlantic that have been characterized.
Bibliography:http://dx.doi.org/10.1016/j.hal.2016.05.006
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1568-9883
1878-1470
DOI:10.1016/j.hal.2016.05.006