High-fat diet causes endothelial dysfunction in the mouse ophthalmic artery

Obesity is a significant health concern that leads to impaired vascular function and subsequent abnormalities in various organs. The impact of obesity on ocular blood vessels, however, remains largely unclear. In this study, we examined the hypothesis that obesity induced by high-fat diet produces v...

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Published in:	Experimental eye research Vol. 238; p. 109727
Main Authors:	Jiang, Subao, Xia, Ning, Buonfiglio, Francesco, Böhm, Elsa W., Tang, Qi, Pfeiffer, Norbert, Olinger, Dominik, Li, Huige, Gericke, Adrian
Format:	Journal Article
Language:	English
Published:	England Elsevier Ltd 01-01-2024
Subjects:	Animals Diet, High-Fat - adverse effects Endothelial dysfunction Endothelium, Vascular - metabolism High-fat diet (HFD) Lectin-like oxidized low-density-lipoprotein receptor-1 (LOX-1) Mice Obesity Ophthalmic Artery Ophthalmic artery (OA) Oxidative Stress - physiology Reactive Oxygen Species - metabolism Receptor for advanced glycation end products (RAGE) Scavenger Receptors, Class E - genetics Scavenger Receptors, Class E - metabolism Vascular Diseases - metabolism Vasodilation Endothelial dysfunction Ophthalmic artery (OA) Lectin-like oxidized low-density-lipoprotein receptor-1 (LOX-1) High-fat diet (HFD) Receptor for advanced glycation end products (RAGE)
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Summary:	Obesity is a significant health concern that leads to impaired vascular function and subsequent abnormalities in various organs. The impact of obesity on ocular blood vessels, however, remains largely unclear. In this study, we examined the hypothesis that obesity induced by high-fat diet produces vascular endothelial dysfunction in the ophthalmic artery. Mice were subjected to a high-fat diet for 20 weeks, while age-matched controls were maintained on a standard diet. Reactivity of isolated ophthalmic artery segments was assessed in vitro. Reactive oxygen species (ROS) were quantified in cryosections by dihydroethidium (DHE) staining. Redox gene expression was determined in ophthalmic artery explants by real-time PCR. Furthermore, the expression of nicotinamide adenine dinucleotide phosphate oxidase 2 (NOX2), the receptor for advanced glycation end products (RAGE), and of the lectin-like oxidized low-density-lipoprotein receptor-1 (LOX-1) was determined in cryosections using immunofluorescence microscopy. Ophthalmic artery segments from mice on a high-fat diet exhibited impaired vasodilation responses to the endothelium-dependent vasodilator acetylcholine, while endothelium-independent responses to nitroprusside remained preserved. DHE staining intensity in the vascular wall was notably stronger in mice on a high-fat diet. Messenger RNA expression for NOX2 was elevated in the ophthalmic artery of mice subjected to high fat diet. Likewise, immunostainings revealed increased expression of NOX2 and of RAGE, but not of LOX-1. These findings suggest that a high-fat diet triggers endothelial dysfunction by inducing oxidative stress in the ophthalmic artery via involvement of RAGE and NOX2. •High-fat diet triggers endothelial dysfunction in the ophthalmic artery.•ROS generation is increased in ophthalmic arteries from mice exposed to high-fat diet.•RAGE and NOX2 expression are elevated in ophthalmic arteries from mice subjected to high-fat diet.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0014-4835 1096-0007
DOI:	10.1016/j.exer.2023.109727