Enhanced NLRP3 and DEFA1B Expression During the Active Stage of Parenchymal Neuro-Behçet's Disease

Neurological symptoms (neuro-Behçet's disease; NBD) occur in a fraction of Behçet's disease (BD) patients and often present with parenchymal brain lesions and clinical exacerbations. Our aim was to identify genes associated with attack and remission periods of NBD. Microarray analysis was...

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Bibliographic Details
Published in:	In vivo (Athens) Vol. 33; no. 5; pp. 1493 - 1497
Main Authors:	Ugurel, Elif, Erdag, Ece, Kucukali, Cem Ismail, Olcay, Ayca, Sanli, Elif, Akbayir, Ece, Kurtuncu, Murat, Gunduz, Tuncay, Yilmaz, Vuslat, Tuzun, Erdem, Vural, Burcak
Format:	Journal Article
Language:	English
Published:	Greece International Institute of Anticancer Research 01-09-2019
Subjects:	alpha-Defensins - genetics alpha-Defensins - metabolism Behcet Syndrome - diagnosis Behcet Syndrome - etiology Biomarkers Disease Progression Female Gene Expression Humans Leukocytes, Mononuclear - metabolism Magnetic Resonance Imaging Male NLR Family, Pyrin Domain-Containing 3 Protein - genetics NLR Family, Pyrin Domain-Containing 3 Protein - metabolism Phenotype Severity of Illness Index Whole Genome Sequencing Behçet's disease microarray innate immunity inflammation neuro-Behçet's disease
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Summary:	Neurological symptoms (neuro-Behçet's disease; NBD) occur in a fraction of Behçet's disease (BD) patients and often present with parenchymal brain lesions and clinical exacerbations. Our aim was to identify genes associated with attack and remission periods of NBD. Microarray analysis was performed using peripheral blood mononuclear cell (PBMC) samples obtained during attack and remission periods of five NBD patients. Expression levels of the most significantly up-regulated genes were measured with real-time PCR using PBMC samples of 15 NBD patients and 20 healthy controls. During NBD attacks, the most remarkably up-regulated genes were defensin alpha 1B (DEFA1B) and NLR family, pyrin domain containing 3 (NLRP3). Real time PCR studies showed significantly increased DEFA1B and NLRP3 expression levels during attacks. Immunological factors showing the most significant increase in expression during NBD attacks were primarily associated with innate immunity functions. DEFA1B and NLRP3 can be used as biomarkers for estimation of disease activity in NBD.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0258-851X 1791-7549
DOI:	10.21873/invivo.11629