Search Results - "Olah, Timothy"

Protein Biomarker Quantification by Immunoaffinity Liquid Chromatography-Tandem Mass Spectrometry: Current State and Future Vision by Neubert, Hendrik, Shuford, Christopher M, Olah, Timothy V, Garofolo, Fabio, Schultz, Gary A, Jones, Barry R, Amaravadi, Lakshmi, Laterza, Omar F, Xu, Keyang, Ackermann, Bradley L

“…Immunoaffinity-mass spectrometry (IA-MS) is an emerging analytical genre with several advantages for profiling and determination of protein biomarkers. Because…”
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Ultrahigh-Throughput and Chromatography-Free Bioanalysis of Polar Analytes with Acoustic Ejection Mass Spectrometry by Wagner, Andrew, Zhang, Jun, Liu, Chang, Covey, Thomas R, Olah, Timothy V, Weller, Harold (Bud) N, Shou, Wilson Z

“…Bioanalysis of polar analytes using liquid chromatography–tandem mass spectrometry (LC-MS/MS) remains a significant challenge because of their poor…”
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LC/MS/MS-based quantitation of pig and human S100A1 protein in cardiac tissues: Application to gene therapy by Sleczka, Bogdan G., Levesque, Paul C., Adam, Leonard P., Olah, Timothy V., Shipkova, Petia

“…The S100A1 protein is a target of interest for the treatment of heart failure as it has been previously reported to be depleted in failing cardiomyocytes. A…”
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Immunoaffinity enrichment LC–MS/MS quantitation of CDH17 in tissues by Caporuscio, Christian, Holmes, Derek, Olah, Timothy V, Shipkova, Petia

“…There is little information in the literature regarding assays for measuring CDH17 in tissues. Numerous studies indicate overexpression of CDH17 in a variety…”
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Quantification of Linrodostat and its metabolites: Overcoming bioanalytical challenges in support of a discovery Indoleamine 2,3 dioxygenase program by Discenza, Lorell N., Balog, Aaron, Huang, Christine, Moore, Robin, Ranasinghe, Asoka, Lin, Tai-An, D'Arienzo, Celia, Olah, Timothy V.

“…•Overcoming Bioanalytical Challenges in Support of a Discovery IDO Program.•BMS-986205 (Linrodostat) is a small molecule inhibitor of Indoleamine 2, 3…”
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A peptide immunoaffinity LC–MS/MS strategy for quantifying the GPCR protein, S1PR1 in human colon biopsies by Zhang, Hongwei, Ciccimaro, Eugene, Zalaznick, Jacob, Sleczka, Bogdan G, Menard, Laurence, Olah, Timothy V, Shipkova, Petia

“…S1PR1, a G protein-coupled receptor (GPCR) protein, is a therapeutic target for treatment of autoimmune diseases. As a potential biomarker for drug effect and…”
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An integrated Qual/Quan strategy for ganglioside lipidomics using high‐resolution mass spectrometry and Skyline software by Ranasinghe, Asoka, Ciccimaro, Eugene, D’Arienzo, Celia, Olah, Timothy V., Ponath, Paul, Hnatyshyn, Serhiy

“…Rationale Gangliosides (GS) are attractive targets in biomarker discovery because of their physiological significance in numerous human diseases including…”
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A strategy for liquid chromatography/tandem mass spectrometry based quantitation of pegylated protein drugs in plasma using plasma protein precipitation with water-miscible organic solvents and subsequent trypsin digestion to generate surrogate peptides for detection by Wu, Steven T., Ouyang, Zheng, Olah, Timothy V., Jemal, Mohammed

“…Recently, we have developed liquid chromatography/tandem mass spectrometry (LC/MS/MS)‐based methods for the quantitation of pegylated therapeutic proteins in…”
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Quantification of surrogate monoclonal antibodies in mouse serum using LC–MS/MS by Mehl, John T, Landry, France, Discenza, Lorell, Sleczka, Bogdan, Zhao, Qihong, Shuster, David J, Madia, Priyanka, DasGupta, Ruchira, Rajendran, Surendran, Sun, Huadong, Ciccimaro, Eugene, Olah, Timothy V

“…Surrogate monoclonal antibodies (mAbs) used in preclinical studies can be challenging to quantify due to lack of suitable immunoaffinity reagents or…”
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Analytical considerations for quantitative LC-MS strategies for measuring antibody drug-target engagement in tissues by Shipkova, Petia, Ciccimaro, Eugene, Olah, Timothy V

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Immunoaffinity LC-MS/MS for quantitative determination of a free and total protein target as a target engagement biomarker by Zhang, Hongwei, Gu, Huidong, Shipkova, Petia, Ciccimaro, Eugene, Sun, Huadong, Zhao, Qihong, Olah, Timothy V

“…IP-10 is a protein target for the treatment of Crohn's disease. Inhibition of IP-10 by anti-IP-10 mAbs neutralizes its various biological activities. The…”
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A universal surrogate peptide to enable LC-MS/MS bioanalysis of a diversity of human monoclonal antibody and human Fc-fusion protein drug candidates in pre-clinical animal studies by Furlong, Michael T., Ouyang, Zheng, Wu, Steven, Tamura, James, Olah, Timothy, Tymiak, Adrienne, Jemal, Mohammed

“…ABSTRACT For the development of human antibody Fc (fraction crystallizable) region‐containing therapeutic protein candidates, which can be either monoclonal…”
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2020 White Paper on Recent Issues in Bioanalysis: BMV of Hybrid Assays, Acoustic MS, HRMS, Data Integrity, Endogenous Compounds, Microsampling and Microbiome by Neubert, Hendrik, Alley, Stephen C, Lee, Anita, Jian, Wenying, Buonarati, Michael, Edmison, Anna, Garofolo, Fabio, Gorovits, Boris, Haidar, Sam, Mylott, Bill, Nouri, Parya, Qian, Mark, Vinter, Stephen, Voelker, Troy, Welink, Jan, Wu, Jiang, Yang, Eric, Yu, Hongbin, Evans, Chris, Summerfield, Scott, Wang, Jian, Bateman, Kevin, Boer, Jason, Dean, Brian, Dillen, Lieve, Faustino, Patrick, Ferrari, Luca, Hughes, Nicola, Luo, Lina, Olah, Timothy, Post, Noah, Spellman, Daniel S., Sydor, Jens, Zhang, Hui, Zhang, Jenny, Zhang, Jinhui, Fandozzi, Christine, Wilson, Amanda, Fraier, Daniela, Beaver, Christopher J., Dandamudi, Suman, Dasgupta, Arindam, Elliott, Rebecca, Ji, Allena, Li, Wenkui, McGuinness, Michael, Lima Santos, Gustavo Mendes, Mirza, Tahseen, Savoie, Natasha, Shakleya, Diaa, Sporring, Sune, Stojdl, Susan, Sundman, Phillip, Tampal, Nilufer, Woolf, Eric

“…The 14 edition of the Workshop on Recent Issues in Bioanalysis (14 WRIB) was held virtually on June 15–29, 2020 with an attendance of over 1000 representatives…”
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Mechanistic investigation of ionization suppression in electrospray ionization by King, Richard, Bonfiglio, Ryan, Fernandez-Metzler, Carmen, Miller-Stein, Cynthia, Olah, Timothy

“…We show results from experiments designed to determine the relative importance of gas phase processes and solution phase processes into ionization suppression…”
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Antibody-drug conjugate bioanalysis using LB-LC-MS/MS hybrid assays: strategies, methodology and correlation to ligand-binding assays by Wang, Jian, Gu, Huidong, Liu, Ang, Kozhich, Alexander, Rangan, Vangipuram, Myler, Heather, Luo, Linlin, Wong, Richard, Sun, Huadong, Wang, Bonnie, Vezina, Heather E, Deshpande, Shrikant, Zhang, Yan, Yang, Zheng, Olah, Timothy V, Aubry, Anne-Francoise, Arnold, Mark E, Pillutla, Renuka, DeSilva, Binodh

“…Antibody-drug conjugates (ADCs) are complex drug constructs with multiple species in the heterogeneous mixture that contribute to their efficacy and toxicity…”
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LC-MS/MS multiplexed assay for the quantitation of a therapeutic protein BMS-986089 and the target protein Myostatin by Zhu, Yongxin, D'Arienzo, Celia, Lou, Zhen, Kozhich, Alexander, Madireddi, Malavi, Chimalakonda, Anjaneya, Tymiak, Adrienne, Olah, Timothy V

“…Therapeutic protein discovery study highlights the need for the development of quantitative bioanalytical methods for determining the levels of both the…”
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The utility of stable isotope labeled (SIL) analogues in the bioanalysis of endogenous compounds by LC-MS applied to the study of bile acids in a metabolomics assay by Zheng, Joanna J., Shields, Eric E., Snow, Kimberly J., Nelson, David M., Olah, Timothy V., Reily, Michael D., Robertson, Donald G., Shipkova, Petia A., Stryker, Steven A., Xin, Baomin, Drexler, Dieter M.

“…The growing field of biomarker bioanalysis by liquid chromatography mass spectrometry (LC–MS) is challenged with the selection of suitable matrices to…”
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Increasing high-throughput Discovery bioanalysis using automated selected reaction monitoring compound optimization, ultra-high-pressure liquid chromatography, and single-step sample preparation workflows by Smalley, James, Xin, Baomin, Olah, Timothy V.

“…QuickQuan is an integrated software package for Thermo Scientific triple quadrupole mass spectrometers that allows users to automate routine operations ranging…”
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Bioanalytical strategies for developing highly sensitive liquid chromatography/tandem mass spectrometry based methods for the peptide GLP-1 agonists in support of discovery PK/PD studies by Zhang, Hongwei, Xin, Baomin, Caporuscio, Christian, Olah, Timothy V.

“…Highly sensitive liquid chromatography/tandem mass spectrometry (LC/MS/MS)‐based methods have been developed and implemented for the quantitative determination…”
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The integration of ligand binding and LC-MS-based assays into bioanalytical strategies for protein analysis by Zhang, Yan J, Olah, Timothy V, Zeng, Jianing

“…Both LBAs and LC-MS-based assays are reviewed and summarized for applications in quantitative protein analysis. A strategy for platform selection is proposed…”
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