Search Results - "Okkelman, Irina A."

A deeper understanding of intestinal organoid metabolism revealed by combining fluorescence lifetime imaging microscopy (FLIM) and extracellular flux analyses by Okkelman, Irina A., Neto, Nuno, Papkovsky, Dmitri B., Monaghan, Michael G., Dmitriev, Ruslan I.

“…Stem cells and the niche in which they reside feature a complex microenvironment with tightly regulated homeostasis, cell-cell interactions and dynamic…”
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A novel effect of DMOG on cell metabolism: direct inhibition of mitochondrial function precedes HIF target gene expression by Zhdanov, Alexander V., Okkelman, Irina A., Collins, Fergus W.J., Melgar, Silvia, Papkovsky, Dmitri B.

“…Abnormal accumulation of oncometabolite fumarate and succinate is associated with inhibition of mitochondrial function and carcinogenesis. By competing with…”
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Use of Fluorescence Lifetime Imaging Microscopy (FLIM) as a Timer of Cell Cycle S Phase by Okkelman, Irina A, Dmitriev, Ruslan I, Foley, Tara, Papkovsky, Dmitri B

“…Incorporation of thymidine analogues in replicating DNA, coupled with antibody and fluorophore staining, allows analysis of cell proliferation, but is…”
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Quantitative analysis of mucosal oxygenation using ex vivo imaging of healthy and inflamed mammalian colon tissue by Zhdanov, Alexander V., Okkelman, Irina A., Golubeva, Anna V., Doerr, Barbara, Hyland, Niall P., Melgar, Silvia, Shanahan, Fergus, Cryan, John F., Papkovsky, Dmitri B.

“…Colonic inflammation is associated with decreased tissue oxygenation, significantly affecting gut homeostasis. However, the crosstalk between O 2 consumption…”
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Properties of a cryptic lysyl oxidase from haloarchaeon Haloterrigena turkmenica by Pestov, Nikolay B, Kalinovsky, Daniel V, Larionova, Tatyana D, Zakirova, Alia Z, Modyanov, Nikolai N, Okkelman, Irina A, Korneenko, Tatyana V

“…Lysyl oxidases (LOX) have been extensively studied in mammals, whereas properties and functions of recently found homologues in prokaryotic genomes remain…”
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Live cell imaging of mouse intestinal organoids reveals heterogeneity in their oxygenation by Okkelman, Irina A., Foley, Tara, Papkovsky, Dmitri B., Dmitriev, Ruslan I.

“…Intestinal organoids are widely applied in stem cell research, regenerative medicine, toxicology, pharmacology, and host-microbe interactions research. The…”
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Balance between the cell viability and death in 3D by Debruyne, Angela C., Okkelman, Irina A., Dmitriev, Ruslan I.

“…Cell death is a phenomenon, frequently perceived as an absolute event for cell, tissue and the organ. However, the rising popularity and complexity of such 3D…”
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Cellulose-based scaffolds for fluorescence lifetime imaging-assisted tissue engineering by O'Donnell, Neil, Okkelman, Irina A., Timashev, Peter, Gromovykh, Tatyana I., Papkovsky, Dmitri B., Dmitriev, Ruslan I.

“…[Display omitted] Quantitative measurement of pH and metabolite gradients by microscopy is one of the challenges in the production of scaffold-grown organoids…”
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Live Microscopy of Multicellular Spheroids with the Multimodal Near-Infrared Nanoparticles Reveals Differences in Oxygenation Gradients by Debruyne, Angela C., Okkelman, Irina A., Heymans, Nina, Pinheiro, Cláudio, Hendrix, An, Nobis, Max, Borisov, Sergey M., Dmitriev, Ruslan I.

“…Assessment of hypoxia, nutrients, metabolite gradients, and other hallmarks of the tumor microenvironment within 3D multicellular spheroid and organoid models…”
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Estimation of the Mitochondrial Membrane Potential Using Fluorescence Lifetime Imaging Microscopy by Okkelman, Irina A., Papkovsky, Dmitri B., Dmitriev, Ruslan I.

“…Monitoring of cell metabolism represents an important application area for fluorescence lifetime imaging microscopy (FLIM). In particular, assessment of…”
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Visualization of Stem Cell Niche by Fluorescence Lifetime Imaging Microscopy by Okkelman, Irina A, Puschhof, Jens, Papkovsky, Dmitri B, Dmitriev, Ruslan I

“…Fluorescence lifetime imaging microscopy (FLIM), enabling live quantitative multiparametric analyses, is an emerging bioimaging approach in tissue engineering…”
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Nanoparticle‐Based Fluoroionophore for Analysis of Potassium Ion Dynamics in 3D Tissue Models and In Vivo by Müller, Bernhard J., Zhdanov, Alexander V., Borisov, Sergey M., Foley, Tara, Okkelman, Irina A., Tsytsarev, Vassiliy, Tang, Qinggong, Erzurumlu, Reha S., Chen, Yu, Zhang, Haijiang, Toncelli, Claudio, Klimant, Ingo, Papkovsky, Dmitri B., Dmitriev, Ruslan I.

“…The imaging of real‐time fluxes of K+ ions in live cell with high dynamic range (5–150 × 10−3m) is of paramount importance for neuroscience and physiology of…”
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Production and Multi-Parameter Live Cell Fluorescence Lifetime Imaging Microscopy (FLIM) of Multicellular Spheroids by Debruyne, Angela C, Ferrari, Gabriele, Zhou, Hang, Van Loon, Nore, Heymans, Nina, Okkelman, Irina A, Dmitriev, Ruslan I

“…Multicellular tumor spheroids are a popular 3D tissue microaggregate model for reproducing tumor microenvironment, testing and optimizing drug therapies and…”
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Multi-Parametric Imaging of Hypoxia and Cell Cycle in Intestinal Organoid Culture by Okkelman, Irina A, Foley, Tara, Papkovsky, Dmitri B, Dmitriev, Ruslan I

“…Dynamics of oxygenation of tissue and stem cell niches are important for understanding physiological function of the intestine in normal and diseased states…”
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Affordable Oxygen Microscopy-Assisted Biofabrication of Multicellular Spheroids by Okkelman, Irina A, Vercruysse, Chris, Kondrashina, Alina V, Borisov, Sergey M, Dmitriev, Ruslan I

“…Multicellular spheroids are important tools for studying tissue and cancer physiology in 3D and are frequently used in tissue engineering as tissue assembling…”
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Phosphorescent Oxygen and Mechanosensitive Nanostructured Materials Based on Hard Elastic Polypropylene Films by Okkelman, Irina A, Dolgova, Alla A, Banerjee, Swagata, Kerry, Joseph P, Volynskii, Aleksandr, Arzhakova, Olga V, Papkovsky, Dmitri B

“…It is well known that sensitivity of quenched-phosphorescence O2 sensors can be tuned by changing the nature of indicator dye and host polymer acting as…”
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Extracellular Ca2+-Sensing Fluorescent Protein Biosensor Based on a Collagen-Binding Domain by Okkelman, Irina A, McGarrigle, Ryan, O’Carroll, Shane, Berrio, Daniel Carvajal, Schenke-Layland, Katja, Hynes, James, Dmitriev, Ruslan I

“…The importance of extracellular gradients of biomolecules is increasingly appreciated in the processes of tissue development and regeneration, in health and…”
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Evolutionary diversification of the BetaM interactome acquired through co-option of the ATP1B4 gene in placental mammals by Korneenko, Tatyana V., Pestov, Nikolay B., Ahmad, Nisar, Okkelman, Irina A., Dmitriev, Ruslan I., Shakhparonov, Mikhail I., Modyanov, Nikolai N.

“…ATP1B4 genes represent a rare instance of orthologous vertebrate gene co-option that radically changed properties of the encoded BetaM proteins, which function…”
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Nuclear translocation of lysyl oxidase is promoted by interaction with transcription repressor p66β by Okkelman, Irina A, Sukaeva, Alia Z, Kirukhina, Ekaterina V, Korneenko, Tatyana V, Pestov, Nikolay B

“…Lysyl oxidase (LOX) is an amine oxidase involved in protein cross-linking of the extracellular matrix. Less well characterized is the role that LOX plays among…”
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Two Distinct Nuclear Localization Signals in Mammalian MSL1 Regulate Its Function by Dmitriev, Ruslan I., Pestov, Nikolay B., Shakhparonov, Mikhail I., Okkelman, Irina A.

“…ABSTRACT MSL1 protein regulates global histone H4 acetylation at residue K16 in stem and cancer cells, through interaction with KAT8. The functional…”
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