Viability of erythrocytes in canine packed red blood cells stored in CPDA-1 is related to the presence of Mycoplasma haemocanis

Hemotropic mycoplasmas are associated with subclinical disease in dogs and should be identified in blood donors. The objective was to investigate the presence and effect of M. haemocanis in units of packed red blood cells (pRBC) during storage. Canine donors (n = 10) were screened for M. haemocanis...

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Published in:	Comparative immunology, microbiology and infectious diseases Vol. 97; p. 101982
Main Authors:	Camargo Castillo, Monica A., de Almeida, Bruno Albuquerque, Wissmann, Daiani, Moreira, Renata Fagundes, Okano, Felipe Yuki, Gonzalez, Felix H.D., Soares, João Fábio, de Faria Valle, Stella
Format:	Journal Article
Language:	English
Published:	England Elsevier Ltd 01-06-2023
Subjects:	Animals Blood products Canine hemoplasmas Dog Diseases Dogs Erythrocytes Glucose - pharmacology Mycoplasma Mycoplasma Infections - veterinary Storage lesion Transfusion medicine Storage lesion Transfusion medicine Blood products Canine hemoplasmas
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Summary:	Hemotropic mycoplasmas are associated with subclinical disease in dogs and should be identified in blood donors. The objective was to investigate the presence and effect of M. haemocanis in units of packed red blood cells (pRBC) during storage. Canine donors (n = 10) were screened for M. haemocanis by quantitative real-time PCR. pRBCs were obtained from 5 hemoplasma negative dogs and 5 hemoplasma positive dogs. Each pRBC was aliquoted into two 100 mL transfer bags and stored at 4 °C. M. haemocanis loads and biochemical variables (pH, bicarbonate, potassium, sodium, chlorite, glucose, lactate, ammonia, PCV, and % hemolysis) were evaluated on days 1, 7, 18, and 29. M. haemocanis loads increased in pRBC from day 1–29 of storage. Glucose decreased and lactate increase faster in pRBC with M. haemocanis. This study contributes to understand hemoplasma metabolism and reinforces that dog donors should be tested for hemoplasmas. •Mycoplasma haemocanis DNA increased in pRBCS after 29 days of storage.•Changes in glucose and lactate of stored pRBC occur faster in M. haemocanis presence.•Blood donor should be screened for M. haemocanis, as it could remain viable in during pRBC storage.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0147-9571 1878-1667
DOI:	10.1016/j.cimid.2023.101982