New cytokine dressings. II. Stimulation of oxidative burst in leucocytes in vitro and reduction of viable bacteria within an infected wound

Recently, we have developed the new dressings containing rhG-CSF or rhGM-CSF. In the present study we investigated either in vitro or in vivo biological activity of the dressings. Human whole blood samples were incubated with extracts from the collagen- or polyurethane-based dressings containing rhG...

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Published in:	International journal of pharmaceutics Vol. 184; no. 2; pp. 179 - 187
Main Authors:	Grzybowski, Jacek, Janiak, Marek K, Oidak, Ewa, Lasocki, Krzysztof, Wrembel-Wargocka, Jolanta, Cheda, Aneta, Antos-Bielska, Malgorzata, Pojda, Zygmunt
Format:	Journal Article
Language:	English
Published:	Amsterdam Elsevier B.V 20-07-1999 Elsevier
Subjects:	Animals Antibacterial agents Antibiotics. Antiinfectious agents. Antiparasitic agents Bacteria - drug effects Bandages Biological and medical sciences Cytokine dressings Cytokines - administration & dosage Cytokines - pharmacology Drug Delivery Systems Epidermal Growth Factor - administration & dosage Epidermal Growth Factor - pharmacology Flow Cytometry General pharmacology Granulocyte Colony-Stimulating Factor - administration & dosage Granulocyte Colony-Stimulating Factor - pharmacology Granulocyte-Macrophage Colony-Stimulating Factor - administration & dosage Granulocyte-Macrophage Colony-Stimulating Factor - pharmacology Humans Leukocyte Count Leukocytes - drug effects Leukocytes - metabolism Medical sciences Mice Mice, Inbred C3H Murine model of infected wound Oxidative burst Phagocytic activity Phagocytosis - drug effects Pharmaceutical technology. Pharmaceutical industry Pharmacology. Drug treatments Recombinant Proteins - administration & dosage Recombinant Proteins - pharmacology Respiratory Burst - drug effects rhG-CSF rhGM-CSF Topical cytokine-delivery system Wound Healing - drug effects Wounds and Injuries - microbiology Cytokine dressings rhG-CSF Oxidative burst Phagocytic activity rhGM-CSF Topical cytokine-delivery system Murine model of infected wound Pseudomonadales Skin disease Wound Dressing Granulocyte colony stimulating factor Bacteria Pseudomonadaceae Pseudomonas aeruginosa Recombinant protein Delivery system Pharmaceutical technology Rodentia Cytokine Biological activity Infection Vertebrata Mammalia Mouse Animal Collagen Polyurethane Dosage form Antibacterial agent Granulocyte macrophage colony stimulating factor Phagocytosis Topical administration
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Summary:	Recently, we have developed the new dressings containing rhG-CSF or rhGM-CSF. In the present study we investigated either in vitro or in vivo biological activity of the dressings. Human whole blood samples were incubated with extracts from the collagen- or polyurethane-based dressings containing rhG-CSF or/and rhGM-CSF and phagocytic and oxidative metabolic activities were quantitated using Phagotest or Bursttest kits. The results indicate that both the number of phagocyting cells and the intensity of phagocytosis per cell, as well as the level of the oxidative burst in particular, were stimulated by one or both of the cytokines extracted. Next, the experimental skin wounds in mice were infected with 10 7 CFU of Pseudomonas aeruginosa strain ATCC 27853 and treated locally with the rhG-CSF-containing dressing. The analysis of the biopsies taken from the wounds indicated that in mice treated with the cytokine-containing dressing on the third day the log of CFU per biopsy was 5.0 vs. 6.2 in the control ( P<0.001), and on the 8th day was lower than 4 vs. 5.4 in control ( P<0.0001). Our findings clearly suggest that the newly designed dressings containing the incorporated CSFs can be used as effective topical cytokine-delivery system in the treatment of bacterial infections in wounds.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0378-5173 1873-3476
DOI:	10.1016/S0378-5173(99)00064-2