Search Results - "Ohtani, Naoto"

An Extreme Thermophile, Thermus thermophilus, Is a Polyploid Bacterium by OHTANI, Naoto, TOMITA, Masaru, ITAYA, Mitsuhiro

“…Article Usage Stats Services JB Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley…”
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The third plasmid pVV8 from Thermus thermophilus HB8: isolation, characterization, and sequence determination by Ohtani, Naoto, Tomita, Masaru, Itaya, Mitsuhiro

“…The extremely thermophilic bacterium Thermus thermophilus is a model organism for structural biology and systems biology, and the so-called “Structural and…”
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Identification of a novel nucleoside triphosphatase from Mycoplasma mobile: a prime candidate motor for gliding motility by Ohtani, Naoto, Miyata, Makoto

“…A protein with a molecular mass of 42 kDa (P42) from Mycoplasma mobile, one of several mycoplasmas that exhibit gliding motility, was shown to be a novel…”
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Junction ribonuclease activity specified in RNases HII/2 by Ohtani, Naoto, Tomita, Masaru, Itaya, Mitsuhiro

“…Junction ribonuclease (JRNase) recognizes the transition from RNA to DNA of an RNA–DNA/DNA hybrid, such as an Okazaki fragment, and cleaves it, leaving a…”
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Junction ribonuclease: a ribonuclease HII orthologue from Thermus thermophilus HB8 prefers the RNA-DNA junction to the RNA/DNA heteroduplex by Ohtani, Naoto, Tomita, Masaru, Itaya, Mitsuhiro

“…The genome of an extremely thermophilic bacterium, Thermus thermophilus HB8, contains a single ORF (open reading frame) encoding an RNase-HII-like sequence…”
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Restriction on conjugational transfer of pLS20 in Bacillus subtilis 168 by Ohtani, N.(Keio Univ., Tsuruoka, Yamagata (Japan). Inst. for Advanced Biosciences), Sato, M, Tomita, M, Itaya, M

“…Conjugational transfer of pLS20 in Bacillus subtilis Marburg 168 is restricted by the BsuM restriction-modification system. Restriction efficiency was measured…”
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Identification of the first archaeal Type 1 RNase H gene from Halobacterium sp. NRC-1: archaeal RNase HI can cleave an RNA-DNA junction by Ohtani, Naoto, Yanagawa, Hiroshi, Tomita, Masaru, Itaya, Mitsuhiro

“…All the archaeal genomes sequenced to date contain a single Type 2 RNase H gene. We found that the genome of a halophilic archaeon, Halobacterium sp. NRC-1,…”
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Conjugational transfer system to shuttle giant DNA cloned by Bacillus subtilis genome (BGM) vector by Kuroki, Azusa, Ohtani, Naoto, Tsuge, Kenji, Tomita, Masaru, Itaya, Mitsuhiro

“…The Bacillus subtilis GenoMe (BGM) vector was designed as a versatile vector for the cloning of giant DNA segments. Cloned DNA in the BGM can be retrieved to a…”
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Identification of RNase HII from psychrotrophic bacterium, Shewanella sp. SIB1 as a high‐activity type RNase H by Chon, Hyongi, Tadokoro, Takashi, Ohtani, Naoto, Koga, Yuichi, Takano, Kazufumi, Kanaya, Shigenori

“…The gene encoding RNase HII from the psychrotrophic bacterium, Shewanella sp. SIB1 was cloned, overexpressed in Escherichia coli, and the recombinant protein…”
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Gene cloning and biochemical characterizations of thermostable ribonuclease HIII from Bacillus stearothermophilus by Chon, H. (Osaka Univ., Suita (Japan)), Nakano, R, Ohtani, N, Haruki, M, Takano, K, Morikawa, M, Kanaya, S

“…The gene encoding RNase HIII from the thermophmcbacterium Bacillus stearothermophilus was eloned and overexpressed in Escherichia coli,and the recombinant…”
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Gene cloning, overproduction, and characterization of thermolabile alkaline phosphatase from a psychrotrophic bacterium by Suzuki, Y. (Osaka Univ., Suita (Japan)), Mizutani, Y, Tsuji, T, Ohtani, N, Takano, K, Haruki, M, Morikawa, M, Kanaya, S

“…The gene encoding alkaline phosphatase from the psychrotrophic bacterium Shewanella sp. SIB1 was cloned, sequenced, and overexpressed in Escherichia coli. The…”
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Characterization of Ribonuclease HII from Escherichia coli Overproduced in a Soluble Form by Ohtani, Naoto, Haruki, Mitsuru, Muroya, Ayumu, Morikawa, Masaaki, Kanaya, Shigenori

“…Escherichia coli RNase mi 1b composed of 198 amino acid residues. The enzyme has been overproduced in an insoluble form, purified in a urea-denatured form, and…”
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Curing the Megaplasmid pTT27 from Thermus thermophilus HB27 and Maintaining Exogenous Plasmids in the Plasmid-Free Strain by Ohtani, Naoto, Tomita, Masaru, Itaya, Mitsuhiro

“…Stepwise deletions in the only plasmid in Thermus thermophilus HB27, megaplasmid pTT27, showed that two distantly located loci were important for maintenance…”
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Molecular diversities of RNases H by Ohtani, Naoto, Haruki, Mitsuru, Morikawa, Masaaki, Kanaya, Shigenori

“…RNase H is an enzyme that specifically cleaves RNA hybridized to DNA. The enzyme is ubiquitously present in various organisms. Single bacterial and eucaryotic…”
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Importance of an N-terminal extension in ribonuclease HII from Bacillus stearothermophilus for substrate binding by Muroya, Ayumu, Nakano, Rikita, Ohtani, Naoto, Haruki, Mitsuru, Morikawa, Masaaki, Kanaya, Shigenori

“…The gene encoding ribonuclease HII from Bacillus stearothermophilus was cloned and expressed in Escherichia coli. The overproduced protein, Bst-RNase HII, was…”
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Stable and efficient delivery of DNA to Bacillus subtilis by Itaya, Mitsuhiro, Nagasaku, Mayumi, Shimada, Tomoe, Ohtani, Naoto, Shiwa, Yuh, Yoshikawa, Hirofumi, Kaneko, Shinya, Tomita, Masaru, Sato, Mitsuru

“…Bacillus subtilis (natto) is generally regarded as a safe bacterium and used as a host for the production of several materials. However, genetic engineering of…”
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Stable and efficient delivery of DNA to Bacillus subtilis (natto) using pLS20 conjugational transfer plasmids by Itaya, Mitsuhiro, Nagasaku, Mayumi, Shimada, Tomoe, Ohtani, Naoto, Shiwa, Yuh, Yoshikawa, Hirofumi, Kaneko, Shinya, Tomita, Masaru, Sato, Mitsuru

“…Bacillus subtilis (natto) is generally regarded as a safe bacterium and used as a host for the production of several materials. However, genetic engineering of…”
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Identification of a replication initiation protein of the pVV8 plasmid from Thermus thermophilus HB8 by Ohtani, Naoto, Tomita, Masaru, Itaya, Mitsuhiro

“…Recently, the extremely thermophilic bacterium Thermus thermophilus HB8 has been demonstrated to harbor a circular plasmid designated by pVV8 in addition to…”
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Serial assembly of Thermus megaplasmid DNA in the genome of Bacillus subtilis 168: A BAC-based domino method applied to DNA with a high GC content by Ohtani, Naoto, Hasegawa, Miki, Sato, Mitsuru, Tomita, Masaru, Kaneko, Shinya, Itaya, Mitsuhiro

“…Bacillus subtilis is the only bacterium‐based host able to clone giant DNA above 1000 kbp. DNA previously handled by this host was limited to that with GC…”
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Cleavage of double-stranded RNA by RNase HI from a thermoacidophilic archaeon, Sulfolobus tokodaii 7 by Ohtani, Naoto, Yanagawa, Hiroshi, Tomita, Masaru, Itaya, Mitsuhiro

“…ST0753, the orthologous gene of Type 1 RNase H found in a thermoacidophilic archaeon, Sulfolobus tokodaii, was analyzed. The recombinant ST0753 protein…”
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