Search Results - "Ohtake, Hisao"
-
1
In vitro production of n-butanol from glucose
Published in Metabolic engineering (01-11-2013)“…The heat treatment of recombinant mesophiles having heterologous thermotolerant enzymes results in the one-step preparation of highly selective biocatalytic…”
Get full text
Journal Article -
2
Assembly and multiple gene expression of thermophilic enzymes in Escherichia coli for in vitro metabolic engineering
Published in Biotechnology and bioengineering (01-01-2015)“…ABSTRACT In vitro reconstitution of an artificial metabolic pathway is an emerging approach for the biocatalytic production of industrial chemicals. However,…”
Get full text
Journal Article -
3
Direct conversion of glucose to malate by synthetic metabolic engineering
Published in Journal of biotechnology (10-03-2013)“…► An in vitro metabolic pathway for conversion of glucose to malate was constructed. ► Reversible carboxylation was coupled with a non-ATP-forming glycolysis…”
Get full text
Journal Article -
4
Use of hydrophobic bacterium Rhodococcus rhodochrous NBRC15564 expressed thermophilic alcohol dehydrogenases as whole-cell catalyst in solvent-free organic media
Published in Process biochemistry (1991) (01-05-2013)“…•The hydrophobic bacteria, Rhodococcus rhodochrous could be well dispersed in organic media.•R. rhodochrous showed the highest affinity to hydrophobic…”
Get full text
Journal Article -
5
In vitro metabolic engineering for the salvage synthesis of NAD
Published in Metabolic engineering (01-05-2016)“…Excellent thermal and operational stabilities of thermophilic enzymes can greatly increase the applicability of biocatalysis in various industrial fields…”
Get full text
Journal Article -
6
Inhibition of Quorum Sensing in Pseudomonas aeruginosa by N-Acyl Cyclopentylamides
Published in Applied and Environmental Microbiology (01-05-2007)“…N-Octanoyl cyclopentylamide (C8-CPA) was found to moderately inhibit quorum sensing in Pseudomonas aeruginosa PAO1. To obtain more powerful inhibitors, a…”
Get full text
Journal Article -
7
Construction of BAC-based physical map and analysis of chromosome rearrangement in chinese hamster ovary cell lines
Published in Biotechnology and bioengineering (01-06-2012)“…Chinese hamster ovary (CHO) cells have frequently been used in biotechnology for many years as a mammalian host cell platform for cloning and expressing genes…”
Get full text
Journal Article -
8
Synthetic metabolic engineering-a novel, simple technology for designing a chimeric metabolic pathway
Published in Microbial cell factories (06-09-2012)“…The integration of biotechnology into chemical manufacturing has been recognized as a key technology to build a sustainable society. However, the practical…”
Get full text
Journal Article -
9
The potential for livestock manure valorization and phosphorus recovery by hydrothermal technology - a critical review
Published in Materials science for energy technologies (2023)“…Carbon neutrality has become one of the major challenges to the global sustainable development. The sector of livestock & manure, which accounts for 5.8% of…”
Get full text
Journal Article -
10
A simple technology for phosphorus recovery using acid-treated concrete sludge
Published in Separation and purification technology (13-06-2016)“…[Display omitted] •Acid-treated concrete sludge (A-CS) was used for phosphate (Pi) recovery.•The Pi removal efficiency of CS particles was improved by soaking…”
Get full text
Journal Article -
11
Bio-Based production of rock phosphate for phosphorus recycling
Published in Journal of bioscience and bioengineering (01-11-2009)Get full text
Journal Article -
12
Isolation and characterization of a thermotolerant ene reductase from Geobacillus sp. 30 and its heterologous expression in Rhodococcus opacus
Published in Applied microbiology and biotechnology (01-07-2014)“…Rhodococcus opacus B-4 cells are adhesive to and even dispersible in water-immiscible hydrocarbons owing to their highly lipophilic nature. In this study, we…”
Get full text
Journal Article -
13
A mobile pilot-scale plant for in situ demonstration of phosphorus recovery from wastewater using amorphous calcium silicate hydrates
Published in Separation and purification technology (01-10-2016)“…[Display omitted] •A mobile pilot-scale plant was developed for in situ phosphate (Pi) recovery.•Amorphous calcium silicate hydrates (A-CSHs) were used for Pi…”
Get full text
Journal Article -
14
Redirection of the Reaction Specificity of a Thermophilic Acetolactate Synthase toward Acetaldehyde Formation
Published in PloS one (05-01-2016)“…Acetolactate synthase and pyruvate decarboxylase are thiamine pyrophosphate-dependent enzymes that convert pyruvate into acetolactate and acetaldehyde,…”
Get full text
Journal Article -
15
Development of a Continuous Bioconversion System Using a Thermophilic Whole-Cell Biocatalyst
Published in Applied and Environmental Microbiology (01-03-2013)“…Classifications Services AEM Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley Reddit…”
Get full text
Journal Article -
16
Phenotyping the quality of complex medium components by simple online-monitored shake flask experiments
Published in Microbial cell factories (07-11-2014)“…Media containing yeast extracts and other complex raw materials are widely used for the cultivation of microorganisms. However, variations in the specific…”
Get full text
Journal Article -
17
Online measurement of the respiratory activity in shake flasks enables the identification of cultivation phases and patterns indicating recombinant protein production in various Escherichia coli host strains
Published in Biotechnology progress (01-03-2018)“…Escherichia coli is commonly used for recombinant protein production with many available host strains. Screening experiments are often performed in batch mode…”
Get full text
Journal Article -
18
Rapid construction of transgene-amplified CHO cell lines by cell cycle checkpoint engineering
Published in Applied microbiology and biotechnology (01-07-2013)“…The process of establishing high-producing cell lines for the manufacture of therapeutic proteins is usually both time-consuming and laborious due to the low…”
Get full text
Journal Article -
19
Improved production of recombinant human antithrombin III in Chinese hamster ovary cells by ATF4 overexpression
Published in Biotechnology and bioengineering (01-06-2008)“…To improve the production of recombinant human antithrombin III (AT‐III) in Chinese hamster ovary (CHO) cells, the genes encoding transcription factors, ATF4…”
Get full text
Journal Article -
20
Glycosylation analysis of an aggregated antibody produced by Chinese hamster ovary cells in bioreactor culture
Published in Journal of bioscience and bioengineering (01-05-2014)“…N-Glycosylation of therapeutic antibodies contributes not only to their biological function, but also to their stability and tendency to aggregate. Here, we…”
Get full text
Journal Article