Search Results - "Ohrt, Thomas"

Intracellular localization and routing of miRNA and RNAi pathway components by Ohrt, Thomas, Muetze, Joerg, Svoboda, Petr, Schwille, Petra

“…Several different pathways, generally termed RNA silencing pathways, utilize small RNA molecules guiding sequence-specific silencing effects of…”
Get more information

Characterization of Protein Dynamics in Asymmetric Cell Division by Scanning Fluorescence Correlation Spectroscopy by Petrášek, Zdeněk, Hoege, Carsten, Mashaghi, Alireza, Ohrt, Thomas, Hyman, Anthony A., Schwille, Petra

“…The development and differentiation of complex organisms from the single fertilized egg is regulated by a variety of processes that all rely on the…”
Get full text

c-Cbl binds to tyrosine-phosphorylated neurotrophin receptor p75 and induces its ubiquitination by Ohrt, Thomas, Mancini, Annalisa, Tamura, Teruko, Niedenthal, Rainer

“…The p75 neurotrophin receptor (p75 NTR) has dual functions in cell survival and cell death but its intracellular signalling pathways are not understood. Here…”
Get full text

Surgical Coaching from Head-Mounted Video in the Training of Fluoroscopically Guided Articular Fracture Surgery by Karam, Matthew D., MD, Koehler, Daniel M., MD, Westerlind, Brian O, Marsh, J.Lawrence, Thomas, Geb W., PhD, Lafferty, Paul M., MD, Van Heest, Ann E., MD, Ohrt, Gary Thomas, MS, Anderson, Donald D., PhD

“…Background The evolving surgical skills education paradigm in orthopaedics has generated a strong demand for validated educational tools and methodologies…”
Get full text

Fluorescence correlation spectroscopy and fluorescence cross-correlation spectroscopy reveal the cytoplasmic origination of loaded nuclear RISC in vivo in human cells by Ohrt, Thomas, Mütze, Jörg, Staroske, Wolfgang, Weinmann, Lasse, Höck, Julia, Crell, Karin, Meister, Gunter, Schwille, Petra

“…Studies of RNA interference (RNAi) provide evidence that in addition to the well-characterized cytoplasmic mechanisms, nuclear mechanisms also exist. The…”
Get full text

Importin 8 Is a Gene Silencing Factor that Targets Argonaute Proteins to Distinct mRNAs by Weinmann, Lasse, Höck, Julia, Ivacevic, Tomi, Ohrt, Thomas, Mütze, Jörg, Schwille, Petra, Kremmer, Elisabeth, Benes, Vladimir, Urlaub, Henning, Meister, Gunter

“…Small regulatory RNAs including small interfering RNAs (siRNAs) and microRNAs (miRNAs) guide Argonaute (Ago) proteins to specific target RNAs leading to mRNA…”
Get full text

Prp2-mediated protein rearrangements at the catalytic core of the spliceosome as revealed by dcFCCS by Ohrt, Thomas, Prior, Mira, Dannenberg, Julia, Odenwälder, Peter, Dybkov, Olexandr, Rasche, Nicolas, Schmitzová, Jana, Gregor, Ingo, Fabrizio, Patrizia, Enderlein, Jörg, Lührmann, Reinhard

“…The compositional and conformational changes during catalytic activation of the spliceosome promoted by the DEAH box ATPase Prp2 are only poorly understood…”
Get full text

Molecular dissection of step 2 catalysis of yeast pre-mRNA splicing investigated in a purified system by Ohrt, Thomas, Odenwälder, Peter, Dannenberg, Julia, Prior, Mira, Warkocki, Zbigniew, Schmitzová, Jana, Karaduman, Ramazan, Gregor, Ingo, Enderlein, Jörg, Fabrizio, Patrizia, Lührmann, Reinhard

“…Step 2 catalysis of pre-mRNA splicing entails the excision of the intron and ligation of the 5' and 3' exons. The tasks of the splicing factors Prp16, Slu7,…”
Get full text

Fluorescence correlation spectroscopy in vivo by Mütze, J., Ohrt, T., Schwille, P.

“…This review will focus on the application and potential of FCS and FCCS in vivo. Practical issues and sources of artifacts when performing measurements in…”
Get full text

Fluorescence Cross-Correlation Spectroscopy Reveals Mechanistic Insights into the Effect of 2′-O-Methyl Modified siRNAs in Living Cells by Ohrt, Thomas, Staroske, Wolfgang, Mütze, Jörg, Crell, Karin, Landthaler, Markus, Schwille, Petra

“…RNA interference (RNAi) offers a powerful tool to specifically direct the degradation of complementary RNAs, and thus has great therapeutic potential for…”
Get full text

In situ fluorescence analysis demonstrates active siRNA exclusion from the nucleus by Exportin 5 by Ohrt, Thomas, Merkle, Dennis, Birkenfeld, Karin, Echeverri, Christophe J., Schwille, Petra

“…Two types of short double-stranded RNA molecules, namely microRNAs (miRNAs) and short interfering RNAs (siRNAs), have emerged recently as important regulators…”
Get full text

siRNA modifications and sub-cellular localization: a question of intracellular transport? by Ohrt, T, Schwille, P

“…RNA interference (RNAi) is an evolutionary conserved post-transcriptional gene silencing mechanism, in which double stranded RNA effector molecules trigger the…”
Get more information

Cellular Dynamics of Ku: Characterization and Purification of Ku-eGFP by Merkle, Dennis, Zheng, Dan, Ohrt, Thomas, Crell, Karin, Schwille, Petra

“…Ku is a predominantly nuclear protein that functions as a DNA double-strand-break (DSB) binding protein and regulatory subunit of the DNA-dependent protein…”
Get full text

Nuclear RISC Originates from Cytoplasmic Loaded RISC in Human Cells by Muetze, Joerg, Ohrt, Thomas, Staroske, Wolfgang, Crell, Karin, Schwille, Petra

Get full text

Fluorescence Correlation Spectroscopy Elucidates the Pathway of RNA Interference by Mütze, Jörg, Ohrt, Thomas, Staroske, Wolfgang, Weinmann, Lasse, Höck, Julia, Meister, Gunter, Schwille, Petra

Get full text

Surgical simulation training models for orthopaedic fracture surgery by Ohrt, Gary Thomas

“…Articular fracture reduction is a complex surgical task that requires surgeons to be competent at multiple surgical skills to successfully complete. The list…”
Get full text

Surgical simulation training models for orthopaedic fracture surgery by Ohrt, Gary Thomas

“…Articular fracture reduction is a complex surgical task that requires surgeons to be competent at multiple surgical skills to successfully complete. The list…”
Get full text