Overexpression of the PHO84 gene causes heavy metal accumulation and induces Ire1p-dependent unfolded protein response in Saccharomyces cerevisiae cells

Pho84p, the protein responsible for the high-affinity uptake and transport of inorganic phosphate across the plasma membrane, is also involved in the low-affinity uptake of heavy metals in the Saccharomyces cerevisiae cells. In the present study, the effect of PHO84 overexpression upon the heavy met...

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Bibliographic Details
Published in:	Applied microbiology and biotechnology Vol. 94; no. 2; pp. 425 - 435
Main Authors:	Ofiteru, Augustin Minel, Ruta, Lavinia Liliana, Rotaru, Codruta, Dumitru, Ioana, Ene, Cristian Dumitru, Neagoe, Aurora, Farcasanu, Ileana Cornelia
Format:	Journal Article
Language:	English
Published:	Berlin/Heidelberg Springer-Verlag 01-04-2012 Springer Springer Nature B.V
Subjects:	Accumulation Analysis Applied Genetics and Molecular Biotechnology Bioaccumulation Biomedical and Life Sciences Biotechnology Brewer's yeast Carrier proteins Gene Expression Genetic aspects Health aspects Heavy metals Homeostasis Identification and classification Life Sciences Membrane Glycoproteins - metabolism Metabolism Metals Metals, Heavy - metabolism Microbial Genetics and Genomics Microbiology Physiological aspects Plasma Plasmids Protein-Serine-Threonine Kinases - metabolism Proteins Proton-Phosphate Symporters - metabolism Saccharomyces cerevisiae Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae - metabolism Saccharomyces cerevisiae - physiology Saccharomyces cerevisiae Proteins - metabolism Studies Unfolded Protein Response Yeast Yeasts Overexpression Heavy metal accumulation
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Summary:	Pho84p, the protein responsible for the high-affinity uptake and transport of inorganic phosphate across the plasma membrane, is also involved in the low-affinity uptake of heavy metals in the Saccharomyces cerevisiae cells. In the present study, the effect of PHO84 overexpression upon the heavy metal accumulation by yeast cells was investigated. As PHO84 overexpression triggered the Ire1p-dependent unfolded protein response, abundant plasma membrane Pho84p could be achieved only in ire1Δ cells. Under environmental surplus, PHO84 overexpression augmented the metal accumulation by the wild type, accumulation that was exacerbated by the IRE1 deletion. The pmr1Δ cells, lacking the gene that encodes the P-type ATPase ion pump that transports Ca 2+ and Mn 2+ into the Golgi, hyperaccumulated Mn 2+ even from normal medium when overexpressing PHO84 , a phenotype which is rather restricted to metal-hyperaccumulating plants.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0175-7598 1432-0614
DOI:	10.1007/s00253-011-3784-3