Detection and characterisation of swine hepatitis E virus in New Zealand

The objectives of the present study were to establish the presence of hepatitis E virus (HEV) in New Zealand pigs, first by testing for HEV antibody in pig herds throughout New Zealand to measure the herd prevalence, then by attempting to amplify HEV genomic sequences by PCR. Antibody was measured b...

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Bibliographic Details
Published in:	Journal of medical virology Vol. 65; no. 3; pp. 525 - 529
Main Authors:	Garkavenko, Olga, Obriadina, Anna, Meng, Jihong, Anderson, David A., Benard, Helen J., Schroeder, Bryan A., Khudyakov, Yury E., Fields, Howard A., Croxson, Margaret C.
Format:	Journal Article
Language:	English
Published:	New York John Wiley & Sons, Inc 01-11-2001 Wiley-Liss
Subjects:	Animals Biological and medical sciences Feces - virology Fundamental and applied biological sciences. Psychology Hepatitis Antibodies - blood Hepatitis E - epidemiology Hepatitis E - veterinary Hepatitis E - virology Hepatitis E virus Hepatitis E virus - classification Hepatitis E virus - genetics Hepatitis E virus - immunology Hepatitis E virus - isolation & purification Humans Microbiology Molecular Sequence Data New Zealand - epidemiology phylogenetic analysis Phylogeny Reverse Transcriptase Polymerase Chain Reaction RNA, Viral - analysis Sequence Analysis, DNA Swine Swine Diseases - epidemiology Swine Diseases - virology zoonoses Zoonoses - virology New Zealand
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Summary:	The objectives of the present study were to establish the presence of hepatitis E virus (HEV) in New Zealand pigs, first by testing for HEV antibody in pig herds throughout New Zealand to measure the herd prevalence, then by attempting to amplify HEV genomic sequences by PCR. Antibody was measured by two independently designed ELISA serology tests. HEV RNA fragments were amplified by RT‐PCR of nucleic acid extracted from faeces of 10–12‐week‐old piglets using primers targeting ORF1, ORF2, and ORF2/3. PCR products were subject to phylogenetic analysis. Antibody to HEV was found throughout New Zealand pig herds as well as in the different age groups within the herds. Twenty herds from 22 tested were positive for HEV antibody (91% herd prevalence). Phylogenetic analysis of the amplified sequences placed this New Zealand strain of HEV closest to the human European strain It‐1 (AF 110390) and U.S. swine strain (AF 082843) with 88% and 83% similarity respectively in ORF1. It was concluded that HEV is widely distributed in the New Zealand pig population. Phylogenetic analysis shows that this is a new HEV strain, grouping most closely with the United States/European cluster, which includes HEV strains of both human and swine origin. J. Med. Virol. 65:525–529, 2001. © 2001 Wiley‐Liss, Inc.
Bibliography:	istex:4FFB56407A1D2F85C3FAEA8A3F332A8B1B2316E3 ArticleID:JMV2067 Virology and Immunology Laboratory, Auckland Hospital, New Zealand ark:/67375/WNG-8WC4HJHM-5 Hepatitis Branch, Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	0146-6615 1096-9071
DOI:	10.1002/jmv.2067