Hepatoprotective and in vivo antioxidant activities of the hydroethanolic leaf extract of Mucuna pruriens (Fabaceae) in antitubercular drugs and alcohol models

Hepatotoxicity is a significantly increasing health problem worldwide, and the extent of the problem has stimulated interest in the search for hepatotherapeutic agents from plants. This study investigated the hepatoprotective and in vivo antioxidant activities of the hydroethanolic extract of Mucuna...

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Published in:	Chinese journal of natural medicines Vol. 12; no. 4; pp. 273 - 283
Main Authors:	Obogwu, Mercy B., Akindele, Abidemi J., Adeyemi, Olufunmilayo O.
Format:	Journal Article
Language:	English
Published:	China Elsevier B.V 01-04-2014
Subjects:	Alanine Transaminase - metabolism Alcohol Animals Antioxidant Antioxidants - administration & dosage Antitubercular Agents - toxicity Antitubercular drugs Aspartate Aminotransferases - metabolism Catalase - metabolism Chemical and Drug Induced Liver Injury - enzymology Chemical and Drug Induced Liver Injury - prevention & control Ethanol - toxicity Fabaceae Female Glutathione Peroxidase - metabolism Hepatoprotective Humans Liver - drug effects Liver - enzymology Liver Diseases, Alcoholic - enzymology Liver Diseases, Alcoholic - prevention & control Male Malondialdehyde - metabolism Mucuna - chemistry Mucuna pruriens Plant Extracts - administration & dosage Plant Leaves - chemistry Protective Agents - administration & dosage Rats, Wistar Superoxide Dismutase - metabolism Antitubercular drugs Alcohol Hepatoprotective Mucuna pruriens Antioxidant Fabaceae
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Summary:	Hepatotoxicity is a significantly increasing health problem worldwide, and the extent of the problem has stimulated interest in the search for hepatotherapeutic agents from plants. This study investigated the hepatoprotective and in vivo antioxidant activities of the hydroethanolic extract of Mucuna pruriens leaves in antitubercular and alcohol-induced hepatotoxicity assays in rats. In each of the models used, seven groups were allotted. The different groups received normal saline (10 mL·kg−1, p.o.); hepatotoxicant (isoniazid-rifampicin, INH-RIF, 100 mg·kg−1, i.p. or 20% ethanol 5 g·kg−1, p.o.) and normal saline (10 mL·kg−1, p.o.); hepatotoxicant and extract at doses of 100, 200, and 400 mg·kg−1 p.o.; hepatotoxicant and silymarin 50 mg·kg−1 p.o.; and extract at 400 mg·kg−1 p.o. On the 21st day of treatment, blood was collected for assessment of serum biochemical parameters and harvested liver samples were assessed for antioxidants. The hepatotoxicants significantly (P < 0.05−0.001) increased the levels of alanine transaminase (ALT), aspartate transaminase (AST), alkaline phosphatase (ALP), bilirubin, and malondialdehyde (MDA); and reduced the levels of catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx), and reduced glutathione GSH compared to control. M. pruriens significantly reversed (P < 0.05−0.001) the elevation in the level of ALT, AST, ALP, and bilirubin caused by the hepatotoxicants. The extract (200 and 400 mg·kg−1) significantly reversed (P < 0.05) the diminution in the level of in vivo antioxidants and increased the level of MDA produced by INH-RIF. M. pruriens (100−400 mg·kg−1) elicited significant reduction (P < 0.001) in the level of MDA compared to the alcohol group. Silymarin also reversed the deleterious effects of the hepatotoxicants. The hydroethanolic extract of Mucuna pruriens leaves possesses hepatoprotective activity with enhancement of in vivo antioxidants as a possible mechanism of action.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1875-5364 1875-5364
DOI:	10.1016/S1875-5364(14)60054-6