Soluble CD154 in rheumatoid arthritis: elevated plasma levels in cases with vasculitis

Soluble CD154 in rheumatoid arthritis: elevated plasma levels in cases with vasculitis

OBJECTIVE: To determine the levels of soluble CD154 (sCD154) in the plasma of patients with rheumatoid arthritis (RA) and rheumatoid vasculitis (RV). and to examine the relationship between the levels of sCD154 in plasma and the clinical variables. METHODS: Levels of sCD154 were quantified in 39 pla...

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Bibliographic Details
Published in:Journal of rheumatology Vol. 28; no. 12; pp. 2583 - 2590
Main Authors: TAMURA, Naoto, KOBAYASHI, Shigeto, KATO, Kazunori, BANDO, Hideaki, HARUTA, Kazuhiko, OYANAGI, Mutsuko, KURIYAMA, Makiyo, KIPPS, Thomas J, HASHIMOTO, Hiroshi
Format: Journal Article
Language:English
Published: Toronto, ON The Journal of Rheumatology 01-12-2001
Journal of Rheumatology Publishing
Subjects:
Adult
Aged
Arthritis, Rheumatoid - complications
Arthritis, Rheumatoid - immunology
Biological and medical sciences
CD40 Ligand - blood
Diseases of the osteoarticular system
Enzyme-Linked Immunosorbent Assay
Female
Humans
Immunoglobulin G - blood
Immunoglobulin M - blood
Inflammatory joint diseases
Male
Medical sciences
Middle Aged
Rheumatoid Factor - blood
Rheumatoid Factor - classification
Vasculitis - complications
Vasculitis - immunology
Human
Immunopathology
Pathogenesis
Diseases of the osteoarticular system
Cardiovascular disease
Autoimmune disease
Inflammatory joint disease
Systemic
Concentration
Soluble form
Blood plasma
Vascular disease
Rheumatoid factor
Symptomatology
Chronic
Vasculitis
Correlation analysis
Rheumatoid arthritis
Systemic disease
Complication
ELISA assay
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Summary:OBJECTIVE: To determine the levels of soluble CD154 (sCD154) in the plasma of patients with rheumatoid arthritis (RA) and rheumatoid vasculitis (RV). and to examine the relationship between the levels of sCD154 in plasma and the clinical variables. METHODS: Levels of sCD154 were quantified in 39 plasma samples from patients with RA, including 9 patients who were also diagnosed with RV, and compared with those of 20 healthy subjects. An ELISA was established and specificity of the ELISA was tested by control ELISA using isotype-matched IgG and preabsorption assay. The titers of IgM and IgG rheumatoid factor (IgM-RF, IgG-RF) for each patient were determined simultaneously, and values of other laboratory variables were also determined. RESULTS: Levels of sCD 154 in plasma were higher in patients with RA than in the healthy subjects (p < 0.02). Compared with RA patients without vasculitis, patients with RV had significantly higher levels of sCD154 in their plasma (p < 0.001). Control ELISA and absorption assay of sCD154 indicated that our ELISA system was capable of measuring plasma sCD154 in RA patients. Levels of sCD154 in RA plasma correlated significantly with both IgM-RF and IgG-RF titers (r = 0.64 and 0.61, respectively, both p < 0.001). The levels of sCD154 decreased after commencement of treatment for vasculitis in cases with RV. CONCLUSION: We identified the presence of sCD154 in RA plasma, with especially high levels in cases with vasculitis. Correlation between sCD154 and RF titers indicates the CD154-CD40 pathway is likely related to pathogenic RF production.
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ISSN:0315-162X
1499-2752