Search Results - "ORKIN, Stuart H"

Chromatin Connections to Pluripotency and Cellular Reprogramming by Orkin, Stuart H., Hochedlinger, Konrad

“…The pluripotent state of embryonic stem cells (ESCs) provides a unique perspective on regulatory programs that govern self-renewal and differentiation and…”
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Emerging Genetic Therapy for Sickle Cell Disease by Orkin, Stuart H, Bauer, Daniel E

“…The genetic basis of sickle cell disease (SCD) was elucidated >60 years ago, yet current therapy does not rely on this knowledge. Recent advances raise…”
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Opposing Roles for the lncRNA Haunt and Its Genomic Locus in Regulating HOXA Gene Activation during Embryonic Stem Cell Differentiation by Yin, Yafei, Yan, Pixi, Lu, Jinlong, Song, Guang, Zhu, Yangyang, Li, Zhaohui, Zhao, Yi, Shen, Bin, Huang, Xingxu, Zhu, Heng, Orkin, Stuart H., Shen, Xiaohua

“…Long noncoding RNAs (lncRNAs) have been implicated in controlling various aspects of embryonic stem cell (ESC) biology, although the functions of specific…”
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Hemoglobin switching's surprise: the versatile transcription factor BCL11A is a master repressor of fetal hemoglobin by Bauer, Daniel E, Orkin, Stuart H

“…The major disorders of β-globin, sickle cell disease and β-thalassemia, may be ameliorated by expression of the fetal gene paralog γ-globin. Uncertainty…”
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Mapping the Mouse Cell Atlas by Microwell-Seq by Han, Xiaoping, Wang, Renying, Zhou, Yincong, Fei, Lijiang, Sun, Huiyu, Lai, Shujing, Saadatpour, Assieh, Zhou, Ziming, Chen, Haide, Ye, Fang, Huang, Daosheng, Xu, Yang, Huang, Wentao, Jiang, Mengmeng, Jiang, Xinyi, Mao, Jie, Chen, Yao, Lu, Chenyu, Xie, Jin, Fang, Qun, Wang, Yibin, Yue, Rui, Li, Tiefeng, Huang, He, Orkin, Stuart H., Yuan, Guo-Cheng, Chen, Ming, Guo, Guoji

“…Single-cell RNA sequencing (scRNA-seq) technologies are poised to reshape the current cell-type classification system. However, a transcriptome-based…”
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An Engineered CRISPR-Cas9 Mouse Line for Simultaneous Readout of Lineage Histories and Gene Expression Profiles in Single Cells by Bowling, Sarah, Sritharan, Duluxan, Osorio, Fernando G., Nguyen, Maximilian, Cheung, Priscilla, Rodriguez-Fraticelli, Alejo, Patel, Sachin, Yuan, Wei-Chien, Fujiwara, Yuko, Li, Bin E., Orkin, Stuart H., Hormoz, Sahand, Camargo, Fernando D.

“…Tracing the lineage history of cells is key to answering diverse and fundamental questions in biology. Coupling of cell ancestry information with other…”
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Polycomb Repressive Complex 2 Regulates Normal Hematopoietic Stem Cell Function in a Developmental-Stage-Specific Manner by Xie, Huafeng, Xu, Jian, Hsu, Jessie H., Nguyen, Minh, Fujiwara, Yuko, Peng, Cong, Orkin, Stuart H.

“…Recent studies point to a pivotal role of Polycomb repressive complex 2 (PRC2) in stem cell function and cancer. Loss-of-function approaches targeting…”
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Direct Promoter Repression by BCL11A Controls the Fetal to Adult Hemoglobin Switch by Liu, Nan, Hargreaves, Victoria V., Zhu, Qian, Kurland, Jesse V., Hong, Jiyoung, Kim, Woojin, Sher, Falak, Macias-Trevino, Claudio, Rogers, Julia M., Kurita, Ryo, Nakamura, Yukio, Yuan, Guo-Cheng, Bauer, Daniel E., Xu, Jian, Bulyk, Martha L., Orkin, Stuart H.

“…Fetal hemoglobin (HbF, α2γ2) level is genetically controlled and modifies severity of adult hemoglobin (HbA, α2β2) disorders, sickle cell disease, and…”
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Dissecting super-enhancer hierarchy based on chromatin interactions by Huang, Jialiang, Li, Kailong, Cai, Wenqing, Liu, Xin, Zhang, Yuannyu, Orkin, Stuart H., Xu, Jian, Yuan, Guo-Cheng

“…Recent studies have highlighted super-enhancers (SEs) as important regulatory elements for gene expression, but their intrinsic properties remain incompletely…”
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BCL11A enhancer dissection by Cas9-mediated in situ saturating mutagenesis by Canver, Matthew C., Smith, Elenoe C., Sher, Falak, Pinello, Luca, Sanjana, Neville E., Shalem, Ophir, Chen, Diane D., Schupp, Patrick G., Vinjamur, Divya S., Garcia, Sara P., Luc, Sidinh, Kurita, Ryo, Nakamura, Yukio, Fujiwara, Yuko, Maeda, Takahiro, Yuan, Guo-Cheng, Zhang, Feng, Orkin, Stuart H., Bauer, Daniel E.

“…Enhancers, critical determinants of cellular identity, are commonly recognized by correlative chromatin marks and gain-of-function potential, although only…”
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Live-animal imaging of native haematopoietic stem and progenitor cells by Christodoulou, Constantina, Spencer, Joel A., Yeh, Shu-Chi A., Turcotte, Raphaël, Kokkaliaris, Konstantinos D., Panero, Riccardo, Ramos, Azucena, Guo, Guoji, Seyedhassantehrani, Negar, Esipova, Tatiana V., Vinogradov, Sergei A., Rudzinskas, Sarah, Zhang, Yi, Perkins, Archibald S., Orkin, Stuart H., Calogero, Raffaele A., Schroeder, Timm, Lin, Charles P., Camargo, Fernando D.

“…The biology of haematopoietic stem cells (HSCs) has predominantly been studied under transplantation conditions 1 , 2 . It has been particularly challenging to…”
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Reprogramming Committed Murine Blood Cells to Induced Hematopoietic Stem Cells with Defined Factors by Riddell, Jonah, Gazit, Roi, Garrison, Brian S., Guo, Guoji, Saadatpour, Assieh, Mandal, Pankaj K., Ebina, Wataru, Volchkov, Pavel, Yuan, Guo-Cheng, Orkin, Stuart H., Rossi, Derrick J.

“…Hematopoietic stem cells (HSCs) sustain blood formation throughout life and are the functional units of bone marrow transplantation. We show that transient…”
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An Extended Transcriptional Network for Pluripotency of Embryonic Stem Cells by Kim, Jonghwan, Chu, Jianlin, Shen, Xiaohua, Wang, Jianlong, Orkin, Stuart H.

“…Much attention has focused on a small set of transcription factors that maintain human or mouse embryonic stem (ES) cells in a pluripotent state. To gain a…”
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Mapping the Mouse Cell Atlas by Microwell-Seq by Han, Xiaoping, Wang, Renying, Zhou, Yincong, Fei, Lijiang, Sun, Huiyu, Lai, Shujing, Saadatpour, Assieh, Zhou, Ziming, Chen, Haide, Ye, Fang, Huang, Daosheng, Xu, Yang, Huang, Wentao, Jiang, Mengmeng, Jiang, Xinyi, Mao, Jie, Chen, Yao, Lu, Chenyu, Xie, Jin, Fang, Qun, Wang, Yibin, Yue, Rui, Li, Tiefeng, Huang, He, Orkin, Stuart H., Yuan, Guo-Cheng, Chen, Ming, Guo, Guoji

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Targeted disruption of the EZH2–EED complex inhibits EZH2-dependent cancer by Kim, Woojin, Bird, Gregory H, Neff, Tobias, Guo, Guoji, Kerenyi, Marc A, Walensky, Loren D, Orkin, Stuart H

“…EZH2 and EED are components of the Polycomb repressive complex 2 (PRC2), a ‘writer’ complex involved in histone methylation. A stapled peptide that disrupts…”
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Mapping Cellular Hierarchy by Single-Cell Analysis of the Cell Surface Repertoire by Guo, Guoji, Luc, Sidinh, Marco, Eugenio, Lin, Ta-Wei, Peng, Cong, Kerenyi, Marc A., Beyaz, Semir, Kim, Woojin, Xu, Jian, Das, Partha Pratim, Neff, Tobias, Zou, Keyong, Yuan, Guo-Cheng, Orkin, Stuart H.

“…Stem cell differentiation pathways are most often studied at the population level, whereas critical decisions are executed at the level of single cells. We…”
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A Myc Network Accounts for Similarities between Embryonic Stem and Cancer Cell Transcription Programs by Kim, Jonghwan, Woo, Andrew J., Chu, Jianlin, Snow, Jonathan W., Fujiwara, Yuko, Kim, Chul Geun, Cantor, Alan B., Orkin, Stuart H.

“…c-Myc (Myc) is an important transcriptional regulator in embryonic stem (ES) cells, somatic cell reprogramming, and cancer. Here, we identify a Myc-centered…”
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Developmental Control of Polycomb Subunit Composition by GATA Factors Mediates a Switch to Non-Canonical Functions by Xu, Jian, Shao, Zhen, Li, Dan, Xie, Huafeng, Kim, Woojin, Huang, Jialiang, Taylor, Jordan E., Pinello, Luca, Glass, Kimberly, Jaffe, Jacob D., Yuan, Guo-Cheng, Orkin, Stuart H.

“…Polycomb repressive complex 2 (PRC2) plays crucial roles in transcriptional regulation and stem cell development. However, the context-specific functions…”
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Dynamic Control of Enhancer Repertoires Drives Lineage and Stage-Specific Transcription during Hematopoiesis by Huang, Jialiang, Liu, Xin, Li, Dan, Shao, Zhen, Cao, Hui, Zhang, Yuannyu, Trompouki, Eirini, Bowman, Teresa V., Zon, Leonard I., Yuan, Guo-Cheng, Orkin, Stuart H., Xu, Jian

“…Enhancers are the primary determinants of cell identity, but the regulatory components controlling enhancer turnover during lineage commitment remain largely…”
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Tet1 and Tet2 Regulate 5-Hydroxymethylcytosine Production and Cell Lineage Specification in Mouse Embryonic Stem Cells by Koh, Kian Peng, Yabuuchi, Akiko, Rao, Sridhar, Huang, Yun, Cunniff, Kerrianne, Nardone, Julie, Laiho, Asta, Tahiliani, Mamta, Sommer, Cesar A., Mostoslavsky, Gustavo, Lahesmaa, Riitta, Orkin, Stuart H., Rodig, Scott J., Daley, George Q., Rao, Anjana

“…TET family enzymes convert 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC) in DNA. Here, we show that Tet1 and Tet2 are Oct4-regulated enzymes that…”
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