Exploring the possibility of a proprietary black cumin oil extract as a Dual Orexin Receptor Antagonist in restoring stress-sleep balance on stress-induced sleep deprived animals

Exploring the possibility of a proprietary black cumin oil extract as a Dual Orexin Receptor Antagonist in restoring stress-sleep balance on stress-induced sleep deprived animals

Orexins act as a molecular switch for the release of cortisol/corticosterone in response to a stress stimulus and hence to regulate sleep/wake cycle. Orexin agonism during the day promotes wakefulness, and Dual Orexin Receptor Antagonists (DORA) can promote sleep signals by enhancing melatonin, whic...

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Bibliographic Details
Published in:PharmaNutrition Vol. 26; p. 100357
Main Authors: OR, Saneha, Krishna, Priya, Das S, Syam, IM, Krishnakumar, Joseph, Litty
Format: Journal Article
Language:English
Published: Elsevier B.V 01-12-2023
Subjects:
Black cumin oil
BlaQmax
DORA
Nigella sativa
Sleep
Black cumin oil
Nigella sativa
ACTH
HPA axis
MT1
OX2R
PDA
BlaQmax
OX1R
COVID-19
Hz
Sleep
IAEC
ANOVA
BCO-5
CRH
GABA
NMDA
DORA
qPCR
OFT
TQ
GAPDH
HPLC
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Summary:Orexins act as a molecular switch for the release of cortisol/corticosterone in response to a stress stimulus and hence to regulate sleep/wake cycle. Orexin agonism during the day promotes wakefulness, and Dual Orexin Receptor Antagonists (DORA) can promote sleep signals by enhancing melatonin, which is an inhibitor of orexin. It was reported that a proprietary black cumin (Nigella sativa) oil (BCO-5) alleviated stress and improved sleep quality. The present study investigated the mechanism of action of BCO-5 using stress-induced and sleep-deprived model of rats. Adult Sprague Dawley rats (n = 24) were randomised into 4 groups (Group I – Sham; Group II – Stress-induced group; Group III – BCO-5 treated normal animals; Group IV – Stress + BCO-5 (20 mg/kg b. wt.) for 14 days and monitored the behaviour and biochemical markers. The co-supplementation of BCO-5 significantly decreased the body weight, locomotor activity, rearing and grooming frequencies among Group IV animals significantly compared to Group II. The observed behaviour was also correlated with the significant decrease in orexin, corticosterone and c-fos expression levels, while an increase was observed in melatonin concentration. Our results support the plausible role of BCO-5 as a DORA to manage stress and improve sleep. [Display omitted] •BCO-5 may act as a Dual Orexin Receptor antagonist.•BCO-5 modulates HPA axis to balance corticosterone and melatonin.•BCO-5 reduces corticosterone and improves stress-induced sleep issues.•BCO-5 may regulate sleep/wake cycles.
ISSN:2213-4344
2213-4344
DOI:10.1016/j.phanu.2023.100357