Search Results - "OHTSUKI, Sumio"

Changes of Blood-Brain Barrier and Brain Parenchymal Protein Expression Levels of Mice under Different Insulin-Resistance Conditions Induced by High-Fat Diet by Ogata, Seiryo, Ito, Shingo, Masuda, Takeshi, Ohtsuki, Sumio

“…Purpose The purpose of the present study was to investigate changes of blood-brain barrier (BBB) and brain parenchymal protein expression due to type II…”
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Reduction in hepatic secondary bile acids caused by short-term antibiotic-induced dysbiosis decreases mouse serum glucose and triglyceride levels by Kuno, Takuya, Hirayama-Kurogi, Mio, Ito, Shingo, Ohtsuki, Sumio

“…Antibiotic-caused changes in intestinal flora (dysbiosis) can have various effects on the host. Secondary bile acids produced by intestinal bacteria are…”
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Efficient isolation of brain capillary from a single frozen mouse brain for protein expression analysis by Ogata, Seiryo, Ito, Shingo, Masuda, Takeshi, Ohtsuki, Sumio

“…Isolated brain capillaries are essential for analyzing the changes of protein expressions at the blood–brain barrier (BBB) under pathological conditions. The…”
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Effect of Insulin Receptor-Knockdown on the Expression Levels of Blood–Brain Barrier Functional Proteins in Human Brain Microvascular Endothelial Cells by Nagano, Hinako, Ito, Shingo, Masuda, Takeshi, Ohtsuki, Sumio

“…Purpose The insulin receptor (INSR) mediates insulin signaling to modulate cellular functions. Although INSR is expressed at the blood–brain barrier (BBB), its…”
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Quantitative targeted absolute proteomics of human blood–brain barrier transporters and receptors by Uchida, Yasuo, Ohtsuki, Sumio, Katsukura, Yuki, Ikeda, Chiemi, Suzuki, Takashi, Kamiie, Junichi, Terasaki, Tetsuya

“…J. Neurochem. (2011) 117, 333–345. We have obtained, for the first time, a quantitative protein expression profile of membrane transporters and receptors in…”
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Effect of Intestinal Flora on Protein Expression of Drug-Metabolizing Enzymes and Transporters in the Liver and Kidney of Germ-Free and Antibiotics-Treated Mice by Kuno, Takuya, Hirayama-Kurogi, Mio, Ito, Shingo, Ohtsuki, Sumio

“…Dysbiosis (alteration of intestinal flora) is associated with various host physiologies, including diseases. The purpose of this study was to clarify the…”
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Human Hepatic Transporter Signature Peptides for Quantitative Targeted Absolute Proteomics: Selection, Digestion Efficiency, and Peptide Stability by Mori, Ayano, Masuda, Takeshi, Ito, Shingo, Ohtsuki, Sumio

“…Purpose Quantitative targeted absolute proteomics (QTAP) quantifies proteins by measuring the signature peptides produced from target proteins by trypsin…”
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Matrix mechanotransduction mediated by thrombospondin-1/integrin/YAP in the vascular remodeling by Yamashiro, Yoshito, Thang, Bui Quoc, Ramirez, Karina, Shin, Seung Jae, Kohata, Tomohiro, Ohata, Shigeaki, Nguyen, Tram Anh Vu, Ohtsuki, Sumio, Nagayama, Kazuaki, Yanagisawa, Hiromi

“…The extracellular matrix (ECM) initiates mechanical cues that activate intracellular signaling through matrix–cell interactions. In blood vessels, additional…”
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Diurnal Changes in Protein Expression at the Blood–Brain Barrier in Mice by Ogata, Seiryo, Ito, Shingo, Masuda, Takeshi, Ohtsuki, Sumio

“…Circadian rhythms influence the transport function of the blood–brain barrier (BBB) and peripheral organs. However, the influence of circadian rhythms on…”
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Quantitative Determination of Luminal and Abluminal Membrane Distributions of Transporters in Porcine Brain Capillaries by Plasma Membrane Fractionation and Quantitative Targeted Proteomics by Kubo, Yoshiyuki, Ohtsuki, Sumio, Uchida, Yasuo, Terasaki, Tetsuya

“…Abluminal or luminal localization of transporter in plasma membranes at the blood-brain barrier (BBB) is critical for their physiological and pharmacological…”
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Quantitative atlas of blood-brain barrier transporters, receptors, and tight junction proteins in rats and common marmoset by Hoshi, Yutaro, Uchida, Yasuo, Tachikawa, Masanori, Inoue, Takashi, Ohtsuki, Sumio, Terasaki, Tetsuya

“…The purpose of this study was to determine the protein amounts of blood-brain barrier (BBB) permeability-related transporters, receptors, and tight junction…”
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In-vitro acetylation of SARS-CoV and SARS-CoV-2 nucleocapsid proteins by human PCAF and GCN5 by Hatakeyama, Dai, Masuda, Takeshi, Miki, Ryosuke, Ohtsuki, Sumio, Kuzuhara, Takashi

“…Recently, the novel coronavirus (SARS-CoV-2), which has spread from China to the world, was declared a global public health emergency, which causes lethal…”
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Multi-laboratory assessment of reproducibility, qualitative and quantitative performance of SWATH-mass spectrometry by Collins, Ben C., Hunter, Christie L., Liu, Yansheng, Schilling, Birgit, Rosenberger, George, Bader, Samuel L., Chan, Daniel W., Gibson, Bradford W., Gingras, Anne-Claude, Held, Jason M., Hirayama-Kurogi, Mio, Hou, Guixue, Krisp, Christoph, Larsen, Brett, Lin, Liang, Liu, Siqi, Molloy, Mark P., Moritz, Robert L., Ohtsuki, Sumio, Schlapbach, Ralph, Selevsek, Nathalie, Thomas, Stefani N., Tzeng, Shin-Cheng, Zhang, Hui, Aebersold, Ruedi

“…Quantitative proteomics employing mass spectrometry is an indispensable tool in life science research. Targeted proteomics has emerged as a powerful approach…”
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Quantitative and targeted proteomics-based identification and validation of drug efficacy biomarkers by Masuda, Takeshi, Mori, Ayano, Ito, Shingo, Ohtsuki, Sumio

“…Proteomics refers to the large-scale study of proteins, providing comprehensive and quantitative information on proteins in tissue, blood, and cell samples. In…”
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Quantitative targeted absolute proteomics-based ADME research as a new path to drug discovery and development: methodology, advantages, strategy, and prospects by Ohtsuki, Sumio, Uchida, Yasuo, Kubo, Yoshiyuki, Terasaki, Tetsuya

“…An understanding of the functional roles of proteins, for example, in drug absorption, distribution, metabolism, elimination, toxicity, and efficacy…”
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Blood-brain barrier (BBB) pharmacoproteomics: reconstruction of in vivo brain distribution of 11 P-glycoprotein substrates based on the BBB transporter protein concentration, in vitro intrinsic transport activity, and unbound fraction in plasma and brain in mice by Uchida, Yasuo, Ohtsuki, Sumio, Kamiie, Junichi, Terasaki, Tetsuya

“…The purpose of this study was to examine whether in vivo drug distribution to the brain can be reconstructed by integrating P-glycoprotein (P-gp)/mdr1a…”
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Mass Spectrometry-Compatible Subcellular Fractionation for Proteomics by Masuda, Takeshi, Sugiyama, Naoyuki, Tomita, Masaru, Ohtsuki, Sumio, Ishihama, Yasushi

“…We found that nuclear envelopes stabilize against surfactants in the presence of ethylene glycol (EG). We, therefore, developed a novel subcellular…”
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Major involvement of Na+‐dependent multivitamin transporter (SLC5A6/SMVT) in uptake of biotin and pantothenic acid by human brain capillary endothelial cells by Uchida, Yasuo, Ito, Katsuaki, Ohtsuki, Sumio, Kubo, Yoshiyuki, Suzuki, Takashi, Terasaki, Tetsuya

“…The purpose of this study was to clarify the expression of Na+‐dependent multivitamin transporter (SLC5A6/SMVT) and its contribution to the supply of biotin…”
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Identification of blood biomarkers in glioblastoma by SWATH mass spectrometry and quantitative targeted absolute proteomics by Miyauchi, Eisuke, Furuta, Takuya, Ohtsuki, Sumio, Tachikawa, Masanori, Uchida, Yasuo, Sabit, Hemragul, Obuchi, Wataru, Baba, Tomoko, Watanabe, Michitoshi, Terasaki, Tetsuya, Nakada, Mitsutoshi

“…Molecular biomarkers in blood are needed to aid the early diagnosis and clinical assessment of glioblastoma (GBM). Here, in order to identify biomarker…”
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The blood‐brain barrier fatty acid transport protein 1 (FATP1/SLC27A1) supplies docosahexaenoic acid to the brain, and insulin facilitates transport by Ochiai, Yusuke, Uchida, Yasuo, Ohtsuki, Sumio, Tachikawa, Masanori, Aizawa, Sanshiro, Terasaki, Tetsuya

“…We purposed to clarify the contribution of fatty acid transport protein 1 (FATP1/SLC 27A1) to the supply of docosahexaenoic acid (DHA) to the brain across the…”
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