Characterization of West Nile Viruses Isolated from Captive American Flamingoes (Phoenicopterus ruber) in Medellin, Colombia

Serum samples from a total of 71 healthy captive birds belonging to 18 species were collected in July of 2008 in Medellin (Colombia) and tested for flaviviruses. Eighteen of 29 samples from American Flamingoes (Phoenicopterus ruber) were positive for West Nile virus (WNV) by reverse transcription-po...

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Bibliographic Details
Published in:	The American journal of tropical medicine and hygiene Vol. 87; no. 3; pp. 565 - 572
Main Authors:	OSORIO, Jorge E, CIUODERIS, Karl A, LOPERA, Juan G, PIEDRAHITA, Leidy D, MURPHY, Darby, LEVASSEUR, James, CARRILLO, Lina, OCAMPO, Martha C, HOFMEISTER, Erik
Format:	Journal Article
Language:	English
Published:	Deerfield, IL American Society of Tropical Medecine and Hygiene 01-09-2012 The American Society of Tropical Medicine and Hygiene
Subjects:	Animals Arboviral encephalitis Arboviroses Biological and medical sciences Bird Diseases - virology Birds - virology Chickens - virology Colombia Human viral diseases Infectious diseases Louisiana Medical sciences Mice Mice, Inbred BALB C Miscellaneous Phenotype Reverse Transcriptase Polymerase Chain Reaction RNA, Viral - genetics RNA, Viral - isolation & purification Texas Tropical viral diseases Viral diseases West Nile Fever - genetics West Nile Fever - veterinary West Nile Fever - virology West Nile virus - classification West Nile virus - genetics West Nile virus - isolation & purification South America Colombia America Louisiana Texas Infection Nervous system diseases West Nile encephalitis Viral disease Arbovirus disease Central nervous system disease Tropical medicine Cerebral disorder
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Summary:	Serum samples from a total of 71 healthy captive birds belonging to 18 species were collected in July of 2008 in Medellin (Colombia) and tested for flaviviruses. Eighteen of 29 samples from American Flamingoes (Phoenicopterus ruber) were positive for West Nile virus (WNV) by reverse transcription-polymerase chain reaction. Selected positive samples were serially passaged and WNV was confirmed by immunofluorescence. Two isolates (524/08, 9835/08) were characterized in vitro and in vivo. Sequence analysis revealed WNV with 16 nucleotide substitutions resulting in six amino acid changes when compared with the NY99 strain. Colombian (COL) viruses were more closely related to Louisiana isolates from 2001. When compared with attenuated strains isolated from Texas, COL isolates differed in their plaque size and temperature sensitivity phenotype. The COL viruses were pathogenic in embryonated chicken eggs and Balb/c mice.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0002-9637 1476-1645
DOI:	10.4269/ajtmh.2012.11-0655