Effects of acute fasting and age on leptin and peroxisome proliferator‐activated receptor gamma production relative to fat depot in immature and mature pigs

Effects of acute fasting and age on leptin and peroxisome proliferator‐activated receptor gamma production relative to fat depot in immature and mature pigs

Leptin and peroxisome proliferator‐activated receptor gamma (PPARγ) are adipogenic proteins that are actively involved in metabolic homeostasis of fat. Recently, it was reported that fat tissue in humans and rodents differs in metabolic activity relative to anatomical location of the fat tissue (i.e...

Full description

Saved in:
Bibliographic Details
Published in:Journal of animal physiology and animal nutrition Vol. 94; no. 6; pp. e266 - e276
Main Authors: O'Gorman, C.W, Stanko, R.L, Keisler, D.H, Garcia, M.R
Format: Journal Article
Language:English
Published: Oxford, UK Blackwell Publishing Ltd 01-12-2010
Subjects:
Adipogenesis - physiology
Adipose Tissue - metabolism
Aging
Animals
Energy Intake
fasting
fat
Female
Food Deprivation - physiology
leptin
Leptin - blood
Male
pigs
PPAR gamma - blood
PPARγ
Sexual Maturation
Swine - metabolism
Weight Gain
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Leptin and peroxisome proliferator‐activated receptor gamma (PPARγ) are adipogenic proteins that are actively involved in metabolic homeostasis of fat. Recently, it was reported that fat tissue in humans and rodents differs in metabolic activity relative to anatomical location of the fat tissue (i.e. depots) and animal age. Hence, we hypothesized that leptin and PPARγ production in various fat depots in female pigs differs in response to acute fasting, and that these responses vary with physiological maturity of the animal. Sixteen intact crossbred immature female pigs [prepubertal (PP); 132.2 ± 4.1 days] and 16 sexually mature female pigs (M; 224 ± 7.4 days) housed in an open‐air, concrete slab, sheltered barn were randomly assigned to either Control or Fasted treatments. Control pigs (PP, n = 8; M, n = 8) had ad libitum access to feed, while Fasted pigs (PP, n = 8; M, n = 8) were denied access to feed from the onset of the study (0 h) to euthanasia at 72 h. Immediately post‐mortem, fat samples were collected from the subcutaneous, pelvic, kidney, and heart (M pigs only) fat depots and analysed for leptin and PPARγ mRNA and protein content. Acute fasting decreased mean leptin mRNA tissue content in a depot specific manner in M pigs (p < 0.01), while mean leptin protein concentrations in fat tissues did not differ with fat depot or age of the pig. Furthermore, acute fasting did not affect mean PPARγ mRNA tissue content in a fat depot or age dependent manner. Mean concentrations of PPARγ protein in fat depots tended to be greater in M vs. PP pigs (p = 0.07). We suggest that these data provide evidence that acute fasting has a greater effect on leptin than PPARγ production in a fat depot dependent manner in M pigs, which may be indicative of changing physiological demands as an animal matures.
Bibliography:http://dx.doi.org/10.1111/j.1439-0396.2009.00968.x
ark:/67375/WNG-0WGM4TJV-J
istex:027C46259154DAD39FB3E33076C4213431C87A7F
ArticleID:JPN968
ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-News-1
ObjectType-Feature-3
content type line 23
ISSN:0931-2439
1439-0396
DOI:10.1111/j.1439-0396.2009.00968.x