Search Results - "O'Brien, Martha A"

Cell-based bioluminescent assays for all three proteasome activities in a homogeneous format by Moravec, Richard A., O’Brien, Martha A., Daily, William J., Scurria, Michael A., Bernad, Laurent, Riss, Terry L.

“…A luminescent method to individually measure the chymotrypsin-like, trypsin-like, or caspase-like activities of the proteasome in cultured cells was developed…”
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A bioluminescent assay for the sensitive detection of proteases by Leippe, Donna M, Nguyen, Duy, Zhou, Min, Good, Troy, Kirkland, Thomas A, Scurria, Mike, Bernad, Laurent, Ugo, Tim, Vidugiriene, Jolanta, Cali, James J, Klaubert, Dieter H, O'Brien, Martha A

“…A bioluminescent general protease assay was developed using a combination of five luminogenic peptide substrates. The peptide-conjugated luciferin substrates…”
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Homogeneous, bioluminescent proteasome assays by O'Brien, Martha A, Moravec, Richard A, Riss, Terry L, Bulleit, Robert F

“…Protein degradation is mediated predominantly through the ubiquitin-proteasome pathway. The importance of the proteasome in regulating degradation of proteins…”
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Poly (ADP-ribose) polymerase cleavage monitored in situ in apoptotic cells by O'Brien, M A, Moravec, R A, Riss, T L

“…During apoptosis, the activation of a family of cysteine proteases, or caspases, results in proteolytic cleavage of numerous substrates. Antibody probes…”
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Bioluminescent assays for ADMET by Cali, James J, Niles, Andrew, Valley, Michael P, O'Brien, Martha A, Riss, Terry L, Shultz, John

“…Bioluminescent assays couple a limiting component of a luciferase-catalyzed photon-emitting reaction to a variable parameter of interest, while holding the…”
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Multiple Amidated Neuropeptides Are Required for Normal Circadian Locomotor Rhythms in Drosophila by Taghert, Paul H, Hewes, Randall S, Park, Jae H, O'Brien, Martha A, Han, Mei, Peck, Molly E

“…In Drosophila, the amidated neuropeptide pigment dispersing factor (PDF) is expressed by the ventral subset of lateral pacemaker neurons and is required for…”
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Homogeneous, bioluminescent protease assays: caspase-3 as a model by O'Brien, Martha A, Daily, William J, Hesselberth, P Eric, Moravec, Richard A, Scurria, Michael A, Klaubert, Dieter H, Bulleit, Robert F, Wood, Keith V

“…Using caspase-3 as a model, the authors have developed a strategy for highly sensitive, homogeneous protease assays suitable for high-throughput, automated…”
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A peritracheal neuropeptide system in insects: release of myomodulin-like peptides at ecdysis by O'Brien, M A, Taghert, P H

“…We identified of a set of neuropeptide-expressing cells sited along the respiratory system of Drosophila melanogaster using an antibody to the molluscan…”
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Abstract 5595: Rapid and sensitive determination of cytokine release from cells without the need for sample transfer by Lazar, Dan F., Kupcho, Kevin R., Sondgeroth, Casey A., Thompson, David V., O'Brien, Martha A., Gilden, Julia K., Hsiao, Kevin, Cali, James J.

“…Cytokines play a major role in cancer biology as inflammatory and immunomodulatory agents and are frequently measured in cell culture models and other…”
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In situ hybridization analysis of the FMRFamide neuropeptide gene in Drosophila. II. Constancy in the cellular pattern of expression during metamorphosis by O'Brien, M A, Schneider, L E, Taghert, P H

“…We have studied changes in the pattern of specific neuropeptide gene expression during the metamorphosis of the Drosophila nervous system. Prior to…”
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In situ hybridization analysis of the FMRFamide neuropeptide gene in Drosophila. I. Restricted expression in embryonic and larval stages by Schneider, L E, O'Brien, M A, Taghert, P H

“…We have used in situ hybridization techniques to describe the cellular distribution of transcripts from a Drosophila gene that encodes multiple…”
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Lumit: a novel bioluminescent, homogenous immunoassay platform for analyte detection by Bach, Maggie L, O’Brien, Martha A., Nath, Nidhi, Zegzouti, Hicham, Lazar, Dan, Cali, James

“…The detection of a specific analyte in a complex mixture by an immunoassay is a common procedure in many bioanalytical workflows. Immunoassays rely on the…”
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Detecting IL-1β release with a rapid, homogeneous, bioluminescent immunoassay by O’Brien, Martha A., Lindstedt, Anders, Lazar, Dan, Cali, James

“…IL-1β is an important inflammatory cytokine of the innate immune system, released from monocytes, macrophages, and other cell types in response to a variety of…”
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Drosophila MEF2, a transcription factor that is essential for myogenesis by Bour, B A, O'Brien, M A, Lockwood, W L, Goldstein, E S, Bodmer, R, Taghert, P H, Abmayr, S M, Nguyen, H T

“…mef2 encodes the only apparent Drosophila homolog of the vertebrate myocyte-specific enhancer factor 2 (MEF2). We show herein that the Drosophila MEF2 protein…”
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Complementary target engagement and functional assays to probe NLRP3 inflammasome pathway antagonism by O’Brien, Martha A., Robers, Matt, Teske, Kelly, Corona, Cesear, Vasta, Jim, Wilkinson, Jennifer, Lam, Ngan, Hoffman, Kaitlin Dunn, Cali, James, Lazar, Dan

“…Inflammasomes are critical to the innate immune system and implicated in numerous chronic and acute inflammatory diseases. Having tools to screen inflammasome…”
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Homogeneous, bioluminescent proteasome assays by O'Brien, Martha A, Moravec, Richard A, Riss, Terry L, Bulleit, Robert F

“…Protein degradation is mediated predominantly through the ubiquitin-proteasome pathway. The importance of the proteasome in regulating degradation of proteins…”
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Rapid and sensitive determination of cytokine release from cells without the need for sample transfer by Lazar, Dan F, Kupcho, Kevin R, Sondgeroth, Casey A, Hsiao, Kevin, Thompson, David V, O’Brien, Martha A, Gilden, Julia K, Cali, James J

“…We have utilized NanoLuc® Binary Technology (NanoBiT®) to develop a homogeneous and rapid assay method (≤ 70 min completion time) to measure cytokines released…”
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A direct, bioluminescent caspase-1 assay for monitoring inflammasome activation by O’Brien, Martha A., Moehring, Danielle, Munoz-Planillo, Raul, Nunez, Gabriel, Callaway, Justin, Ting, Jenny P, Scurria, Mike, Ugo, Tim, Bernad, Laurent, Cali, James, Lazar, Dan

“…Caspase-1, an essential component of the inflammasome, is activated in response to inflammatory stimuli, resulting in 1) the processing and release of IL-1b…”
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Molecular genetic analysis of the FMRFamide-related neuropeptides in Drosophila by Taghert, P H, O'Brien, M A, Schneider, L E, Roberts, M S

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A kinetic analysis of the action of the insect prothoracicotropic hormone by Bollenbacher, W E, O'Brien, M A, Katahira, E J, Gilbert, L I

“…A modified in vitro assay was used to assess the kinetics of activation and the decay of activation of the prothoracic glands (PG) by the prothoracicotropic…”
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