Prevalence of SGHV among tsetse species of economic importance in Tanzania and their implication for SIT application

[Display omitted] ► Prevalence of GpSGHV is magnified in the lab due to the use of membrane feeding. ► In Tanzania a four-year survey showed virus prevalence in 4species of Glossina. ► Efforts should be expended to use non-infected flies when initiating lab colonies. Sterile Insect technique is an i...

Full description

Saved in:

Bibliographic Details
Published in:	Journal of invertebrate pathology Vol. 112; pp. S133 - S137
Main Authors:	Malele, Imna I., Manangwa, Oliver, Nyingilili, Hamisi H., Kitwika, Winston A., Lyaruu, Eugene A., Msangi, Atway R., Ouma, Johnson O., Nkwangulila, Gamba, Abd-Alla, Adly M.M.
Format:	Journal Article Conference Proceeding
Language:	English
Published:	Amsterdam Elsevier Inc 01-03-2013 Elsevier
Subjects:	Animals Biological and medical sciences coasts DNA Viruses Fundamental and applied biological sciences. Psychology Glossina Glossina fuscipes fuscipes Glossina morsitans Glossina pallidipes Human protozoal diseases hypertrophy In vitro feeding Infectious diseases Insect Viruses Mass rearing Medical sciences Medically important nuisances and vectors, pests of stored products and materials: population survey and control Microbiology Parasitic diseases Pest Control, Biological - economics Pest Control, Biological - methods planning polymerase chain reaction Prevalence Protozoal diseases Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains Salivary gland hyperplasia virus salivary glands seeds Sterile insect technique Tanzania Trypanosomiasis Tsetse Tsetse Flies - virology Vectors. Intermediate hosts Virology viruses Tanzania East Africa Sub-Saharan Africa Africa ISW, Tanzania Mass rearing In vitro feeding Sterile insect technique Tanzania Glossina Tsetse Salivary gland hyperplasia virus Protozoal disease Prevalence Insecta Tropical zone Hyperplasia Sterile animal release Glossinidae Trypanosomiasis Vector Parasite Economic importance Salivary gland Parasitosis African sickness In vitro Feeding Infection Virus Arthropoda Ectoparasite Invertebrata Diptera
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	[Display omitted] ► Prevalence of GpSGHV is magnified in the lab due to the use of membrane feeding. ► In Tanzania a four-year survey showed virus prevalence in 4species of Glossina. ► Efforts should be expended to use non-infected flies when initiating lab colonies. Sterile Insect technique is an important component in area-wide integrated tsetse control. The presence of the salivary glands hypertrophy virus (SGHV) in the wild tsetse, which are the seeds for colony adaptations in the laboratory has become a stumbling block in establishing and maintaining colonies in the laboratory. The virus is transmitted both vertically (in the wild) and horizontally (in the laboratory). However, its prevalence is magnified in the laboratory as a result of the use of in vitro membrane feeding regimen. Fly species of Glossina fuscipes fuscipes, G. pallidipes, G. morsitans and G. swynnertoni were collected from the coastal and inland areas of Tanzania and virus infection rates were assessed microscopically and by PCR. The data showed that in a period of 4years, the virus was present in all species tested irrespective of their ages, sex, and season of the year. However, infection levels differed among species and from one location to another. Symptomatic infection determined by dissection was 1.2% (25/2164) from the coast as compared to 0.4% (6/1725) for inland collected flies. PCR analysis indicated a higher infection rate of 19.81% (104/525) of asymptomatic flies. From these observations, we conclude that care should be taken when planning to initiate tsetse laboratory colonies for use in SIT eradication program. All efforts should be made to select non-infected flies when initiating laboratory colonies and to try to minimize the infection with SGHV. Also management of SGHV infection in the established colony should be applied.
Bibliography:	http://dx.doi.org/10.1016/j.jip.2012.07.018 ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-3 content type line 23 ObjectType-Review-1 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	0022-2011 1096-0805
DOI:	10.1016/j.jip.2012.07.018