Search Results - "Nyberg, Lena"
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Direct identification of antibiotic resistance genes on single plasmid molecules using CRISPR/Cas9 in combination with optical DNA mapping
Published in Scientific reports (01-12-2016)“…Bacterial plasmids are extensively involved in the rapid global spread of antibiotic resistance. We here present an assay, based on optical DNA mapping of…”
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Exploring DNA-protein interactions on the single DNA molecule level using nanofluidic tools
Published in Integrative biology (Cambridge) (14-08-2017)“…DNA-protein interactions are at the core of the cellular machinery and single molecule methods have revolutionized the possibilities to study, and our…”
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Bacteriophage strain typing by rapid single molecule analysis
Published in Nucleic acids research (15-10-2015)“…Rapid characterization of unknown biological samples is under the focus of many current studies. Here we report a method for screening of biological samples by…”
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Nanoconfined Circular and Linear DNA: Equilibrium Conformations and Unfolding Kinetics
Published in Macromolecules (10-02-2015)“…Studies of circular DNA confined to nanofluidic channels are relevant both from a fundamental polymer-physics perspective and due to the importance of circular…”
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Plaque-associated lipids in Alzheimer’s diseased brain tissue visualized by nonlinear microscopy
Published in Scientific reports (27-08-2015)“…By simultaneous coherent anti-Stokes Raman scattering (CARS) and 2-photon fluorescence microscopy of Thioflavin-S stained Alzheimer´s diseased human brain…”
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A single-step competitive binding assay for mapping of single DNA molecules
Published in Biochemical and biophysical research communications (06-01-2012)“…[Display omitted] ► Demonstration of single-step DNA mapping assay with kilobasepair resolution. ► Grayscale fluorescence profile through use of a bright dye…”
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Rapid identification of intact bacterial resistance plasmids via optical mapping of single DNA molecules
Published in Scientific reports (27-07-2016)“…The rapid spread of antibiotic resistance – currently one of the greatest threats to human health according to WHO – is to a large extent enabled by…”
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Facilitated sequence assembly using densely labeled optical DNA barcodes: A combinatorial auction approach
Published in PloS one (09-03-2018)“…The output from whole genome sequencing is a set of contigs, i.e. short non-overlapping DNA sequences (sizes 1-100 kilobasepairs). Piecing the contigs together…”
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Competitive binding-based optical DNA mapping for fast identification of bacteria--multi-ligand transfer matrix theory and experimental applications on Escherichia coli
Published in Nucleic acids research (01-01-2014)“…We demonstrate a single DNA molecule optical mapping assay able to resolve a specific Escherichia coli strain from other strains. The assay is based on…”
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Zebrafish: gaining popularity in lipid research
Published in Biochemical journal (15-07-2010)“…Zebrafish are an increasingly popular vertebrate model organism in which to study biological phenomena. It has been widely used, especially in developmental…”
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Identification and DNA annotation of a plasmid isolated from Chromobacterium violaceum
Published in Scientific reports (28-03-2018)“…Chromobacterium violaceum is a ß-proteobacterium found widely worldwide with important biotechnological properties and is associated to lethal sepsis in…”
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Rapid Tracing of Resistance Plasmids in a Nosocomial Outbreak Using Optical DNA Mapping
Published in ACS infectious diseases (13-05-2016)“…Resistance to life-saving antibiotics increases rapidly worldwide, and multiresistant bacteria have become a global threat to human health. Presently, the most…”
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Applications of optical DNA mapping in microbiology
Published in BioTechniques (01-06-2017)“…Optical mapping (OM) has been used in microbiology for the past 20 years, initially as a technique to facilitate DNA sequence-based studies; however, with…”
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Corrigendum: Rapid identification of intact bacterial resistance plasmids via optical mapping of single DNA molecules
Published in Scientific reports (22-12-2017)“…This corrects the article DOI: 10.1038/srep30410…”
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Assessing How Cationic Intercalators Affect DNA using Nanofluidic Channels
Published in Biophysical Journal (2013)Get full text
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Effect of storage time and temperature on stability of bioactive compounds in aseptically packed beverages prepared from rose hips and sea buckthorn berries
Published in Agricultural and food science (01-01-2015)“…Rose hips and sea buckthorn berries contain high amounts of beneficial bioactive compounds. In this study the content and stability of natural vitamins and…”
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A mutual inhibitory effect on absorption of sphingomyelin and cholesterol
Published in The Journal of nutritional biochemistry (01-05-2000)“…Several studies have shown that there is a strong physical interaction between cholesterol and sphingomyelin (SM). The critical factor is thought to be the…”
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Localization and capacity of sphingomyelin digestion in the rat intestinal tract
Published in The Journal of nutritional biochemistry (01-03-1997)“…Dietary sphingomyelin (SM) undergoes sequential cleavage to ceramide and sphingosine in the intestine. A distinctive intestinal sphingomyelinase (SMase) with…”
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Heterogeneous staining: a tool for studies of how fluorescent dyes affect the physical properties of DNA
Published in Nucleic acids research (01-10-2013)“…The commonly used fluorescent dye YOYO-1 (YOYO) has, using bulk techniques, been demonstrated to stain DNA heterogeneously at substoichiometric concentrations…”
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Sphingomyelin from milk: characterization of liquid crystalline, liposome and emulsion properties
Published in Journal of the American Oil Chemists' Society (01-09-1994)“…The properties of sphingomyelin obtained from bovine milk were investigated. In particular, the properties of liposomes and emulsions prepared from the…”
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