Methodology for processing mastectomy and cryopreservation of breast cancer tissue in a resource- poor setting: A pilot study

There is scarcity of breast cancer tissues derived from women of African origin available for patient - derived xenograft and organoid models. We aim to create a versatile protocol for processing mastectomy and cryopreservation of breast cancer tissue. An immediate collection of breast cancer tissue...

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Bibliographic Details
Published in:	Cryobiology Vol. 97; pp. 179 - 184
Main Authors:	Okoli, Uzoamaka A., Okafor, michael T., Agu, Kenneth A., Ndubuisi, Augustine C., Nwigwe, Ifeoma J., Nna, Emmanuel O., Okafor, Okechukwu C., Ukekwe, Francis I., Nwagha, Teresa U., Menkiti, Victor C., Eze, Charles O., Onyekwelu, Kenechukwu C., Ikekpeazu, Joy E., Anusiem, Chikere A., Mbah, Anthony U., Chijioke, Chioli P., Udeniya, Iroka J.
Format:	Journal Article
Language:	English
Published:	Netherlands Elsevier Inc 01-12-2020
Subjects:	Breast Neoplasms - surgery Cryopreservation - methods Cryoprotective Agents Female Freezing Humans Mastectomy Pilot Projects
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Summary:	There is scarcity of breast cancer tissues derived from women of African origin available for patient - derived xenograft and organoid models. We aim to create a versatile protocol for processing mastectomy and cryopreservation of breast cancer tissue. An immediate collection of breast cancer tissue from mastectomy was bathed in 4 °C HBSS and immediately transferred to 4 °C RPMI1640 containing HEPES, 10% FBS, Streptomycin and Penicillin. Tissues were processed over ice yielding nine samples of cold ischemic time (20–45 min) stored at 3 min interval. Cut samples were transferred into cryovials containing 4 °C cryoprotectant agent (90% FBS +10% Me2SO) before snap -freezing in liquid Nitrogen vapour and final short-term storage in −80 °C Freezer. The histomorphology, tissue and molecular viability were assessed. The cold ischemic times had no detrimental effect to the nine samples despite being processed in a resource poor setting, hence providing a reproducible and reliable protocol.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0011-2240 1090-2392
DOI:	10.1016/j.cryobiol.2020.05.006