Waktu Inkubasi pada Derajat Distilasi Kitosan Enzim dan Efektifitas Penghambatannya terhadap Penyakit Antraknosa

The use of chitosan as a coating agent of harvested fruits is an alternative method in controlling anthracnose disease (Colletotrichum sp.). This study aimed to obtain an optimal enzymatic chitosan (EC) that hydrolyzed using chitinase from Burkholderia cepacia isolate E76. The optimal incubation con...

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Bibliographic Details
Published in:	Jurnal Fitopatologi Vol. 12; no. 6; p. 209
Main Authors:	Suryadi, Yadi, Priyatno, Tri Puji, Samudra, I Made, Susilowati, Dwi Ningsih, Nurzulaika, Hermawati, Syaefudin, Syaefudin
Format:	Journal Article
Language:	English Indonesian
Published:	Perhimpunan Fitopatologi Indonesia 08-03-2017
Subjects:	chilli chitinase chitosan Colletotrichum sp papaya
Online Access:	Get full text
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Summary:	The use of chitosan as a coating agent of harvested fruits is an alternative method in controlling anthracnose disease (Colletotrichum sp.). This study aimed to obtain an optimal enzymatic chitosan (EC) that hydrolyzed using chitinase from Burkholderia cepacia isolate E76. The optimal incubation condition to produce EC was 2 h with the yield of 3.52 ± 0.38 g. The degree of deacetylation (DD) chitosan and EC was 66.91% and 80.91%, respectively. Based on in vitro assays, EC 2% was the most effective in inhibiting the growth of Colletotrichum sp. (94.22%) than chitosan, while the highest inhibition for chitosan 3% was 55.26%. Moreover, the EC 2% showed the highest inhibition of spore germination (74.12%). The in vivo assay revealed that EC 2% showed the highest inhibition on the fungal growth (88.88%), compared to the other concentrations. On the other hand, the EC 2% and 3% gave similar results on inhibition of Colletotrichum sp.of chili (55.55%).
ISSN:	0215-7950 2339-2479
DOI:	10.14692/jfi.12.6.209