Antibody Response of Autogenous Splenic Tissue Implanted in the Abdominal Cavity of Mice

Antibody Response of Autogenous Splenic Tissue Implanted in the Abdominal Cavity of Mice

There is still controversy about the immunologic function of autotransplanted splenic tissue. In this study, splenic autotransplantation was performed in the abdominal cavity of mice, and the plaque‐forming cell (PFC) assay was used to investigate the frequency of antibody‐forming cells in response...

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Bibliographic Details
Published in:World journal of surgery Vol. 29; no. 12; pp. 1623 - 1629
Main Authors: Nunes, Sérgio I., Rezende, Alice B., Teixeira, Francisco M., Ferreira, Ana Paula, Alves, Márcio M.J., Jamel, Nelson, Assis, Raimunda V.C., Teixeira, Henrique C.
Format: Journal Article
Language:English
Published: New York Springer‐Verlag 01-12-2005
Springer
Springer Nature B.V
Subjects:
Animals
Antibody Formation - physiology
Antibody-Producing Cells - physiology
Biological and medical sciences
Cell Count
Female
General aspects
Great Omentum
Hemolytic Plaque Technique
Medical sciences
Mice
Mice, Inbred BALB C
Normal Spleen
Peritoneal Cavity - pathology
Peritoneal Cavity - surgery
Spleen - immunology
Spleen - pathology
Spleen - transplantation
Splenic Autotransplantation
Splenic Tissue
Splenosis - immunology
Splenosis - pathology
Transplantation, Autologous
White Pulp
Spleen
Immune response
Implant
Antibody
Rodentia
Abdomen
Medicine
Tissue
Vertebrata
Mammalia
Treatment
Cavity
Mouse
Implanted
Animal
Surgery
Humoral immunity
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Summary:There is still controversy about the immunologic function of autotransplanted splenic tissue. In this study, splenic autotransplantation was performed in the abdominal cavity of mice, and the plaque‐forming cell (PFC) assay was used to investigate the frequency of antibody‐forming cells in response to sheep red blood cell (SRBC) immunization. BALB/c mice were divided into four groups according to the location of the autogenous graft: intraomental (IO), free peritoneal splenosis (FPS), retroperitoneal (RP), and nongrafted control (CT). Thirty days after surgery the mice were immunized intraperitoneally with SRBCs, and 4 days later splenic immunoglobulin M anti‐SRBC‐secreting cells were determined by counting the number of PFCs. All the immunized mice showed increased numbers of PFCs that were about 2 logs higher than those in the the nonimmunized controls (P < 0.005). The frequencies of anti‐SRBC‐producing cells in the tissues grafted in various sites of the abdominal cavity (IO, FPS, RP), in the normal spleen from nonoperated controls (CT), or in the sham‐operated control group (SCT) were not notably different (5582 ± 2475 PFC/107 cells for IO; 4849 ± 1856 for FPS; 6604 ± 2903 for RP; 5940 ± 5029 for CT; and 6172 ± 2203 for SCT). Similar histology with small architectural variations was observed in all implants; less white pulp was involved, and there was more congestion in the red pulp, with extensive sinusoids and reticular fiber proliferation. This study shows that the T cell‐dependent antibody response in implanted splenic tissues is as efficient as in the intact spleen, with no difference between the graft sites studied. This immune response does not depend on the slight architectural variations observed in the splenic implants.
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ISSN:0364-2313
1432-2323
DOI:10.1007/s00268-005-0060-7